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1. 发明申请

WO2018218033A3 USE OF AN ESTERASE TO ENHANCE ETHYL ESTER CONTENT IN FERMENTATION MEDIA 审中-公开
公开(公告)号：WO2018218033A3
公开(公告)日：2018-11-29
申请号：PCT/US2018/034410
申请日：2018-05-24
申请人： MCCURDY, Alexander T. , BLY, Steven T. , LEWIS, Stephen M. , GACKE, Benjamin P. , BREITLING, Brandon James
发明人： MCCURDY, Alexander T. , BLY, Steven T. , LEWIS, Stephen M. , GACKE, Benjamin P. , BREITLING, Brandon James
IPC分类号： C12P7/64 , C12P7/62 , C12P7/06
摘要： Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.

2. 发明申请

WO2012122393A3 SYSTEMS AND METHODS FOR IMPROVING STILLAGE 审中-公开
标题翻译：用于改善静止的系统和方法
公开(公告)号：WO2012122393A3
公开(公告)日：2014-04-24
申请号：PCT/US2012028311
申请日：2012-03-08
申请人： POET RES INC , TEWALT JACOB P , LEWIS STEPHEN M , BOOTSMA JASON A
发明人： TEWALT JACOB P , LEWIS STEPHEN M , BOOTSMA JASON A
IPC分类号： F25B49/04 , F25B15/00 , F25B49/00
CPC分类号： C12P1/02 , C12M21/12 , C12M23/58 , C12M47/14 , C12P7/06 , Y02E50/17
摘要： Systems and methods for improving stillage are disclosed. Stillage may include either whole stillage or thin stillage. The system includes taking the stillage and placing it within a bioreactor with an inoculation of fungi. The fungi may include any of Aspergillus niger, Phanerochaete chrysosporium and Yarrowia lipolytica. The fungi and stillage broth is then subjected to fermentation which removes solubles and particulates from the stillage. The fungi generate a biomass material that may be collected and dried for use as a nutritional supplement or other purpose. The remaining liquid is a clarified, treated stillage suitable for a variety of downstream applications, including being used as a backset in an ethanol production facility.
摘要翻译：公开了用于改善釜渣的系统和方法。静物可以包括整个酒糟或薄的酒糟。该系统包括服用酒糟并将其置于生物反应器中并接种真菌。真菌可以包括黑曲霉，Phanerochaete chrysosporium和解脂耶氏酵母中的任何一种。然后将真菌和淀粉肉汤进行发酵，从釜中除去可溶物和微粒。真菌产生可以收集和干燥以用作营养补充剂或其它目的的生物质材料。剩余的液体是适用于各种下游应用的澄清处理过的废液，包括用作乙醇生产设备的回填装置。

3. 发明申请

WO2012078885A3 SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT 审中-公开
标题翻译：用于饲料质量评估的系统和方法
公开(公告)号：WO2012078885A3
公开(公告)日：2013-01-24
申请号：PCT/US2011063963
申请日：2011-12-08
申请人： POET RES INC , LEWIS STEPHEN M , NARENDRANATH NEELAKANTAM V
发明人： LEWIS STEPHEN M , NARENDRANATH NEELAKANTAM V
IPC分类号： C12Q1/44 , G01N33/10
CPC分类号： C12Q1/44 , G01N33/10 , G01N2333/415
摘要： Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample.
摘要翻译：提供原料质量评估，可用于确定饲料质量（如玉米粒）。该评估确定原料内的内源酶活性，其与生淀粉水解（未煮熟）体系中的总乙醇产量相关。在一些实施方案中，提供含有面粉（研磨原料）的样品。在某些情况下，将面粉稀释在水中并用磷酸盐缓冲溶液缓冲。缓冲的面粉溶液与分子或荧光染料接触，例如以可检测的方式通过酶（酯酶）活性改变的荧光素二乙酸酯和/或二氟荧光素。使用培养箱可以促进酶活性。可以用荧光计测量可检测的变化。在一些实施方案中，可以进行两次孵育和荧光测量。通过减去测量的荧光之间的差异，可以发现与原料样品中的内源性酶活性相关的荧光指数。

