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1. 发明申请

WO2007148926A9 TFLA GENE WHICH CAN DEGRADE TOXOFLAVIN AND ITS CHEMICAL DERIVATIVES AND TRANSGENIC ORGANISMS EXPRESSING TFLA GENE 审中-公开
标题翻译：可降解托吡芬的TFLA基因及其表达TFLA基因的化学衍生物和转基因
公开(公告)号：WO2007148926A9
公开(公告)日：2009-01-29
申请号：PCT/KR2007003010
申请日：2007-06-21
申请人： SNU R&DB FOUNDATION , HWANG IN GYU , MOON JAE SUN , JWA NAM SOO
发明人： HWANG IN GYU , MOON JAE SUN , JWA NAM SOO
IPC分类号： C12N1/20 , C12N9/00 , C12N15/00
CPC分类号： C12N9/0071 , C12N15/821
摘要： The present invention relates to a microorganism which can degrade toxoflavin and its derivatives, a protein which can degrade toxoflavin and its derivatives, a use of said protein as a selection marker for plant transformation, a gene which encodes said protein, a recombinant expression vector comprising said gene, a transgenic organism which is transformed with said vector, an expression cassette of a selection marker comprising tflA gene for plant transformation, a recombinant vector comprising said expression cassette, a plant which is transformed with said vector, a method of selecting transgenic plants using tflA gene, and a method of preparing transgenic plants using tflA gene. According to the present invention, transgenic plants which express tflA are given with the resistant character to toxoflavin. Particularly for rice cultivation, more grains can be harvested and quality of grains can be improved, thanks to a resistance to bacterial grain rot. In addition, instead of using expensive antibiotics, transgenic plants can be easily selected by using rather cheap toxoflavin.
摘要翻译：本发明涉及可降解毒黄素及其衍生物的微生物，可降解毒黄色素及其衍生物的蛋白质，使用所述蛋白质作为植物转化的选择标记物，编码所述蛋白质的基因，重组表达载体，其包含所述基因是用所述载体转化的转基因生物，包含用于植物转化的tflA基因的选择标记的表达盒，包含所述表达盒的重组载体，用所述载体转化的植物，选择转基因植物的方法使用tflA基因，以及使用tflA基因制备转基因植物的方法。根据本发明，表达tflA的转基因植物具有对毒死黄素的抗性。特别是对于水稻种植，由于抗细菌颗粒腐烂，可以收获更多的谷物并提高谷粒质量。此外，代替使用昂贵的抗生素，可以通过使用相当便宜的毒黄素来容易地选择转基因植物。

2. 发明申请

WO2007148926A1 TFLA GENE WHICH CAN DEGRADE TOXOFLAVIN AND ITS CHEMICAL DERIVATIVES AND TRANSGENIC ORGANISMS EXPRESSING TFLA GENE 审中-公开
标题翻译：可降解托吡芬的TFLA基因及其表达TFLA基因的化学衍生物和转基因
公开(公告)号：WO2007148926A1
公开(公告)日：2007-12-27
申请号：PCT/KR2007/003010
申请日：2007-06-21
申请人： SEOUL NATIONAL UNIVERSITY INDUSTRY FOUNDATION , HWANG, In Gyu , MOON, Jae Sun , JWA, Nam Soo
发明人： HWANG, In Gyu , MOON, Jae Sun , JWA, Nam Soo
IPC分类号： C12N1/20 , C12N9/00 , C12N15/00
CPC分类号： C12N9/0071 , C12N15/821
摘要： The present invention relates to a microorganism which can degrade toxoflavin and its derivatives, a protein which can degrade toxoflavin and its derivatives, a use of said protein as a selection marker for plant transformation, a gene which encodes said protein, a recombinant expression vector comprising said gene, a transgenic organism which is transformed with said vector, an expression cassette of a selection marker comprising tflA gene for plant transformation, a recombinant vector comprising said expression cassette, a plant which is transformed with said vector, a method of selecting transgenic plants using tflA gene, and a method of preparing transgenic plants using tflA gene. According to the present invention, transgenic plants which express tflA are given with the resistant character to toxoflavin. Particularly for rice cultivation, more grains can be harvested and quality of grains can be improved, thanks to a resistance to bacterial grain rot. In addition, instead of using expensive antibiotics, transgenic plants can be easily selected by using rather cheap toxoflavin.
摘要翻译：本发明涉及可降解毒黄素及其衍生物的微生物，可降解毒黄色素及其衍生物的蛋白质，使用所述蛋白质作为植物转化的选择标记物，编码所述蛋白质的基因，重组表达载体，其包含所述基因是用所述载体转化的转基因生物，包含用于植物转化的tflA基因的选择标记的表达盒，包含所述表达盒的重组载体，用所述载体转化的植物，选择转基因植物的方法使用tflA基因，以及使用tflA基因制备转基因植物的方法。根据本发明，表达tflA的转基因植物具有对毒死黄素的抗性。特别是对于水稻种植，由于抗细菌颗粒腐烂，可以收获更多的谷物并提高谷粒质量。此外，代替使用昂贵的抗生素，可以通过使用相当便宜的毒黄素来容易地选择转基因植物。

