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1. 发明申请

WO2004087887A2 INTRACELLULAR COMPLEXES AS BIOMARKERS 审中-公开
标题翻译：细胞内复合物作为生物标志物
公开(公告)号：WO2004087887A2
公开(公告)日：2004-10-14
申请号：PCT/US2004009805
申请日：2004-03-30
申请人： ACLARA BIOSCIENCES INC , SINGH SHARAT , BADAL M YOUSSOUF , JIN XUENGUANG , SALIMI-MOOSAVI HOSSEIN
发明人： SINGH SHARAT , BADAL M YOUSSOUF , JIN XUENGUANG , SALIMI-MOOSAVI HOSSEIN
IPC分类号： A61B20060101 , C07K16/28 , C12N20060101 , C12Q3/00 , G01N33/542 , G01N33/543 , G01N33/574 , C12N
CPC分类号： G01N33/57492 , C07K16/2863 , G01N33/542 , G01N2333/485 , G01N2800/52
摘要： The invention provides a method for determining a disease status of a patient by measuring expression levels of selected intracellular complexes. In one aspect of the invention, the activation status of apoptotic pathways in a patient sample is determined by measuring relative amounts of protein-protein complexes that are characteristic of the apoptotic pathways. In particular, the invention provides a method of determining the activation status of the mitochondrial apoptotic pathway by simultaneously measuring relative amounts of complexes between 14-3-3 proteins and BAD proteins on the one hand and complexes of Bcl-2 proteins and BAD proteins on the other hand. Preferably, methods of the invention are implemented by using sets of binding compounds having releasable molecular tags that are specific for multiple components of one or more complexes formed within apoptotic pathways. After binding molecular tags are released and separated from the assay mixture for analysis.
摘要翻译：本发明提供了一种通过测量所选细胞内复合物的表达水平来确定患者的疾病状态的方法。在本发明的一个方面，通过测量凋亡通路特征的蛋白质 - 蛋白质复合物的相对量来确定患者样品中凋亡途径的活化状态。特别地，本发明提供了一种通过同时测量14-3-3蛋白和BAD蛋白之间的复合物的相对量以及Bcl-2蛋白和BAD蛋白的复合物的相对量来确定线粒体凋亡途径的激活状态的方法另一方面。优选地，本发明的方法通过使用具有可释放分子标签的结合化合物组来实现，所述可释放分子标签对在凋亡途径内形成的一种或多种复合物的多种组分是特异性的。结合分子标签后，从测定混合物中分离出分子标签进行分析。

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