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1. 发明申请

WO2011047307A1 MULTIPLE DISPLACEMENT AMPLIFICATION 审中-公开
标题翻译：多位置放大
公开(公告)号：WO2011047307A1
公开(公告)日：2011-04-21
申请号：PCT/US2010/052904
申请日：2010-10-15
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John , PHILLIPSON, Curtis
发明人： ESHOO, Mark W. , PICURI, John , PHILLIPSON, Curtis
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12Q1/6844 , C12Q1/686 , C12Q2527/125 , C12Q2531/119 , C12Q2537/143 , C12Q2563/159 , C12Q2527/127 , C12Q2531/113
摘要： The present invention provides methods kits and systems for performing multiple displacement amplification reactions. In one method a sample of nucleic acid is provided. The nucleic acid is contacted with a reaction mixture which includes a set of oligonucleotide primers, a one or more polymerase enzymes and a detergent. The reaction mixture is then subjected to conditions under which the nucleic acid sequence is amplified to produce an amplified product in a multiple displacement reaction. The method may also be carried out by contacting the nucleic acid with the reaction mixture in the form of an emulsion. A kit is also provided for carrying out either the methods described above. The kit includes one or more polymerases, a plurality of primers and a detergent. The kit may also include a hydrophobic polymer and may include instructions for performing a multiple displacement amplification reaction on a nucleic acid sample.
摘要翻译：本发明提供了用于进行多重置换扩增反应的方法试剂盒和系统。在一种方法中，提供了核酸样品。将核酸与包含一组寡核苷酸引物，一种或多种聚合酶和洗涤剂的反应混合物接触。然后将反应混合物进行条件，在该条件下扩增核酸序列以在多重置换反应中产生扩增产物。该方法还可以通过使乳酸与乳液形式的反应混合物接触来进行。还提供了用于执行上述方法的试剂盒。试剂盒包括一种或多种聚合酶，多种引物和洗涤剂。试剂盒还可以包括疏水性聚合物，并且可以包括在核酸样品上进行多重置换扩增反应的说明书。

2. 发明申请

WO2013102091A1 NUCLEIC ACID LIGATION SYSTEMS AND METHODS 审中-公开
标题翻译：核酸配位体系及方法
公开(公告)号：WO2013102091A1
公开(公告)日：2013-07-04
申请号：PCT/US2012/072089
申请日：2012-12-28
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PICURI, John , MOTLEY, Stanley , PHILLIPSON, Curtis
发明人： ESHOO, Mark W. , PICURI, John , MOTLEY, Stanley , PHILLIPSON, Curtis
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6855 , C12N15/10 , C12N15/11 , C12N15/66 , C12P19/34 , C12Q1/6869 , C12Q2525/191 , C12Q2525/301 , C12Q2537/143
摘要： The present disclosure relates to systems and methods for nucleic acid ligation. In particular, the present disclosure provides oligonucleotide adaptors for use in nucleic acid ligation reactions.
摘要翻译：本公开涉及用于核酸连接的系统和方法。特别地，本公开提供用于核酸连接反应的寡核苷酸衔接子。

3. 发明申请

WO2012092540A1 METHODS FOR THE SYNTHESIS OF POLYADENYLIC ACID 审中-公开
标题翻译：聚亚氨酸合成方法
公开(公告)号：WO2012092540A1
公开(公告)日：2012-07-05
申请号：PCT/US2011/068049
申请日：2011-12-30
申请人： IBIS BIOSCIENCES, INC. , ESHOO, Mark W. , PHILLIPSON, Curtis
发明人： ESHOO, Mark W. , PHILLIPSON, Curtis
IPC分类号： C12P19/34
CPC分类号： C12P19/34 , C12N9/1258 , C12Y207/07008
摘要： The present invention provides compositions, kits, and methods for synthesizing polyadenylic acid using polynucleotide phosphorylase, adenosine diphosphate, a buffering agent, and a divalent metal cation. In certain embodiments, the adenosine diphosphate is present at a concentration between about 5.0 mM and about 100 mM, and the buffering agent has a pH around 8.0. In some embodiments, the prepared polyadenylic acid compositions are free of detectable nucleic acid.
摘要翻译：本发明提供使用多磷酸化酶，二磷酸腺苷，缓冲剂和二价金属阳离子合成聚腺苷酸的组合物，试剂盒和方法。在某些实施方案中，二磷酸腺苷以约5.0mM至约100mM的浓度存在，并且缓冲剂的pH约为8.0。在一些实施方案中，制备的聚腺苷酸组合物不含可检测的核酸。

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