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1. 发明申请

WO2010114727A1 MICROORGANISM CONCENTRATION PROCESS AND DEVICE 审中-公开
标题翻译：微生物浓缩过程和装置
公开(公告)号：WO2010114727A1
公开(公告)日：2010-10-07
申请号：PCT/US2010/028122
申请日：2010-03-22
申请人： 3M INNOVATIVE PROPERTIES COMPANY , KSHIRSAGAR, Manjiri, T. , RABINS, Andrew, W.
发明人： KSHIRSAGAR, Manjiri, T. , RABINS, Andrew, W.
IPC分类号： C12M1/12 , B01D39/14 , B01D37/00 , B01D69/02
CPC分类号： C08J9/0066 , B01D61/147 , B01D63/00 , C08J9/0061 , C08J2323/06 , C08J2423/06 , C12Q1/24
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises an amorphous metal silicate and that has a surface composition having a metal atom to silicon atom ratio of less than or equal to 0.5, as determined by X-ray photoelectron spectroscopy (XPS); (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device.
摘要翻译：用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含烧结的多孔聚合物基质的浓缩装置，所述多孔聚合物基质包含至少一种浓缩剂，所述浓缩剂包含无定形金属硅酸盐，并且具有具有金属原子与硅原子的表面组成比例小于或等于0.5，如通过X射线光电子能谱（XPS）测定的; （b）提供包含至少一种微生物菌株的样品; 和（c）使浓缩装置与样品接触，使得至少一部分微生物菌株与浓缩装置结合或捕获。

2. 发明申请

WO2010078284A1 COLIFORM DETECTION PROCESS AND KIT FOR USE THEREIN 审中-公开
标题翻译：检测过程及其使用的工具包
公开(公告)号：WO2010078284A1
公开(公告)日：2010-07-08
申请号：PCT/US2009/069629
申请日：2009-12-29
申请人： 3M INNOVATIVE PROPERTIES COMPANY , KSHIRSAGAR, Manjiri, T.
发明人： KSHIRSAGAR, Manjiri, T.
IPC分类号： C12Q1/10 , C12Q1/04 , G01N1/40 , C12N11/14 , C12Q1/24
CPC分类号： C12Q1/04 , C12Q1/10 , C12Q1/24 , G01N33/56916 , G01N2520/00
摘要： A process comprises (a) providing (1) at least one sample suspected of comprising at least one coliform strain, (2) at least one culture device comprising at least one culture medium that is hydrated or hydratable, and (3) at least one particulate concentration agent that is substantially optically transparent when in contact with the culture medium in the culture device when the culture medium is hydrated; (b) placing the particulate concentration agent in contact with the sample such that at least a portion of the coliform strain is bound to the particulate concentration agent to form coliform-bound particulate concentration agent; (c) placing the coliform-bound particulate concentration agent in contact with the culture medium; (d) incubating the culture device comprising the coliform-bound particulate concentration agent in contact with the culture medium, the culture medium being hydrated; and (e) optically detecting the presence of the coliform strain without separating the coliform strain from the particulate concentration agent.
摘要翻译：一种方法包括（a）提供（1）至少一种怀疑含有至少一种大肠杆菌菌株的样品，（2）至少一种包含至少一种水合或可水合的培养基的培养装置，和（3）至少一种当培养基水合时，当与培养装置中的培养基接触时基本上是光学透明的颗粒浓缩剂; （b）使颗粒浓缩剂与样品接触，使得至少一部分大肠杆菌菌株与颗粒物浓缩剂结合形成大肠菌群结合的颗粒浓缩剂; （c）使大肠杆菌结合的颗粒浓缩剂与培养基接触; （d）将包含大肠杆菌结合的颗粒浓缩剂的培养装置与培养基接触培养，培养基水合; 和（e）光学检测大肠菌群的存在，而不从大颗粒浓缩剂中分离大肠杆菌菌株。

