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1. 发明申请

WO2011143044A2 A LYTIC REAGENT SYSTEM FOR FLOW CYTOMETRIC ANALYSIS OF LEUKOCYTES BASED ON MORPHOLOGY OR IMMUNOPHENOTYPES 审中-公开
标题翻译：基于形态学或免疫荧光的流式细胞仪分析淋巴细胞的LYTIC试剂系统
公开(公告)号：WO2011143044A2
公开(公告)日：2011-11-17
申请号：PCT/US2011035346
申请日：2011-05-05
申请人： IDEXX LAB INC , LIN JUIMING , DEKA CHIRANJIT
发明人： LIN JUIMING , DEKA CHIRANJIT
IPC分类号： C12Q1/00
CPC分类号： G01N33/537 , G01N33/56972
摘要： A lytic reagent composition and methods for differentiation and enumeration of leukocytes are disclosed. The lytic reagent is useful for rapid and automated sample preparation methods in an automated hematology analyzer, and also for manual and slow sample preparation methods required for immunophenotyping cells in a conventional fluorescence flow cytometer. The reagent system includes a nonionic surfactant in a hypotonic solution, an inorganic buffer to maintain the pH in a range of from about 6 to about 10, and optionally a leukocyte stabilizer. The reagent system is used to lyse red blood cells and stabilize the leukocytes so that antibody binding sites on the leukocytes remain unaffected by the process and the blood samples may be labeled with antibodies either before or after the lysis of the red blood cells.
摘要翻译：公开了一种裂解试剂组合物和白细胞的分化和计数方法。溶解试剂可用于自动血液分析仪中的快速和自动化样品制备方法，以及常规荧光流式细胞仪中免疫分型细胞所需的手动和慢速样品制备方法。试剂系统包括在低渗溶液中的非离子表面活性剂，将pH保持在约6至约10范围内的无机缓冲剂和任选的白细胞稳定剂。试剂系统用于裂解红细胞并稳定白细胞，使得白细胞上的抗体结合位点不受该过程的影响，血液样品可以在红细胞裂解之前或之后用抗体标记。

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