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    • 9. 发明申请
    • ENZYMATIC REVERSE HYDROLYSIS OF HYDROPHILIC SUBSTRATES - PREPARATION OF AMPHIPHILIC COMPOUNDS
    • 水解底物的酶反向水解 - 大分子化合物的制备
    • WO1992014830A1
    • 1992-09-03
    • PCT/EP1992000307
    • 1992-02-12
    • SCHNEIDER, Manfred, P.LAUMEN, Kurt, E.BERGER, Matthias
    • C12P01/00
    • C12P13/001C12P1/00C12P7/62C12P7/6436C12P7/6454C12P7/6481C12P9/00C12P13/00C12P13/02C12P13/04C12P19/00C12P19/02C12P19/44C12P21/02
    • The invention relates to methods for the production of amphiphilic products such as esters, sugar-esters, peptide-esters, glycolipids, glycoproteins, lipoproteins, peptides, and phosphates of alcohols, sugars, and nucleosides. The methods promote enzymatically catalyzed reactions between hydrophilic substrates such as glucerol, glucose, amino acids, and nucleosides, and second substrates such as free fatty acids, triglycerides, vinylesters, amino acids, and phosphates. The method is also applied to enzymatic reactions with saccharides and polyalcohols. The hydrophilic substrates are adsorbed to finely divided solid supports such as silica gel, diatomaceous earths, or activated chracoals in order to promote the dispersion of the hydrophilic substrates within hydrophobic substrates and solvents. Hydrophobic solvents such as n-hexane and t-butylmethylether may be included in the reaction mixtures. Reactions are conducted under non-aqueous conditions in order to promote reverse hydrolysis. Methods are provided for the production of isomerically pure 1,3-diglycerides. Further methods are disclosed for the production and specific precipitation of pure 1-monogycerides through the use of a reactor/separator system. Enzymes used in the methods include lipases from M. mihei and P. fluorescens, glycosidases such as beta - galactosidase, proteases such as chymotrypsin, and acid or alkaline phosphatases. Compositions are provided comprising alcohols, carbohydrates, amino acids, or peptides adsorbed onto solid supports such as silica gel.
    • 本发明涉及制备醇,糖和核苷类的两亲产物如酯,糖酯,肽 - 酯,糖脂,糖蛋白,脂蛋白,肽和磷酸酯的方法。 这些方法促进亲水底物如葡萄糖,葡萄糖,氨基酸和核苷之间的酶催化反应,以及第二底物如游离脂肪酸,甘油三酸酯,乙烯基酯,氨基酸和磷酸酯。 该方法也适用于与糖和多元醇的酶反应。 为了促进亲水性底物在疏水性底物和溶剂中的分散,亲水性底物被吸附到细分散的固体载体如硅胶,硅藻土或活化的色母体上。 疏水性溶剂如正己烷和叔丁基甲基醚可以包括在反应混合物中。 反应在非水性条件下进行,以促进反向水解。 提供了用于生产异构纯的1,3-甘油二酯的方法。 公开了通过使用反应器/分离器系统来生产和特异性沉淀纯的1-单加氧化物的其它方法。 用于该方法的酶包括来自M.mehei和荧光假单胞菌的脂肪酶,糖苷酶如β-半乳糖苷酶,蛋白酶如胰凝乳蛋白酶和酸或碱性磷酸酶。 提供了包含醇,碳水化合物,氨基酸或吸附在固体支持物例如硅胶上的肽的组合物。