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1. 发明申请

WO1995007363A1 OPTICAL DETECTION OF POSITION OF OLIGONUCLEOTIDES ON LARGE DNA MOLECULES 审中-公开
标题翻译：对大分子DNA分子的寡核苷酸位置的光学检测
公开(公告)号：WO1995007363A1
公开(公告)日：1995-03-16
申请号：PCT/US1994009764
申请日：1994-09-08
申请人： GENEVUE, INC. , KONRAD, Michael, W.
发明人： GENEVUE, INC.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6813 , C12Q1/68 , C12Q1/6834
摘要： A method for analyzing a sample oligonucleotide sequence is described. The method comprises contacting the sample oligonucleotide sequence with an anchor sequence which comprises an immobilized oligonucleotide sequence which hybridizes with the sample. The sample is also contacted with a probe comprising an oligonucleotide sequence which hybridizes to a target oligonucleotide sequence to be detected in a suitable buffer to form a complex. The complex is subjected to a field which moves unbound oligonucleotide sequences away from the anchor sequence in the direction of the field, and preferably, extends the sample sequence. Whether the probe is bound to the sample oligonucleotide sequence, and preferably, the position of the probe, is determined to determine whether the target oligonucleotide sequence is present in the sample. The method can be used for mapping, for identity typing, and to determine whether a test oligonucleotide sequence is present in the sample. A device for performing the method and reagents are also described.
摘要翻译：描述了用于分析样品寡核苷酸序列的方法。该方法包括使样品寡核苷酸序列与包含与样品杂交的固定化寡核苷酸序列的锚定序列接触。样品也与包含寡核苷酸序列的探针接触，所述寡核苷酸序列与待合适缓冲液中待检测的靶寡核苷酸序列杂交以形成复合物。该复合物经受一个场，其将未结合的寡核苷酸序列沿场的方向移离锚定序列，优选地扩展样品序列。探针是否与样品寡核苷酸序列结合，优选探针的位置，以确定样品中是否存在靶寡核苷酸序列。该方法可用于映射，用于身份分类，并确定样品中是否存在测试寡核苷酸序列。还描述了用于执行该方法和试剂的装置。

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