3. 发明申请

WO2009046081A3 PROCESS FOR LIMITING THE GROWTH OF MICROORGANISMS 审中-公开
公开(公告)号：WO2009046081A3
公开(公告)日：2009-04-09
申请号：PCT/US2008/078413
申请日：2008-10-01
申请人： 3M INNOVATIVE PROPERTIES COMPANY , KSHIRSAGAR, Manjiri T. , KSHIRSAGAR, Tushar A. , WOOD, Thomas E.
发明人： KSHIRSAGAR, Manjiri T. , KSHIRSAGAR, Tushar A. , WOOD, Thomas E.
IPC分类号： A01N59/16 , B01J23/52 , B01J23/66 , B01J35/00 , B01J37/34 , A01P1/00 , C23C14/00
摘要： A process for limiting the growth of microorganisms comprises (a) providing an antimicrobial agent comprising fine-nanoscale gold on a support medium comprising nanoparticulate titania, the fine-nanoscale gold having been deposited on the support medium by physical vapor deposition; and (b) contacting at least one microorganism with the antimicrobial agent.

4. 发明申请

WO2012078426A2 MICROORGANISM CONCENTRATION PROCESS AND DEVICE 审中-公开
标题翻译：微生物浓缩过程和装置
公开(公告)号：WO2012078426A2
公开(公告)日：2012-06-14
申请号：PCT/US2011/062618
申请日：2011-11-30
申请人： 3M INNOVATIVE PROPERTIES COMPANY , KSHIRSAGAR, Manjiri T. , RABINS, Andrew W.
发明人： KSHIRSAGAR, Manjiri T. , RABINS, Andrew W.
IPC分类号： C12Q1/02 , C12Q1/06 , C12M1/16 , C12M1/34
CPC分类号： G01N1/405 , C12M47/02 , C12Q1/04 , C12Q1/24 , G01N1/40 , G01N33/84
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.
摘要翻译：用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供浓缩装置，其包括（1）多孔纤维非织造基质和（2）至少一种包含金属硅酸盐的浓缩剂的颗粒，所述颗粒位于多孔纤维非织造基体中; （b）提供包含至少一种靶细胞分析物的样品; （c）使浓缩装置与样品接触，使得至少一部分目标细胞分析物与浓缩装置结合或捕获; 和（d）检测至少一种结合的靶细胞分析物的存在。

5. 发明申请

WO2009102824A1 METHODS AND COMPOSITIONS FOR DETECTING MICROORGANISMS 审中-公开
标题翻译：检测微生物的方法和组合物
公开(公告)号：WO2009102824A1
公开(公告)日：2009-08-20
申请号：PCT/US2009/033847
申请日：2009-02-12
申请人： 3M INNOVATIVE PROPERTIES COMPANY , ROSCOE, Stephen B. , MOELLER, Stephanie J. , KSHIRSAGAR, Manjiri T.
发明人： ROSCOE, Stephen B. , MOELLER, Stephanie J. , KSHIRSAGAR, Manjiri T.
IPC分类号： G01N33/53
CPC分类号： G01N33/569 , G01N33/5308 , G01N2333/39 , G01N2400/24 , G01N2469/10
摘要： Methods of analyzing a sample for target microorganisms of interest are described. In particular, the methods are useful for detecting a variety of yeast and mold microorganisms based on the presence of a ubiquitous cell wall component, such as zymosan. Methods of analyzing a sample for fungal microorganisms using liposomes and/or culture media are also described.
摘要翻译：描述了目标微生物样品分析方法。特别地，该方法可用于基于普遍存在的细胞壁组分（例如酵母聚糖）的存在来检测各种酵母和霉菌微生物。还描述了使用脂质体和/或培养基分析真菌微生物样品的方法。

