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1. 发明申请

WO1996030520A2 SYNTHETIC HPV6/11 HYBRID L1 DNA ENCODING HUMAN PAPILLOMAVIRUS TYPE 11 L1 PROTEIN 审中-公开
标题翻译：合成HPV6 / 11混合L1编码人类斑马鱼11型L1蛋白
公开(公告)号：WO1996030520A2
公开(公告)日：1996-10-03
申请号：PCT/US1996004117
申请日：1996-03-26
申请人： MERCK & CO., INC. , HOFMANN, Kathryn, J. , JANSEN, Kathrin, U. , NEEPER, Michael, P. , JOYCE, Joseph, G. , GEORGE, Hugh, A. , LEHMAN, E., Dale
发明人： MERCK & CO., INC.
IPC分类号： C12N15/37
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/51 , C12N2710/20022
摘要： The present invention is directed to a synthetic DNA molecule encoding purified human papillomavirus type 11 L1 protein and derivatives thereof.
摘要翻译：本发明涉及编码纯化的人乳头瘤病毒11型L1蛋白及其衍生物的合成DNA分子。

2. 发明申请

WO9630520A3 SYNTHETIC HPV6/11 HYBRID L1 DNA ENCODING HUMAN PAPILLOMAVIRUS TYPE 11 L1 PROTEIN 审中-公开
标题翻译：合成HPV6 / 11混合L1编码人类斑马鱼11型L1蛋白
公开(公告)号：WO9630520A3
公开(公告)日：1996-11-07
申请号：PCT/US9604117
申请日：1996-03-26
申请人： MERCK & CO INC , HOFMANN KATHRYN J , JANSEN KATHRIN U , NEEPER MICHAEL P , JOYCE JOSEPH G , GEORGE HUGH A , LEHMAN E DALE
发明人： HOFMANN KATHRYN J , JANSEN KATHRIN U , NEEPER MICHAEL P , JOYCE JOSEPH G , GEORGE HUGH A , LEHMAN E DALE
IPC分类号： C12N15/09 , A61K39/00 , A61K39/12 , A61P31/12 , C07K14/025 , C07K16/08 , C12N15/37 , C12P21/02 , C12P21/08 , C12R1/645 , A61K46/00 , C12N7/00 , C12N15/81
CPC分类号： C07K14/005 , A61K39/00 , A61K2039/51 , C12N2710/20022
摘要： The present invention is directed to a synthetic DNA molecule encoding purified human papillomavirus type 11 L1 protein and derivatives thereof.

3. 发明申请

WO1993025229A1 A METHOD FOR PURIFYING RECOMBINANT TAP 审中-公开
标题翻译：一种用于纯化重组蛋白TAP的方法
公开(公告)号：WO1993025229A1
公开(公告)日：1993-12-23
申请号：PCT/US1993005283
申请日：1993-06-02
申请人： MERCK & CO., INC. , LEHMAN, E., Dale , FREYMEYER, Daniel
发明人： MERCK & CO., INC.
IPC分类号： A61K37/64
CPC分类号： C07K14/811
摘要： The invention is a method for purifying recombinantly produced TAP, a 60 amino acid anticoagulant protein derived from ticks. S. cerevisiae ATCC 20984 yeast cells are cultured in yeast cell culture media suitable for expressing TAP. The cells are separated from expressed protein by crossflow ultrafiltration and diafiltration to clarify the broth. Preferably, pH is thereafter adjusted to about 3.5. The TAP is then concentrated and partially purified by adsorption on to a POROS cation-exchange resin, preferably a POROS II HS/P sulfopropyl cation-exchange resin. The broth is preferably pumped onto the resin using a peristaltic pump. The protein is eluted from the resin with a concentrated salt buffer, preferably 1 M NaCl. The TAP in the eluate is then purified to greater than 96 % homogeneity with a step gradient generated with a preparative HPLC pump. The protein is then desalted and concentrated on a reverse phase chromatography column, preferably a POROS R/M reverse phase chromatography column, and thereafter lyophilized.
摘要翻译：本发明是用于纯化重组产生的TAP的方法，该TAP是来源于蜱的60个氨基酸的抗凝血蛋白。酿酒酵母ATCC 20984酵母细胞在适于表达TAP的酵母细胞培养基中培养。通过交叉流超滤和渗滤将细胞与表达的蛋白质分离，以澄清肉汤。优选地，此后将pH调节至约3.5。然后将TAP浓缩并通过吸附到POROS阳离子交换树脂，优选POROS II HS / P磺丙基阳离子交换树脂上部分纯化。优选使用蠕动泵将肉汤泵送到树脂上。蛋白质用浓盐酸缓冲液，优选1M NaCl从树脂中洗脱出来。然后用制备型HPLC泵产生的梯度梯度将洗脱液中的TAP纯化至大于96％的均匀性。然后将蛋白质脱盐并浓缩在反相色谱柱，优选POROS R / M反相色谱柱上，然后冻干。

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