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1. 发明申请

WO2010118093A2 SCREENING FOR ENVIRONMENTAL DNAS ENCODING ENZYMES FOR SYNTHESIZING TERPENOID-BASED THERAPEUTIC COMPOUNDS USING GENETICALLY MODIFIED E. COLI STRAINS 审中-公开
标题翻译：筛选用于合成基于TERPENOID的治疗化合物的环境DNA编码酶使用遗传修饰的大肠杆菌菌株
公开(公告)号：WO2010118093A2
公开(公告)日：2010-10-14
申请号：PCT/US2010/030181
申请日：2010-04-07
申请人： TUFTS UNIVERSITY , PFEIFER, Blaine , WANG, Yong
发明人： PFEIFER, Blaine , WANG, Yong
IPC分类号： C12N15/70 , C12N15/52 , C12N1/21 , C12Q1/68 , C12Q1/25 , C12R1/19
CPC分类号： C12Q1/6888
摘要： A screening method for identifying microbial genes involved in biosynthesis of therapeutic terpenoid-based compounds using genetically modified E . coli strains that yield high levels of products of the non-mevalonate 1-deoxy-D-xylulose-5-phosphate pathway.
摘要翻译：使用产生高水平的非甲羟戊酸1-脱氧-D-木酮糖-5-磷酸途径的产物的遗传修饰的大肠杆菌菌株鉴定涉及治疗性萜类化合物的生物合成的微生物基因的筛选方法。

2. 发明申请

WO2012166408A2 GENETICALLY MODIFIED E. COLI STRAINS FOR PRODUCING ERYTHROMYCIN ANALOGS 审中-公开
标题翻译：用于生产红霉素类似物的遗传修饰的大肠杆菌菌株
公开(公告)号：WO2012166408A2
公开(公告)日：2012-12-06
申请号：PCT/US2012/038780
申请日：2012-05-21
申请人： TUFTS UNIVERSITY , PFEIFER, Blaine , ZHANG, Haoran , PARK, Sung-Hee , JIANG, Ming
发明人： PFEIFER, Blaine , ZHANG, Haoran , PARK, Sung-Hee , JIANG, Ming
IPC分类号： C12N1/21 , C12N15/31 , C12N15/52 , C12P19/62
CPC分类号： C12N9/1029 , C12N15/52 , C12P19/62 , C12Y203/01094
摘要： Genetically engineered E. coli strains for producing analogs of erythromycin or 6-deoxyerythronolide B. Each of these E. coli strains expresses (a) a modified S. erythraea 6-deoxyerythronolide B (6dEB) synthase capable of synthesizing a 6dEB analog, and/or (b) genes involved in the erythromycin sugar synthesis/attachment/tailoring pathway and those involved in synthesis of one or more non-erythromycin sugar moieties, such as L-olivose, L-oleandrose, D-mycaminose, and pyrrolosamine.
摘要翻译：用于产生红霉素或6-脱氧红霉素B的类似物的遗传工程大肠杆菌菌株这些大肠杆菌菌株中的每一个表达（a）能够合成6dEB类似物的修饰的红细菌6-脱氧红霉内酯B（6dEB）合成酶和/ 或（b）参与红霉素糖合成/附着/定制途径的基因以及参与合成一种或多种非红霉素糖部分如L-橄榄糖，L-夹竹桃糖，D-霉酚酸和吡咯硫胺的基因。

3. 发明申请

WO2010118093A3 SCREENING FOR ENVIRONMENTAL DNAS ENCODING ENZYMES FOR SYNTHESIZING TERPENOID-BASED THERAPEUTIC COMPOUNDS USING GENETICALLY MODIFIED E. COLI STRAINS 审中-公开
标题翻译：筛选用于合成基于TERPENOID的治疗化合物的环境DNA编码酶使用遗传修饰的大肠杆菌菌株
公开(公告)号：WO2010118093A3
公开(公告)日：2011-03-24
申请号：PCT/US2010030181
申请日：2010-04-07
申请人： UNIV TUFTS , PFEIFER BLAINE , WANG YONG
发明人： PFEIFER BLAINE , WANG YONG
IPC分类号： C12N15/70 , C12N1/21 , C12N15/52 , C12Q1/25 , C12Q1/68 , C12R1/19
CPC分类号： C12Q1/6888
摘要： A screening method for identifying microbial genes involved in biosynthesis of therapeutic terpenoid-based compounds using genetically modified E. coli strains that yield high levels of products of the non-mevalonate 1-deoxy-D-xylulose-5-phosphate pathway.
摘要翻译：使用产生高水平的非甲羟戊酸1-脱氧-D-木酮糖-5-磷酸途径的产物的遗传修饰的大肠杆菌菌株鉴定涉及治疗性萜类化合物的生物合成的微生物基因的筛选方法。

