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1. 发明申请

WO2008146992A1 PROCESS FOR THE HIGH-PURITY ISOLATION OF MESENCHYMAL STEM CELLS DERIVED FROM PLACENTAL CHORIONIC PLATE MEMBRANE 审中-公开
标题翻译：高纯度分离从普通氯胺酮薄膜衍生的中胚层干细胞的方法
公开(公告)号：WO2008146992A1
公开(公告)日：2008-12-04
申请号：PCT/KR2007/005235
申请日：2007-10-24
申请人： CHABIOTECH CO., LTD. , COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION , KIM, Gi-Jin , SHIN, Kyung-Sun , NA, Kyu-Hwan , CHUNG, Hyung-Min
发明人： KIM, Gi-Jin , SHIN, Kyung-Sun , NA, Kyu-Hwan , CHUNG, Hyung-Min
IPC分类号： C12N5/08 , C12N5/06
CPC分类号： C12N5/0605 , C12N5/0668 , C12N2501/119 , C12N2501/91
摘要： The present invention provides a method for isolating mesenchymal stem cells derived from a placental chorionic plate membrane, the method including: (a) harvesting a chorionic plate membrane from a detached placenta; (b) harvesting cells present in the chorionic plate membrane obtained in step (a) by scraping; (c) adding a solution containing trypsin and ethylenediaminetetraacetate to the cells obtained in step (b) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; and (d) centrifuging the reaction solution obtained in step (c) and culturing the obtained cells in a medium containing a fetal bovine serum and an antibiotic.
摘要翻译：本发明提供了一种分离源自胎盘绒膜膜的间充质干细胞的方法，所述方法包括：（a）从分离的胎盘中收获绒毛膜板; （b）通过刮擦收集在步骤（a）中获得的绒毛膜板中存在的细胞; （c）向步骤（b）中获得的细胞中加入含有胰蛋白酶和乙二胺四乙酸的溶液进行酶促反应并加入胎牛血清以终止酶促反应; 和（d）将步骤（c）中获得的反应溶液离心，并将所获得的细胞培养在含有胎牛血清和抗生素的培养基中。

2. 发明申请

WO2003065397A1 CONTACT SWITCH DEVICE USING MAGNETS 审中-公开
标题翻译：触点切换装置使用磁铁
公开(公告)号：WO2003065397A1
公开(公告)日：2003-08-07
申请号：PCT/KR2003/000209
申请日：2003-01-29
申请人： KIM, Gi Jin
发明人： KIM, Gi Jin
IPC分类号： H01H36/00
CPC分类号： H01H35/36 , H01H3/40 , H01H36/0073
摘要： Disclosed is a contact switch device including a first arc-shaped gear (13) connected to a Bourdon tube, a second arc-shaped gear (18) rotating in the same direction as a pinion gear (14) engaged with the gear (13) while rotating reversely in accordance with a reaction thereof generated against the rotation of the pinion gear (14) when it comes into contact with a stopper (16 or 17), a pivoting magnet (22) pivoting in accordance with rotation of an assistant pinion gear engaged with the gear (18), a moving magnet (23) supported by a plate spring (25), and adapted to switch off contacts against the resilience of the plate spring when it is aligned with the pivoting magnet, and fixed magnet (24) for maintianing contacts in a switched-on state when it is aligned with the pivoting magnet. The plate spring switches on the contacts when the pivoting magnet is misaligned from the moving magnet.
摘要翻译：公开了一种接触开关装置，包括连接到布尔登管的第一弧形齿轮（13），与与齿轮（13）接合的小齿轮（14）相同方向旋转的第二弧形齿轮（18）同时当其与止动件（16或17）接触时，根据小齿轮（14）的旋转产生的反作用而反向旋转;枢转磁体（22），其根据辅助小齿轮的旋转而枢转与所述齿轮（18）接合，由板弹簧（25）支撑的移动磁体（23），并且适于在与所述枢转磁体对准的情况下将所述触点抵抗所述板簧的弹性而断开;以及固定磁体），当与枢转磁体对准时，主导触点处于接通状态。当枢转磁体与移动的磁体不对准时，板簧在触点上打开。

3. 发明申请

WO2008146991A1 PROCESS FOR THE ISOLATION OF PLACENTA-DERIVED TROPHOBLAST STEM CELLS 审中-公开
标题翻译：用于分离衍生的TROPHOBLAST干细胞的方法
公开(公告)号：WO2008146991A1
公开(公告)日：2008-12-04
申请号：PCT/KR2007/005234
申请日：2007-10-24
申请人： CHABIOTECH CO., LTD. , COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION , KIM, Gi-Jin , SHIN, Kyung-Sun , NA, Kyu-Hwan
发明人： KIM, Gi-Jin , SHIN, Kyung-Sun , NA, Kyu-Hwan
IPC分类号： C12N5/08 , C12N5/06
CPC分类号： C12N5/0605 , C12N5/0606 , C12N2500/44 , C12N2500/46 , C12N2501/119 , C12N2501/70 , C12N2501/91
摘要： The present invention provides a method for isolating trophoblast stem cells, the method including: (a) harvesting placental villi from a detached normal placenta; (b) adding an enzyme solution containing trypsin, DMase I, and dispase to the placental villi of step (a) to perform an enzymatic reaction and adding a fetal bovine serum thereto to terminate the enzymatic reaction; (c) centrifuging the reaction solution of step (b) and separating cytotrophoblasts from the recovered cells by a density-gradient separation method; and (d) culturing the cytotrophoblasts of step (c) in a medium containing a fetal bovine serum and an antibiotic.
摘要翻译：本发明提供了一种分离滋养层干细胞的方法，所述方法包括：（a）从分离的正常胎盘中收获胎盘绒毛; （b）向步骤（a）的胎盘绒毛中加入含有胰蛋白酶，DMase I和分泌酶的酶溶液进行酶促反应，并加入胎牛血清以终止酶促反应; （c）离心步骤（b）的反应溶液，并通过密度梯度分离方法从回收的细胞中分离细胞滋养层细胞; 和（d）在含有胎牛血清和抗生素的培养基中培养步骤（c）的滋养细胞。

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