4. 发明申请

WO2005099476A1 IMPROVED DISTILLATION PROCESS 审中-公开
标题翻译：改进的蒸馏工艺
公开(公告)号：WO2005099476A1
公开(公告)日：2005-10-27
申请号：PCT/US2005/011428
申请日：2005-04-06
申请人： NOVOZYMES NORTH AMERICA, INC. , BROIN AND ASSOCIATES, INC. , WENGER, Kevin S. , ALLAIN, Eric , LEWIS, Stephen M. , FINCK, John Michael , ROTH, Debbie Lynn
发明人： WENGER, Kevin S. , ALLAIN, Eric , LEWIS, Stephen M. , FINCK, John Michael , ROTH, Debbie Lynn
IPC分类号： A23J1/12
CPC分类号： B01D3/001 , B01D3/34 , C12P7/06 , Y02E50/16 , Y02E50/17
摘要： The present invention relates to an improved process of distilling fermented mash, wherein one or more amylases and/or proteases are added to the fermentation mash before or during distillation.
摘要翻译：本发明涉及一种蒸馏发酵的糊状物的改进方法，其中在蒸馏之前或期间将一种或多种淀粉酶和/或蛋白酶加入到发酵醪液中。

5. 发明申请

WO2018218033A2 USE OF AN ESTERASE TO ENHANCE ETHYL ESTER CONTENT IN FERMENTATION MEDIA 审中-公开
公开(公告)号：WO2018218033A2
公开(公告)日：2018-11-29
申请号：PCT/US2018/034410
申请日：2018-05-24
申请人： MCCURDY, Alexander T. , BLY, Steven T. , LEWIS, Stephen M. , GACKE, Benjamin P. , BREITLING, Brandon James
发明人： MCCURDY, Alexander T. , BLY, Steven T. , LEWIS, Stephen M. , GACKE, Benjamin P. , BREITLING, Brandon James
IPC分类号： C12P7/64 , C12P7/62 , C12P7/06
摘要： Methods to prepare vegetable oil compositions having an elevated ethyl ester content are provided.

6. 发明申请

WO2012078885A2 SYSTEMS AND METHODS FOR FEEDSTOCK QUALITY ASSESSMENT 审中-公开
标题翻译：用于饲料质量评估的系统和方法
公开(公告)号：WO2012078885A2
公开(公告)日：2012-06-14
申请号：PCT/US2011/063963
申请日：2011-12-08
申请人： POET RESEARCH, INC. , LEWIS, Stephen M. , NARENDRANATH, Neelakantam V.
发明人： LEWIS, Stephen M. , NARENDRANATH, Neelakantam V.
IPC分类号： C12Q1/44
CPC分类号： C12Q1/44 , G01N33/10 , G01N2333/415
摘要： Assessing quality of feedstock is provided and may be useful for determining quality of feed (such as corn kernels). The assessment determines endogenous enzyme activity within the feedstock, which correlates with total ethanol yields in raw starch hydrolysis (non-cooked) systems. In some embodiments, a sample containing flour (ground feedstock) is provided. In some cases, flour is diluted in water and buffered with a phosphate buffered solution. The buffered flour solution is contacted with a molecule, or fluorescent dye, such as fluorescein diacetate and/or difluorofluorescein that is altered by enzymatic (esterase) activity in a detectable fashion. Enzyme activity may be facilitated using an incubator. The detectible alteration may be measured using a fluorometer. In some embodiments, two incubations and fluorescence measurements can be performed. By subtracting the difference between the measured fluorescence, a fluorescence index may be found, which correlates with the endogenous enzyme activity in the feedstock sample.
摘要翻译：提供原料质量评估，可用于确定饲料质量（如玉米粒）。该评估确定原料内的内源酶活性，其与生淀粉水解（未煮熟）体系中的总乙醇产量相关。在一些实施方案中，提供含有面粉（研磨原料）的样品。在某些情况下，将面粉稀释在水中并用磷酸盐缓冲溶液缓冲。缓冲的面粉溶液与分子或荧光染料接触，例如以可检测的方式通过酶（酯酶）活性改变的荧光素二乙酸酯和/或二氟荧光素。使用培养箱可以促进酶活性。可以用荧光计测量可检测的变化。在一些实施方案中，可以进行两次孵育和荧光测量。通过减去测量的荧光之间的差异，可以发现与原料样品中的内源性酶活性相关的荧光指数。

