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    • 1. 发明申请
    • SPHEROID TISSUE MICROARRAY AND METHODS OF MANUFACTURE
    • SPHEROID组织微阵列及其制造方法
    • WO2017174955A1
    • 2017-10-12
    • PCT/GB2016/053907
    • 2016-12-09
    • MICROMATRICES ASSOCIATES LTD
    • PLUMMER, Simon Matthew
    • C12N5/00C12M1/32
    • G01N1/36C12M21/08C12M23/04C12M23/12C12N5/0062C12N5/0068C12N5/0075C12N5/0619C12N5/0625C12N5/067C12N5/0693C12N2513/00C12N2533/76C12N2535/00G01N1/2806G01N1/312G01N2001/002G01N2001/362G01N2001/364G01N2001/366G01N2001/368
    • A spheroid tissue microarray comprises an array of tissue spheroids embedded within a porous mold. The product may be impregnated with a wax or resin and sectioned, and contains spheroids which are precisely located in a regular geometric grid. A method of manufacturing a spheroid tissue microarray comprises the steps of: forming a mold of porous material from liquid mold material in a casting mold, and allowing the liquid mold material to set; removing the porous mold from the casting mold; topping up the porous mold with further liquid mold material, and allowing recesses to form in the surface of the mold by the drawing-in of liquid mold material through shrinkage as the liquid mold material sets; placing tissue spheroids into the recesses in the surface of the porous mold; and sealing the tissue spheroids within the mold by topping off with liquid mold material and allowing the liquid mold material to set. An alternative method comprises the steps of: forming a mold of porous material from liquid mold material in a casting mold; allowing the liquid mold material to set; removing the porous mold from the casting mold; placing spheroids in recesses at the bases of wells in the mold of porous material; and sealing the spheroids within the porous mold by adding further porous material on top of the spheroids; wherein the recesses at the bases of the wells in the porous material are formed by protrusions of the casting mold carrying further, nipple-shaped, protrusions.
    • 球体组织微阵列包括嵌入多孔模具内的组织球体阵列。 该产品可能浸渍蜡或树脂和切片,并包含精确定位在一个规则的几何网格球体。 一种制造旋转椭圆形组织微阵列的方法,包括以下步骤:在铸模中由液态模具材料形成多孔材料模具,并且允许液态模具材料凝固; 从铸模中取出多孔模具; 用液态成型材料填充多孔模具,并通过在液态成型材料凝固时通过收缩使液态成型材料吸入而在模具表面形成凹槽; 将组织球体放入多孔模具表面的凹槽中; 通过用液体模具材料打顶并使液体模具材料固化来将组织球体密封在模具内。 一种替代方法包括以下步骤:在铸模中由液态成型材料形成多孔材料模具; 允许液态模具材料凝固; 从铸模中取出多孔模具; 将球状体放置在多孔材料模具中的孔底部的凹槽中; 通过在球体顶部添加更多孔材料将球体密封在多孔模具内; 其中在多孔材料的孔底部的凹槽由承载另外的乳头状突起的铸模的突起形成。
    • 2. 发明申请
    • A BIOREACTOR
    • 一个BIOREACTOR
    • WO2014102527A1
    • 2014-07-03
    • PCT/GB2013/051482
    • 2013-06-04
    • PLUMMER, Simon MatthewMICROMATRICES ASSOCIATES LTD
    • PLUMMER, Simon Matthew
    • C12M3/06C12M1/00
    • C12M29/18C12M23/16C12M23/58
    • A bioreactor (1) comprises one or more cell culture chambers (8) each containing an interchangeable scaffold (10) for the three dimensional culture of cells, one or more cell media chambers (2) for containing cell culture media, the cell media chamber (s) connected to the cell culture chamber (s) via one or more media flow channels (4, 47), and a pump (6) for pumping cell culture media through the media flow channel (s) from the cell media chamber (s) to the cell culture chamber (s). The bioreactor preferably comprises a nano wire- based detection system for the detection of target molecules using a measure of current/voltage changes in the nano wire. The bioreactor can preferably simultaneously measure several different biological indicator molecules, and enable molecular cellular responses to be monitored via simultaneous real-time measurement of multiple biological indicator molecules.
    • 生物反应器(1)包括一个或多个细胞培养室(8),每个细胞培养室包含用于细胞三维培养的可互换支架(10),用于容纳细胞培养基的一个或多个细胞培养基室(2),细胞培养室 通过一个或多个介质流动通道(4,47)连接到细胞培养室的泵(6)和用于通过来自细胞介质室的介质流动通道泵送细胞培养基的泵(6) s)到细胞培养室。 生物反应器优选地包括用于使用纳米线中的电流/电压变化的测量来检测靶分子的基于纳米线的检测系统。 生物反应器可以优选地同时测量几种不同的生物指示剂分子,并且通过多个生物指示剂分子的同时实时测量来实现分子细胞反应的监测。