3. 发明申请

WO2009016232A8 PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有增强的相关旅行者的植物及其制造方法
公开(公告)号：WO2009016232A8
公开(公告)日：2010-01-21
申请号：PCT/EP2008060060
申请日：2008-07-31
申请人： BASF PLANT SCIENCE GMBH , CROP FUNCTIONAL GENOMICS CT , KIM WOO TAEK , CHOI SEOK KEUN , HWANG IN GYU , MOON JAE SUN , JWA NAM-SOO , CHOI OKHEE , CHOI YANG DO , PARK YOUN-II , CHUNG SUCK WON
发明人： KIM WOO TAEK , CHOI SEOK KEUN , HWANG IN GYU , MOON JAE SUN , JWA NAM-SOO , CHOI OKHEE , CHOI YANG DO , PARK YOUN-II , CHUNG SUCK WON
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8261 , C12N9/001 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a yield increasing polypeptide selected from the group consisting of: phosphoenolpyruvate carboxylase (PEPC), Class III U-Box protein and PQQC protein. The present invention also concerns plants having modulated expression of a nucleic acid encoding yield increasing polypeptide selected from the group consisting of: phosphoenolpyruvate carboxylase (PEPC), Class III U-Box protein and PQQC protein, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及一种通过调节植物中编码增产多肽的核酸表达来增强植物中产量相关性状的方法，所述产量增加多肽选自：磷酸烯醇丙酮酸羧化酶（PEPC），III类U盒蛋白和PQQC蛋白。本发明还涉及具有编码增产多肽的核酸调节表达的植物，其选自：磷酸烯醇丙酮酸羧化酶（PEPC），III类U盒蛋白和PQQC蛋白，该植物具有增强的产量相关性状相对于相应的野生型植物或其他对照植物。本发明还提供了可用于本发明方法的构建体。

4. 发明申请

WO2009054564A1 METHOD FOR THE SYNTHESIS OF NUCLEIC ACID BY USING FRAGMENTED NUCLEIC ACIDS AND N-PRIMER 审中-公开
标题翻译：通过使用裂解的核酸和N-PRIMER合成核酸的方法
公开(公告)号：WO2009054564A1
公开(公告)日：2009-04-30
申请号：PCT/KR2007/006070
申请日：2007-11-28
申请人： KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHNOLOGY , MOON, Jae Sun , KIM, Sung Uk , HWANG, In Gyu , JWA, Nam Soo , KWON, Suk Yoon
发明人： MOON, Jae Sun , KIM, Sung Uk , HWANG, In Gyu , JWA, Nam Soo , KWON, Suk Yoon
IPC分类号： C12N15/09 , C12N15/11 , C12Q1/68
CPC分类号： C12N15/1096 , C12N15/1093 , C12Q1/6844 , C12Q2523/301 , C12Q2521/107 , C12Q2525/179
摘要： The present invention relates to a method for the synthesis of nucleic acid based on RNA fragmentation and N-primer which is prepared by using a fragmented RNA as a template, a method for constructing a cDNA library, a cDNA library constructed by said method, and a kit comprising N-primer for the synthesis of nucleic acid. According to the present invention, N-primer which may have the same complementarity for all regions of mRNA and various kinds of nucleic acids including mRNAs that are randomly fragmented by an artificial method are used for the synthesis of cDNAs or strands of complementary nucleic acids for every region. Thus, an overlap can be avoided and all kinds of cDNAs originating from mRNA or base sequences of the nucleic aids that are complementary to mRNA can be obtained.
摘要翻译：本发明涉及一种基于RNA片段化的核酸的合成方法和通过使用片段化RNA作为模板制备的N-引物，构建cDNA文库的方法，通过所述方法构建的cDNA文库，以及包含用于合成核酸的N-引物的试剂盒。根据本发明，可以对mRNA的所有区域具有相同的互补性的N-引物和包括通过人造方法随机碎裂的mRNA的各种核酸用于合成互补核酸的cDNA或链，用于每个地区。因此，可以避免重叠，可以获得源自与mRNA互补的核酸辅助子的mRNA或碱基序列的各种cDNA。