6. 发明申请

WO2010078482A1 LIVE BIOLOAD DETECTION USING MICROPARTICLES 审中-公开
标题翻译：使用微生物进行实时荧光检测
公开(公告)号：WO2010078482A1
公开(公告)日：2010-07-08
申请号：PCT/US2009/069898
申请日：2009-12-31
申请人： 3M INNOVATIVE PROPERTIES COMPANY , RAJAGOPAL, Raj , HALVERSON, Kurt, J. , KSHIRSAGAR, Manjiri, T. , AYSTA, James, E.
发明人： RAJAGOPAL, Raj , HALVERSON, Kurt, J. , KSHIRSAGAR, Manjiri, T. , AYSTA, James, E.
IPC分类号： B01L3/00 , C12M1/12 , C12Q1/68 , G01N33/53 , G01N33/543 , G01N33/545
CPC分类号： C12Q1/04 , A61B10/0045 , A61B10/02 , B01L3/5082 , B01L2200/026 , B01L2200/10 , C12M23/08 , C12M23/32 , C12M23/34 , C12M33/02 , G01N33/56916 , G01N2333/245
摘要： The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods.
摘要翻译：本发明提供了将细胞浓缩到微粒上，浓缩微粒并检测细胞的方法。本发明还包括根据该方法使用的单一样品制备和检测装置。

7. 发明申请

WO2010078399A2 SAMPLING DEVICES AND METHODS FOR CONCENTRATING MICROORGANISMS 审中-公开
标题翻译：采样装置和浓缩微生物的方法
公开(公告)号：WO2010078399A2
公开(公告)日：2010-07-08
申请号：PCT/US2009069780
申请日：2009-12-30
申请人： 3M INNOVATIVE PROPERTIES CO , KSHIRSAGAR MANJIRI T , HALVERSON KURT J , PERCY NEIL , AYSTA JAMES E
发明人： KSHIRSAGAR MANJIRI T , HALVERSON KURT J , PERCY NEIL , AYSTA JAMES E
IPC分类号： C12M1/26
CPC分类号： C12Q1/24 , C12M47/02 , C12M47/04 , G01N1/40 , G01N1/405 , G01N2001/4088
摘要： The present disclosure describes methods for concentrating microorganisms with concentration agents in a sampling device and the sampling device described herein. More specifically, methods for concentrating microorganisms from large volume samples with concentration agents in a sampling device can provide for rapid, low cost, simple (involving no complex equipment or procedures), and/or effective processes under a variety of conditions.
摘要翻译：本公开描述了将浓缩剂浓缩在采样装置中的微生物和本文所述的采样装置的方法。更具体地说，用于将来自大容量样品的微生物浓缩在采样装置中的浓缩剂的方法可以在各种条件下提供快速，低成本，简单（不涉及复杂的设备或程序）和/或有效过程。

8. 发明申请

WO2009046183A1 MICROORGANISM CONCENTRATION PROCESS 审中-公开
标题翻译：微生物浓缩过程
公开(公告)号：WO2009046183A1
公开(公告)日：2009-04-09
申请号：PCT/US2008/078563
申请日：2008-10-02
申请人： 3M INNOVATIVE PROPERTIES COMPANY , KSHIRSAGAR, Manjiri T. , KSHIRSAGAR, Tushar A. , WOOD, Thomas E.
发明人： KSHIRSAGAR, Manjiri T. , KSHIRSAGAR, Tushar A. , WOOD, Thomas E.
IPC分类号： C12N1/02 , G01N1/40 , C12N11/14 , C12Q1/04 , C12Q1/06 , C12Q1/14 , C12Q1/24 , B01J20/02 , B01J20/06 , B01J20/14
CPC分类号： C12N1/02 , B01J20/0229 , B01J20/06 , B01J20/28004 , B01J20/2803 , C12N11/14 , C12Q1/04 , C12Q1/24 , G01N1/405 , G01N33/54313
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a particulate concentration agent that comprises gamma-FeO(OH); (b) providing a fluid sample comprising at least one microorganism strain; and (c) contacting the concentration agent with the sample such that at least a portion of the concentration agent is dispersed in the sample and at least a portion of the at least one microorganism strain is bound to or captured by the concentration agent.
摘要翻译：用于捕获或浓缩用于检测或测定的微生物的方法包括（a）提供包含γ-FeO（OH）的颗粒浓缩剂; （b）提供包含至少一种微生物菌株的流体样品; 和（c）使浓缩剂与样品接触，使得至少一部分浓缩剂分散在样品中，并且至少一部分微生物菌株与浓缩剂结合或捕获。