4. 发明申请

WO2012166408A3 GENETICALLY MODIFIED E. COLI STRAINS FOR PRODUCING ERYTHROMYCIN ANALOGS 审中-公开
标题翻译：用于生产红霉素类似物的遗传修饰的大肠杆菌菌株
公开(公告)号：WO2012166408A3
公开(公告)日：2013-01-24
申请号：PCT/US2012038780
申请日：2012-05-21
申请人： UNIV TUFTS , PFEIFER BLAINE , ZHANG HAORAN , PARK SUNG-HEE , JIANG MING
发明人： PFEIFER BLAINE , ZHANG HAORAN , PARK SUNG-HEE , JIANG MING
IPC分类号： C12N1/21 , C12N15/31 , C12N15/52 , C12P19/62
CPC分类号： C12N9/1029 , C12N15/52 , C12P19/62 , C12Y203/01094
摘要： Genetically engineered E. coli strains for producing analogs of erythromycin or 6-deoxyerythronolide B. Each of these E. coli strains expresses (a) a modified S. erythraea 6-deoxyerythronolide B (6dEB) synthase capable of synthesizing a 6dEB analog, and/or (b) genes involved in the erythromycin sugar synthesis/attachment/tailoring pathway and those involved in synthesis of one or more non-erythromycin sugar moieties, such as L-olivose, L-oleandrose, D-mycaminose, and pyrrolosamine.
摘要翻译：用于产生红霉素或6-脱氧红霉素B的类似物的遗传工程大肠杆菌菌株这些大肠杆菌菌株中的每一个表达（a）能够合成6dEB类似物的修饰的红细菌6-脱氧红霉内酯B（6dEB）合成酶和/ 或（b）参与红霉素糖合成/附着/定制途径的基因以及参与合成一种或多种非红霉素糖部分如L-橄榄糖，L-氯劳德糖，D-霉酚酸和吡咯硫胺的基因。

5. 发明申请

WO2002068613A1 BIOSYNTHESIS OF POLYKETIDE SYNTHASE SUBSTRATES 审中-公开
标题翻译：聚合酶基因的生物合成
公开(公告)号：WO2002068613A1
公开(公告)日：2002-09-06
申请号：PCT/US2002/006399
申请日：2002-02-28
申请人： THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY , KHOSLA, Chaitan , PFEIFER, Blaine
发明人： KHOSLA, Chaitan , PFEIFER, Blaine
IPC分类号： C12N1/21
CPC分类号： C12N9/1288 , C12N9/16 , C12N15/52 , C12P1/00 , C12P17/189
摘要： The use of enzymes which catalyze the production of starter and extender units for polyketides is described. In addition, modified loading modules are described, which can accept a variety of starting units such as substituted benzoates, and which can be used to generate substituted derivatives of natural products. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved in E. coli . Production can be enhanced in microbial organisms of polyketides and other secondary metabolites by delaying production until after exponential phase and by maintaining relatively constant nutrient levels in the medium. In addition, polyketide production can be enhanced by providing an expression system for a thioesterase II. Thus, by modifying the host organism and changing the culture conditions, synthesis of a secondary metabolite can be enhanced. The present invention also results in a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.
摘要翻译：描述了催化生产起始物的酶的使用和聚酮化合物的增量剂单元。此外，描述了可以接受多种起始单元如取代的苯甲酸酯并且可用于产生天然产物的取代衍生物的改性负载模块。这些酶可用于增强天然产生的聚酮化合物或合理设计的聚酮化合物的产率。通过使用这些技术，已经在大肠杆菌中完成了完整的聚酮化合物的合成。通过将生产延迟到指数期后并通过维持培养基中相对恒定的营养水平，可以增强聚酮化合物和其他次级代谢产物的微生物生物。此外，通过提供硫酯酶II的表达系统，可以增强聚酮化合物的产生。因此，通过改变宿主生物体并改变培养条件，可以增强次级代谢物的合成。本发明还导致具有所需特性的宿主生物体用于生产这种聚酮化合物并评估基因改组的结果。

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