7. 发明申请

WO2012122393A2 SYSTEMS AND METHODS FOR IMPROVING STILLAGE 审中-公开
标题翻译：用于改善静止的系统和方法
公开(公告)号：WO2012122393A2
公开(公告)日：2012-09-13
申请号：PCT/US2012/028311
申请日：2012-03-08
申请人： POET RESEARCH, INC. , TEWALT, Jacob P. , LEWIS, Stephen M. , BOOTSMA, Jason A.
发明人： TEWALT, Jacob P. , LEWIS, Stephen M. , BOOTSMA, Jason A.
IPC分类号： F25B49/04 , F25B49/00 , F25B15/00
CPC分类号： C12P1/02 , C12M21/12 , C12M23/58 , C12M47/14 , C12P7/06 , Y02E50/17
摘要： Systems and methods for improving stillage are disclosed. Stillage may include either whole stillage or thin stillage. The system includes taking the stillage and placing it within a bioreactor with an inoculation of fungi. The fungi may include any of Aspergillus niger, Phanerochaete chrysosporium and Yarrowia lipolytica . The fungi and stillage broth is then subjected to fermentation which removes solubles and particulates from the stillage. The fungi generate a biomass material that may be collected and dried for use as a nutritional supplement or other purpose. The remaining liquid is a clarified, treated stillage suitable for a variety of downstream applications, including being used as a backset in an ethanol production facility.
摘要翻译：公开了用于改善釜渣的系统和方法。静物可以包括整个酒糟或薄的酒糟。该系统包括服用酒糟并将其置于生物反应器中并接种真菌。真菌可以包括黑曲霉，Phanerochaete chrysosporium和解脂耶氏酵母中的任何一种。然后将真菌和淀粉肉汤进行发酵，从釜中除去可溶物和微粒。真菌产生可以收集和干燥以用作营养补充剂或其它目的的生物质材料。剩余的液体是适用于各种下游应用的澄清处理过的废液，包括用作乙醇生产设备的回填装置。

8. 发明申请

WO2012125739A1 SYSTEMS AND METHODS FOR IMPROVING ETHANOL YIELD 审中-公开
标题翻译：改善乙醇含量的系统和方法
公开(公告)号：WO2012125739A1
公开(公告)日：2012-09-20
申请号：PCT/US2012/029093
申请日：2012-03-14
申请人： POET RESEARCH, INC. , LEWIS, Stephen M. , NARENDRANATH, Neelakantam V.
发明人： LEWIS, Stephen M. , NARENDRANATH, Neelakantam V.
IPC分类号： C12P7/06
CPC分类号： C12P7/08 , C12P7/06 , Y02E50/17
摘要： Systems and methods for improving ethanol yield are provided. A feedstock is ground suitably fine for use in fermentation. The feedstock may include corn or any other suitable material. In some cases, the feedstock undergoes a fractionation prior to grinding. The ground feedstock may be slurried with water and enzymes to facilitate conversion of the starch in the feedstock to sugars. The slurry may be about 35% solids. After being slurried, an ethanologen may be added. Additionally, the pH of the slurry may be adjusted to between 4.2 and 5.2 to facilitate the priming. A primer is added to the slurry. The primer may include any weak acid, and in some embodiments includes acetic acid. Acetic acid, when used as a primer, may be added at a concentration of between 1200 and 3600 parts per million. The slurry is fermented to produce improved yields of ethanol.
摘要翻译：提供了提高乙醇产量的系统和方法。将原料粉碎成适用于发酵的原料。原料可以包括玉米或任何其它合适的材料。在一些情况下，原料在研磨之前进行分馏。地面原料可以用水和酶进行浆化，以促进原料中的淀粉转化为糖。浆料可以是约35％的固体。在酝酿之后，可能会添加一个种族主义者。此外，浆料的pH可以调节到4.2和5.2之间以便于起动。向浆料中加入底漆。引物可以包括任何弱酸，并且在一些实施方案中包括乙酸。当用作底漆时，乙酸可以以1200至3600ppm的浓度加入。将浆料发酵以产生改善的乙醇产率。

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