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    • 8. 发明申请
    • A METHOD THAT COMPARES GENOMIC SEQUENCES
    • 比较基因组序列的方法
    • WO0162967A2
    • 2001-08-30
    • PCT/IL0100151
    • 2001-02-19
    • GENENA LTDAGRICULTURAL RES ORGVIDER BEN ZIONKATZIR NURIT
    • VIDER BEN-ZIONKATZIR NURIT
    • C12Q1/68
    • C12Q1/6809C12Q1/6855C12Q2525/191C12Q2525/186C12Q2525/179
    • Described herein is a PCR method of DNA fingerprinting using primers that match conserved regions of a gene family. In this manner the resulting PCR products represents genomic DNA fragments that are parts of genes. The DNA fingerprints can be those of any source, plant, animal and human. The method uses a plurality of sense primers that match a first conserved region in the gene family, and a plurality of anti-sense primers that match another conserved region in the gene family. By several modifications enabling increased spectrum of variability one set of conserved primers can be replaced by primers that match the sequences of microsatellite repeat, or cis-acting regulatory region or any other random primer. The DNA fingerprinting system can be linked to these genes expression patterns in various organs and in various biological situations.
    • 本文描述了使用与基因家族的保守区域相匹配的引物的DNA指纹图谱的PCR方法。 以这种方式,得到的PCR产物表示作为基因部分的基因组DNA片段。 DNA指纹可以是任何来源,植物,动物和人类的指纹图谱。 该方法使用与基因家族中的第一保守区相匹配的多个有义引物和与基因家族中的另一个保守区相匹配的多个反义引物。 通过若干修改使得增加的变异谱可以将一组保守引物替换为与微卫星重复序列或顺式作用调节区或任何其它随机引物的序列匹配的引物。 DNA指纹系统可以与各种器官和各种生物学情况下的这些基因表达模式相关联。