专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1997005280A1 DETECTION OF NUCLEIC ACIDS AND NUCLEIC ACID UNITS 审中-公开
标题翻译：检测核酸和核酸单位
公开(公告)号：WO1997005280A1
公开(公告)日：1997-02-13
申请号：PCT/GB1996001830
申请日：1996-07-25
申请人： UNIVERSITY OF STRATHCLYDE , GRAHAM, Duncan , LINACRE, Adrian, Matthew, Thornton , MUNRO, Calum, Hugh , SMITH, William, Ewan , WATSON, Nigel, Dean , WHITE, Peter, Cyril
发明人： UNIVERSITY OF STRATHCLYDE
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6816 , G01N21/658 , Y10T436/24 , C12Q2565/632
摘要： The invention relates to the detection of target nucleic acids or nucleic acid units in a sample, by obtaining a SER(R)S spectrum for a SER(R)S-active complex containing, or derived directly from, the target. The complex includes at least a SER(R)S-active label, and optionally a target binding species containing a nucleic acid or nucleic acid unit. In this detection method, the concentration of the target present in the SER(R)S-active complex, or of the nucleic acid or unit contained in the target binding species in the SER(R)S-active complex, is no higher than 10 moles per litre. Additionally or alternatively, one or more of the following features may be used with the method: i) the introduction of a polyamine; ii) modification of the target, and/or of the nucleic acid or nucleic acid unit contained in the target binding species, in a manner that promotes or facilitates its chemi-sorption onto a SER(R)S-active surface; iii) inclusion of a chemi-sorptive functional group in the SER(R)S-active label. The invention also provides SER(R)S-active complexes for use in such a method, a kit for use in carrying out the method or preparing the complexes and a method for sequencing a nucleic acid which comprises the use of the detection method to detect at least one target nucleotide or sequence of nucleotides within the acid.
摘要翻译：本发明涉及通过获得含有或直接衍生自靶的SER活性复合物的S（S）活性复合物，来检测样品中的靶核酸或核酸单元。所述复合物包括至少一种SER（R）S-活性标记，以及任选的含有核酸或核酸单元的靶结合物质。在该检测方法中，SER（R）S-活性复合物中存在的靶标或SER（S）活性复合物中目标结合物中所含的核酸或单元的浓度不高于 10〜10摩尔/升。另外或替代地，可以使用以下方法中的一个或多个以下特征：i）引入多胺; ii）以促进或促进其对SER活性表面的化学吸附的方式修饰靶，和/或包含在靶结合物种中的核酸或核酸单元; iii）在SER（R）S活性标签中包含化学吸附官能团。本发明还提供了用于这种方法的SER（R）S-活性复合物，用于实施该方法或制备复合物的试剂盒和核酸测序方法，其包括使用检测方法检测酸中的至少一个靶核苷酸或核苷酸序列。

IPRDB

热门服务

关于我们

友情链接

联系方式