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1. 发明申请

WO1998008975A1 METHODS FOR TARGETING, ENRICHING, DETECTING AND/OR ISOLATING TARGET NUCLEIC ACID SEQUENCE USING RECA-LIKE RECOMBINASE 审中-公开
标题翻译：用RECA-like重组体靶向，增强，检测和/或分离靶向核酸序列的方法
公开(公告)号：WO1998008975A1
公开(公告)日：1998-03-05
申请号：PCT/JP1997003019
申请日：1997-08-29
申请人： DAIKIN INDUSTRIES, LTD. , KIGAWA, Koji , YAMANAKA, Mikayo , KUSUMI, Kayo , MUKAI, Eli , OBATA, Kazuaki
发明人： DAIKIN INDUSTRIES, LTD.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6839 , C12N15/1093 , C12Q1/6841 , C12Q2521/507
摘要： A method for targeting, enriching, detecting and isolating a double-stranded nucleic acid target sequence by adding heterologous probes to the reaction system along with the reduced amount of homologous probes in the process of homologous pairing and/or strand exchange between the homologous probe and the target sequence in the presence of a RecA-like recombinase.
摘要翻译：一种用于靶向，富集，检测和分离双链核酸靶序列的方法，其通过将异源探针与同源探针和/或同源探针和/或同源探针之间的同源配对和/或链交换过程中减少量的同源探针一起加入到反应系统中在RecA样重组酶存在下的靶序列。

2. 发明申请

WO1995018236A1 IN-SITU HYBRIDIZATION METHOD USING RecA PROTEIN AND RecA PROTEIN HAVING MARKER OR LIGAND FOR USE IN SAID METHOD 审中-公开
标题翻译：使用具有标记或配体的RecA蛋白和RecA蛋白的现场混合方法用于描述方法
公开(公告)号：WO1995018236A1
公开(公告)日：1995-07-06
申请号：PCT/JP1994002276
申请日：1994-12-27
申请人： DAIKIN INDUSTRIES, LTD. , KIGAWA, Koji , YAMANAKA, Mikayo , KIHARA, Kayo , MUKAI, Eli , OBATA, Kazuaki
发明人： DAIKIN INDUSTRIES, LTD.
IPC分类号： C12Q01/68
CPC分类号： C12Q1/6804 , C12Q1/6813 , C12Q1/6816 , C12Q1/6841 , Y10S435/81
摘要： A method for detecting the presence of a target double-stranded nucleic acid sequence in an immobilized cell or cell structure, which method involves the step of immobilizing a cell or a cell structure in such a manner as to permit the invasion by a nucleic acid probe and the step of preparing a probe-RecA composite comprising a single-stranded probe and a RecA protein bonded to each other stably. The presence of a target double-stranded nucleic acid sequence is detected by binding the probe-RecA composite to a target double-stranded nucleic acid sequence through the reaction therebetween under the condition where the target nucleic acid sequence does not undergo denaturation and detecting the RecA protein contained in the composite. The invention method provides a diagnostically important method whereby the position of a specified gene or a regulatory region thereof in a chromosome or the presence of a virus-origin nucleic acid sequence can be determined or visualized readily at a high sensitivity.
摘要翻译：一种用于检测固定化细胞或细胞结构中靶双链核酸序列的存在的方法，该方法包括以允许核酸探针侵入的方式固定细胞或细胞结构的步骤以及制备包含稳定地彼此结合的单链探针和RecA蛋白质的探针-ReAA复合物的步骤。通过在靶核酸序列未经历变性并检测RecA的条件下，通过其间的反应将探针-RacA复合物与靶双链核酸序列结合来检测靶双链核酸序列的存在蛋白质包含在复合材料中。本发明方法提供诊断上重要的方法，其中可以以高灵敏度容易地确定或可视化在染色体中特定基因或其调节区的位置或病毒来源的核酸序列的存在。

3. 发明申请

WO2008041998A1 CHAIN END FUNCTIONALIZED FLUOROPOLYMERS HAVING GOOD ELECTRICAL PROPERTIES AND GOOD CHEMICAL REACTIVITY 审中-公开
标题翻译：具有良好电性能和良好的化学反应性的链端官能化荧光体
公开(公告)号：WO2008041998A1
公开(公告)日：2008-04-10
申请号：PCT/US2006/042473
申请日：2006-10-31
申请人： THE PENN STATE RESEARCH FOUNDATION , CHUNG, Tze-Chiang , ZHANG, Zhicheng , CHEN, Shangtao , KUBO, Katsuyoshi , KIGAWA, Koji
发明人： CHUNG, Tze-Chiang , ZHANG, Zhicheng , CHEN, Shangtao , KUBO, Katsuyoshi , KIGAWA, Koji
IPC分类号： C08F214/22 , C08F8/00 , C08L27/16
CPC分类号： C08F214/22 , C08F8/00 , C08F8/04 , C08F8/14 , C08F214/225 , C08F2800/10 , C08F2800/20 , C08F2810/20 , C08F2810/40 , C08J3/28 , C08J2327/16 , C08L27/16 , Y10T428/3154 , C08F14/18 , C08L2666/04
摘要： Chain end functionalized fluoropolymers are disclosed exhibiting good chemical reactivity high breakdown electric field (E > 100 MV/m) and high dielectric constants (i.e., ε > 10). The polymers can be prepared by functional initiators and from a variety of fluoromonomers including vinylidene difluoride together with one or more additional monomers. The polymers can be combined with other dielectric materials to form composites having uniform composition and morphology.
摘要翻译：公开了具有良好的化学反应性高击穿电场（E> 100MV / m）和高介电常数（即e> 10）的链端官能化含氟聚合物。聚合物可以通过官能引发剂和各种含氟单体（包括亚乙烯基二氟化物）与一种或多种另外的单体一起制备。聚合物可与其它电介质材料组合以形成具有均匀组成和形态的复合材料。

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