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    • 2. 发明申请
    • APTAMER COATED MEASUREMENT AND REFERENCE ELECTRODES AND METHODS USING SAME FOR BIOMARKER DETECTION
    • APTAMER涂层测量和参考电极及使用该方法进行生物标记检测的方法
    • WO2012131403A4
    • 2012-11-29
    • PCT/GB2012050745
    • 2012-04-02
    • SAPIENT SENSORS LTDCASH STEPHEN LEEROBSON KEITHKINLOCH IAN ANTHONYSTOCKLEY PETER GEORGE
    • CASH STEPHEN LEEROBSON KEITHKINLOCH IAN ANTHONYSTOCKLEY PETER GEORGE
    • G01N33/53
    • G01N33/5438
    • A device for identifying the presence of a specific target molecule or biomarker by the detection of a change in an electrical property includes a measurement sensor 8 comprising a semiconducting sensor structure 12 capable of conjugating with the biomarker, thus giving rise to the said change in electrical property, and an electrode system 3, 4 for conducting a signal from the device. According to the invention there is a further such sensor 9, of substantially identical form but having its sensor structure 14 already conjugated with the biomarker, or otherwise capped, e.g. using a further oligonucleotide strand, so as to act as an internal reference. When a biological sample, e.g. saliva, is applied to the electrodes, the reference enables the discounting of all environmental effects other than the biomarker. The invention provides a simple, cheap and accurate text for one or more biomarkers that can be used in the field without complex equipment.
    • 用于通过检测电特性的变化来识别特定靶分子或生物标志物的存在的装置包括测量传感器8,其包括能够与生物标志物共轭的半导体传感器结构12,从而导致所述电气变化 以及用于从该装置传导信号的电极系统3,4。 根据本发明,还有一种这样的传感器9,其形状基本相同,但其传感器结构14已经与生物标志物缀合, 使用另外的寡核苷酸链,以作为内部参照。 当生物样品,例如 唾液被施用于电极,该参考物可以折射生物标志物以外的所有环境影响。 本发明为一种或多种生物标志物提供了一种简单,便宜且准确的文本,可用于现场,无需复杂的设备。
    • 6. 发明申请
    • APTAMER COATED MEASUREMENT AND REFERENCE ELECTRODES AND METHODS USING SAME FOR BIOMARKER DETECTION
    • APTAMER涂层测量和参考电极和使用它们进行生物标志物检测的方法
    • WO2012131403A1
    • 2012-10-04
    • PCT/GB2012/050745
    • 2012-04-02
    • SAPIENT SENSORS LIMITEDCASH, Stephen LeeROBSON, KeithKINLOCH, Ian AnthonySTOCKLEY, Peter George
    • CASH, Stephen LeeROBSON, KeithKINLOCH, Ian AnthonySTOCKLEY, Peter George
    • G01N33/53
    • G01N33/5438
    • A device for identifying the presence of a specific target molecule or biomarker by the detection of a change in an electrical property includes a measurement sensor 8 comprising a semiconducting sensor structure 12 capable of conjugating with the biomarker, thus giving rise to the said change in electrical property, and an electrode system 3, 4 for conducting a signal from the device. According to the invention there is a further such sensor 9, of substantially identical form but having its sensor structure 14 already conjugated with the biomarker, or otherwise capped, e.g. using a further oligonucleotide strand, so as to act as an internal reference. When a biological sample, e.g. saliva, is applied to the electrodes, the reference enables the discounting of all environmental effects other than the biomarker. The invention provides a simple, cheap and accurate text for one or more biomarkers that can be used in the field without complex equipment.
    • 用于通过检测电特性的变化来识别特定靶分子或生物标志物的存在的装置包括测量传感器8,其包括能够与生物标志物缀合的半导体传感器结构12,因此 引起所述电特性的变化,以及用于传导来自装置的信号的电极系统3,4。 根据本发明,还有另一个这样的传感器9,其具有基本上相同的形式,但是其传感器结构14已经与生物标志物缀合,或者以其他方式例如被封盖。 使用另外的寡核苷酸链,以充当内部参考。 当生物样本例如 唾液应用于电极,该参考使得能够折扣除生物标志物以外的所有环境影响。 本发明为一种或多种生物标志物提供简单,便宜和准确的文本,可以在没有复杂设备的情况下用于现场。