9. 发明申请

WO2008134472A1 COMPOSITIONS, METHODS, AND DEVICES FOR ISOLATING BIOLOGICAL MATERIALS 审中-公开
标题翻译：用于分离生物材料的组合物，方法和装置
公开(公告)号：WO2008134472A1
公开(公告)日：2008-11-06
申请号：PCT/US2008/061508
申请日：2008-04-25
申请人： 3M INNOVATIVE PROPERTIES COMPANY , XIA, Wensheng , HOLT, Paul N. , PARTHASARATHY, Ranjani V. , KSHIRSAGAR, Manjiri T.
发明人： XIA, Wensheng , HOLT, Paul N. , PARTHASARATHY, Ranjani V. , KSHIRSAGAR, Manjiri T.
IPC分类号： C12N15/10 , C12Q1/24 , C12Q1/68
CPC分类号： C12N15/1006 , G01N33/569
摘要： Compositions, methods, devices, and kits, which include an immobilized-metal support material comprising a substrate having a plurality of -C(O)O - or -P(O)(-OH) 2-x (-O - ) x groups bound to the substrate and a plurality of metal ions, M y+ , bound to the -C(O)O - or -P(O)(-OH) 2-x (-O - ) x groups; wherein M is selected from the group consisting of zirconium, gallium, iron, aluminum, scandium, titanium, vanadium, yttrium, and a lanthanide; y is an integer from 3 to 6; and x is 1 or 2, and to which microorganisms and polynucleotides bind, and which can be used for separating and optionally assaying microorganisms and/or a polynucleotide from a sample material are disclosed.
摘要翻译：组合物，方法，装置和试剂盒，其包括固定金属载体材料，其包含具有多个-C（O）O - 或-P（O）（ - OH） > - >结合到衬底上的多个金属离子，M + Y +， - ， - 与-C（O）O - 或 - （O）（ - OH）2-x（-O - ） - SUB> x 组; 其中M选自锆，镓，铁，铝，钪，钛，钒，钇和镧系元素; y为3〜6的整数; 并且x为1或2，并且公开了微生物和多核苷酸结合，并且其可用于从样品材料分离和任选地测定微生物和/或多核苷酸。

10. 发明申请

WO2012078426A3 MICROORGANISM CONCENTRATION PROCESS AND DEVICE 审中-公开
标题翻译：微生物浓缩过程和装置
公开(公告)号：WO2012078426A3
公开(公告)日：2012-10-04
申请号：PCT/US2011062618
申请日：2011-11-30
申请人： 3M INNOVATIVE PROPERTIES CO , KSHIRSAGAR MANJIRI T , RABINS ANDREW W
发明人： KSHIRSAGAR MANJIRI T , RABINS ANDREW W
IPC分类号： C12Q1/02 , C12M1/16 , C12M1/34 , C12Q1/06
CPC分类号： G01N1/405 , C12M47/02 , C12Q1/04 , C12Q1/24 , G01N1/40 , G01N33/84
摘要： A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising (1) a porous fibrous nonwoven matrix and (2) a plurality of particles of at least one concentration agent that comprises a metal silicate, the particles being enmeshed in the porous fibrous nonwoven matrix; (b) providing a sample comprising at least one target cellular analyte; (c) contacting the concentration device with the sample such that at least a portion of the at least one target cellular analyte is bound to or captured by the concentration device; and (d) detecting the presence of at least one bound target cellular analyte.
摘要翻译：（a）提供包含（1）多孔纤维非织造基质和（2）至少一种包含金属硅酸盐的浓集剂的多个颗粒的浓缩装置，所述颗粒嵌入多孔纤维非织造基质中; （b）提供包含至少一种靶细胞分析物的样品; （c）使所述浓缩装置与所述样品接触，使得所述至少一种目标细胞分析物的至少一部分结合到所述浓缩装置或由所述浓缩装置捕获; 和（d）检测至少一种结合靶细胞分析物的存在。

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