5. 发明申请

WO2009016232A3 PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME 审中-公开
公开(公告)号：WO2009016232A3
公开(公告)日：2009-02-05
申请号：PCT/EP2008/060060
申请日：2008-07-31
申请人： BASF PLANT SCIENCE GMBH , CROP FUNCTIONAL GENOMICS CENTER , KIM, Woo, Taek , CHOI, Seok, Keun , HWANG, In, Gyu , MOON, Jae Sun , JWA, Nam-Soo , CHOI, Okhee , CHOI, Yang Do , PARK, Youn-II , CHUNG, Suck Won
发明人： KIM, Woo, Taek , CHOI, Seok, Keun , HWANG, In, Gyu , MOON, Jae Sun , JWA, Nam-Soo , CHOI, Okhee , CHOI, Yang Do , PARK, Youn-II , CHUNG, Suck Won
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a yield increasing polypeptide selected from the group consisting of: phosphoenolpyruvate carboxylase (PEPC), Class III U-Box protein and PQQC protein. The present invention also concerns plants having modulated expression of a nucleic acid encoding yield increasing polypeptide selected from the group consisting of: phosphoenolpyruvate carboxylase (PEPC), Class III U-Box protein and PQQC protein, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.

6. 发明申请

WO2009016232A2 PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME 审中-公开
标题翻译：具有增强的产量相关性状的植物及其制备方法
公开(公告)号：WO2009016232A2
公开(公告)日：2009-02-05
申请号：PCT/EP2008060060
申请日：2008-07-31
申请人： BASF PLANT SCIENCE GMBH , CROP FUNCTIONAL GENOMICS CT , KIM WOO TAEK , CHOI SEOK KEUN , HWANG IN GYU , MOON JAE SUN , JWA NAM-SOO , CHOI OKHEE , CHOI YANG DO , PARK YOUN-II , CHUNG SUCK WON
发明人： KIM WOO TAEK , CHOI SEOK KEUN , HWANG IN GYU , MOON JAE SUN , JWA NAM-SOO , CHOI OKHEE , CHOI YANG DO , PARK YOUN-II , CHUNG SUCK WON
IPC分类号： C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8261 , C12N9/001 , C12N15/8273 , Y02A40/146
摘要： The present invention relates generally to the field of molecular biology and concerns a method for enhancing yield-related traits in plants by modulating expression in a plant of a nucleic acid encoding a yield increasing polypeptide selected from the group consisting of: phosphoenolpyruvate carboxylase (PEPC), Class III U-Box protein and PQQC protein. The present invention also concerns plants having modulated expression of a nucleic acid encoding yield increasing polypeptide selected from the group consisting of: phosphoenolpyruvate carboxylase (PEPC), Class III U-Box protein and PQQC protein, which plants have enhanced yield-related traits relative to corresponding wild type plants or other control plants. The invention also provides constructs useful in the methods of the invention.
摘要翻译：本发明一般涉及分子生物学领域，涉及一种通过调节植物中编码产量增加多肽的核酸的表达来增强植物中产量相关性状的方法，所述产量增加多肽选自：磷酸烯醇式丙酮酸羧化酶（PEPC），III类U-Box蛋白和PQQC蛋白。本发明还涉及具有调节的表达编码产量增加多肽的核酸的植物，所述产量增加多肽选自磷酸烯醇式丙酮酸羧化酶（PEPC），III类U-Box蛋白和PQQC蛋白，所述植物具有增强的产量相关性状相应的野生型植物或其他对照植物。本发明还提供了可用于本发明方法的构建体。

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