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    • 2. 发明申请
    • METHOD OF PREPARATION OF A BIOSENSING SURFACE AND BIOSENSING SURFACE
    • 生物传感表面和生物活性表面的制备方法
    • WO2003052423A1
    • 2003-06-26
    • PCT/FI2002/001040
    • 2002-12-18
    • PELTONEN, JoukoIHALAINEN, Petri
    • PELTONEN, JoukoIHALAINEN, Petri
    • G01N33/553
    • B82Y30/00B05D1/185B05D1/202G01N33/54366G01N33/553
    • The present invention relates to a method of preparation of a biosensing surface where a host matrix comprising at least one analyte specific binding agent and at least one analytically inert matrix agent comprising binding units is dissolved to form a host matrix solution. The host matrix solution is applied onto a subphase surface, where it forms an oriented monolayer, which is compressed. A solid metal substrate is brought in contact with the monolayer on the subphase surface, and the monolayer is transferred onto the solid substrate by using hydrophobic interactions between the monolayer and the solid substrate. The monolayer is also bound covalently to the solid substrate via binding units comprised in the analytically inert matrix agent molecules of the host matrix. The invention relates also to a biosensing surface.
    • 本发明涉及一种制备生物传感表面的方法,其中将包含至少一种分析物特异性结合剂的宿主基质和至少一种包含结合单元的分析惰性基质剂溶解以形成宿主基质溶液。 将宿主基质溶液施加到亚相表面上,其中它形成定向单层,其被压缩。 使固体金属基底与亚相表面上的单层接触,并且通过使用单层和固体基底之间的疏水相互作用将单层转移到固体基底上。 单层也通过包含在宿主基质的分析惰性基质剂分子中的结合单元与固体基质共价结合。 本发明还涉及生物传感表面。