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1. 发明申请

WO2011144345A1 REACTION VESSEL FOR PCR DEVICE AND METHOD OF PERFORMING PCR 审中-公开
标题翻译：用于PCR装置的反应容器和执行PCR的方法
公开(公告)号：WO2011144345A1
公开(公告)日：2011-11-24
申请号：PCT/EP2011/002507
申请日：2011-05-19
申请人： CURETIS AG , LÜDKE, Gerd , BOOS, Andreas , MOTEJADDED, Hassan , BACHER, Johannes
发明人： LÜDKE, Gerd , BOOS, Andreas , MOTEJADDED, Hassan , BACHER, Johannes
IPC分类号： B01L3/00 , B01L7/00 , C12Q1/68 , G01N33/543
CPC分类号： B01L7/52 , B01L3/502 , B01L2200/0631 , B01L2200/10 , B01L2300/047 , B01L2300/0636 , B01L2300/0681 , B01L2300/087 , B01L2400/0487 , B01L2400/049
摘要： The present invention provides a reaction vessel (20) for a PCR device. The reaction vessel (20) comprises a sample vial (32) defining a reaction chamber (33) for performing PCR and a storage vessel (62) defining a storage chamber (63) for optical detection. The reaction chamber (33) is in fluid communication with a liquid supply port (34) for supplying a liquid sample containing at least one target DNA to the reaction chamber (33). The reaction chamber (33) and the storage chamber (63) are in fluid communication via a spacer element (42) and a porous membrane (51) for hybridization of the at least one target DNA within the liquid sample onto specific immobilised hybridization probes. The lower end of the spacer element (42) extends into the reaction chamber (33), but does not reach the bottom thereof. The upper end of the spacer element (42) is located in proximity of the porous membrane (51), which is made from a material having different physical properties in a dry state and a wet state. In the dry state the porous membrane (51) allows air as well as liquid to pass therethrough. In the wet state the porous membrane (51) still allows the passage of liquid therethrough, but not of air, such that during a PCR the liquid sample remains in the reaction chamber (33) and after the PCR the reaction vessel (20) is configured to force the liquid sample via the spacer element (42) to the porous membrane (51 ) for hybridization and detection of the at least one target DNA in the liquid sample. Moreover, a PCR device comprising such a reaction vessel (20) as well as a method for performing PCR are described.
摘要翻译：本发明提供一种用于PCR装置的反应容器（20）。反应容器（20）包括限定用于进行PCR的反应室（33）的样品瓶（32）和限定用于光学检测的储存室（63）的储存容器（62）。反应室（33）与用于将含有至少一种目标DNA的液体样品供应到反应室（33）的液体供应口（34）流体连通。反应室（33）和储存室（63）经由间隔元件（42）和多孔膜（51）流体连通，用于将液体样品中的至少一个目标DNA杂交到特定的固定化杂交探针上。间隔元件（42）的下端延伸到反应室（33）中，但不到达其底部。间隔元件（42）的上端位于多孔膜（51）附近，该多孔膜由在干燥状态和湿态下具有不同物理性质的材料制成。在干燥状态下，多孔膜（51）允许空气以及液体通过。在湿状态下，多孔膜（51）仍然允许液体通过而不是空气通过，使得在PCR期间液体样品保留在反应室（33）中，并且在PCR之后，反应容器（20）是被配置为将液体样品经由间隔元件（42）强制到多孔膜（51），用于杂交和检测液体样品中的至少一种靶DNA。此外，描述了包含这种反应容器（20）的PCR装置以及用于进行PCR的方法。

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