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    • 1. 发明申请
    • BIOSENSOR AND METHOD OF MANUFACTURE
    • 生物传感器及其制造方法
    • WO2005066356A1
    • 2005-07-21
    • PCT/GB2004/005428
    • 2004-12-21
    • HYPOGUARD LIMITEDBUTTERS, Colin, W.HO, Wah, OnMAYNE, Christopher, J.RIPPETH, John, J.
    • BUTTERS, Colin, W.HO, Wah, OnMAYNE, Christopher, J.RIPPETH, John, J.
    • C12Q1/00
    • C12Q1/005C12Q1/001C12Q1/26
    • A non-mediated biosensor for indicating amperometrically thecatalytic activity of an oxidoreductase enzyme in the presence of a fluid containing a substance acted upon by said enzyme comprises : (a) a first substrate; (b) a working electrode and a reference electrode on the first substrate; (c) conductive tracks connected to said electrodes for making electrical connections with a test meter apparatus; (d) a second substrate overlying part of the first substrate; and (e) a spacer layer having a channel therein and disposed between the first substrate and the second substrate, the spacer layer channel co-operating with adjacent surfaces to define a capillary flow path which does not contain a mesh and which extends from an edge of at least one of said substrates to said electrodes. The working electrode includes : (f) an electrically-conductive base layer comprising particles of finely divided platinum-group metal or platinum-group metal oxide bonded together by a resin; (g) a top layer on the base layer, said top layer comprising a buffer; and (h) a catalytically-active quantity of said oxidoreductase enzyme in at least one of said base layer and said top layer.
    • 用于在含有由所述酶作用的物质的流体存在下指示电分析法的氧化还原酶的催化活性的非介导的生物传感器包括:(a)第一底物; (b)第一基板上的工作电极和参考电极; (c)连接到所述电极的导电轨道,用于与测试仪器设备进行电连接; (d)覆盖所述第一基板的一部分的第二基板; 和(e)间隔层,其中具有通道并设置在第一基板和第二基板之间,间隔层通道与相邻表面配合以限定毛细管流动路径,其不包含网状物并且从边缘 至少一个所述衬底到所述电极。 工作电极包括:(f)导电基层,其包含通过树脂粘合在一起的细碎的铂族金属或铂族金属氧化物的颗粒; (g)基层上的顶层,所述顶层包括缓冲液; 和(h)在所述基底层和所述顶层中的至少一个中的催化活性量的所述氧化还原酶。