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2. 发明申请

WO2004042403A2 METHODS, DEVICE AND INSTRUMENT FOR DETECTION OF ANALYTES 审中-公开
标题翻译：用于检测分析仪的方法，装置和仪器
公开(公告)号：WO2004042403A2
公开(公告)日：2004-05-21
申请号：PCT/EP2003/012234
申请日：2003-11-03
申请人： LUDWIG-MAXIMILIAN-UNI VERSITÄT MÜNCHEN , ROCHE DIAGNOSTICS GMBH , FELDMANN, Jochen , FRANZL, Thomas , KLAR, Thomas , RASCHKE, Gunnar , SÖNNICHSEN, Carsten , KOWARIK, Stefan , NICHTL, Alfons , KÜRZINGER, Konrad
发明人： FELDMANN, Jochen , FRANZL, Thomas , KLAR, Thomas , RASCHKE, Gunnar , SÖNNICHSEN, Carsten , KOWARIK, Stefan , NICHTL, Alfons , KÜRZINGER, Konrad
IPC分类号： G01N33/58
CPC分类号： G01N33/54346 , G01N15/1468 , G01N21/554
摘要： The present invention relates to a method, a device and an instrument for the detection of an analyte (4) in a sample(6). Such method, device and instrument is used in clinical diagnostics, pharmacology, e.g. drug screening, environmental analysis as well as in other fields of analysis like chemistry or biochemistry. According to the present invention, the method for detection of an analyte (4) in a sample comprises the following steps of a) providing at least one particle structure (1) detectable by light scattering and coated with binding entities (2) for said analyte (4); b) contacting the at least one particle structure (1) with the sample (6), c) illuminating the at least one particle structure (1) and measuring the scattered light emitted by individual particle structures (1) at least twice and d) determining a change in the spectral signature of the scattered light as a measure of the presence and/or quantity of the analyte (4) in the sample (6). In order to detect more than one analyte (4) simultaneously particle structures (1) of different classes of particle structures maybe coated with different binding entities. Further, instead of coatig the particle structure (1) with binding entities specific for the analyte (4), the particle structure (1) may be coated with substances (21) competing with said analyte (4) for binding to specific binding entities (2). Any competitive and non-competitive assay format can be put into practice.
摘要翻译：本发明涉及用于检测样品（6）中的分析物（4）的方法，装置和仪器。这种方法，装置和仪器用于临床诊断，药理学，例如。药物筛选，环境分析以及化学或生物化学等其他分析领域。根据本发明，用于检测样品中的分析物（4）的方法包括以下步骤：a）提供至少一个可通过光散射检测的颗粒结构（1），并涂覆有用于所述分析物的结合实体（2）（4）; b）使所述至少一个颗粒结构（1）与所述样品（6）接触，c）照射所述至少一个颗粒结构（1）并且测量由各个颗粒结构（1）发射的散射光至少两次，以及d）确定散射光的光谱特征的变化，作为样品（6）中分析物（4）的存在和/或量的量度。为了检测多于一种分析物（4），不同类型的颗粒结构的颗粒结构（1）可以同时涂覆不同的结合实体。此外，代替用特定于分析物（4）的结合实体对颗粒结构（1）进行涂层，颗粒结构（1）可以涂覆与所述分析物（4）竞争的物质（21）以结合特异性结合实体（ 2）。任何竞争性和非竞争性的测定格式都可以付诸实践。

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