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1. 发明申请

WO2005024053A1 NUCLEIC ACID DETECTION ASSAY 审中-公开
标题翻译：核酸检测测定
公开(公告)号：WO2005024053A1
公开(公告)日：2005-03-17
申请号：PCT/AU2004/001196
申请日：2004-09-03
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , MELKI, John, Robert , MIKLOS, George, L., Gabor
发明人： MILLAR, Douglas, Spencer , MELKI, John, Robert , MIKLOS, George, L., Gabor
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2533/107 , C12Q2525/307 , C12Q2523/125 , C12Q2521/325
摘要： A method for determining the methylation status of a potential methylation site in genomic nucleic acid comprising treating genomic nucleic acid with an agent which modifies cytosine bases but does not modify 5methyl-cytosine bases under conditions to form a. modified nucleic acid template containing a potential methylation site; providing a first clamp containing a first capture sequence complementary to a region flanking one side of the potential methylation site in the modified nucleic acid template, providing a second clamp containing a second capture sequence complementary to a region flanking the other side of the potential methylation site in the modified nucleic acid template; allowing the first clamp and the second clamp to hybridise to the modified nucleic acid template; ligating the hybridised first and second clamps to form a probe spanning the potential methylation site in the modified nucleic acid template; digesting the modified nucleic acid template to obtain the probe; and detecting the probe and determining the methylation status of the potential methylation site in the modified genomic nucleic acid.
摘要翻译：一种确定基因组核酸中潜在的甲基化位点的甲基化状态的方法，其包括用修饰胞嘧啶碱基但不改变5个甲基胞嘧啶碱基的试剂处理基因组核酸。含有潜在甲基化位点的修饰的核酸模板; 提供第一夹具，其含有与修饰的核酸模板中的潜在甲基化位点侧翼侧的区域互补的第一捕获序列，提供包含与潜在甲基化位点的另一侧侧翼区域互补的第二捕获序列的第二夹在修饰的核酸模板中; 允许第一夹具和第二夹钳与修饰的核酸模板杂交; 连接杂交的第一和第二夹具以形成跨越修饰的核酸模板中的潜在甲基化位点的探针; 消化修饰的核酸模板以获得探针; 并检测探针并测定修饰基因组核酸中潜在甲基化位点的甲基化状态。

2. 发明申请

WO2013033749A1 MOLECULAR DETECTION ASSAY 审中-公开
标题翻译：分子检测测定
公开(公告)号：WO2013033749A1
公开(公告)日：2013-03-14
申请号：PCT/AU2011/001156
申请日：2011-09-07
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLER, Douglas, Spencer
发明人： MILLER, Douglas, Spencer
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6886 , C12Q1/689 , C12Q1/6893 , C12Q1/707 , C12Q2600/158 , C12Q2523/125
摘要： The present invention relates to a molecular detection assay comprising treating a biological sample directly with a bisulphite agent under conditions that allow cell disruption and nucleic acid treatment; removing the bisulphite agent from the treated sample; and detecting a target nucleic acid in the treated sample.
摘要翻译：本发明涉及分子检测测定法，其包括在允许细胞破碎和核酸处理的条件下用亚硫酸氢盐剂直接处理生物样品; 从处理过的样品中除去亚硫酸氢盐; 并检测被处理样品中的靶核酸。

3. 发明申请

WO2009070843A1 BISULPHITE TREATMENT OF RNA 审中-公开
标题翻译： RNA的双歧杆菌处理
公开(公告)号：WO2009070843A1
公开(公告)日：2009-06-11
申请号：PCT/AU2008/001796
申请日：2008-12-04
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , MELKI, John, R.
发明人： MILLAR, Douglas, Spencer , MELKI, John, R.
IPC分类号： C07B41/06 , C01D5/00 , C07H21/02
CPC分类号： C07H21/02
摘要： The invention relates to a method for bisulphite treating RNA comprising reacting RNA with a bisulphite reagent at 50-90°C for 5-180 minutes so as to form treated RNA and recovering the treated RNA.
摘要翻译：本发明涉及亚硫酸氢盐处理RNA的方法，其包括在50-90℃下将RNA与亚硫酸氢盐试剂反应5-180分钟，以形成经处理的RNA并回收经处理的RNA。

4. 发明申请

WO2007030882A1 ASSAY FOR A HEALTH STATE 审中-公开
标题翻译：测定健康状况
公开(公告)号：WO2007030882A1
公开(公告)日：2007-03-22
申请号：PCT/AU2006/001349
申请日：2006-09-14
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , MELKI, John, R. , MIKLOS, George, L., Gabor
发明人： MILLAR, Douglas, Spencer , MELKI, John, R. , MIKLOS, George, L., Gabor
IPC分类号： C12Q1/68 , G01N33/574
CPC分类号： C12Q1/6883 , C12Q2600/154 , C12Q2600/16
摘要： The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state.
摘要翻译：本发明涉及使用组合检测病毒的存在并检测指示健康状态的基因组靶标或标志物的存在来确定受试者的健康状态的测定法。

5. 发明申请

WO2004101816A1 DECOYS FOR NUCLEIC ACID-BINDING PROTEINS 审中-公开
标题翻译：解决核酸结合蛋白
公开(公告)号：WO2004101816A1
公开(公告)日：2004-11-25
申请号：PCT/AU2004/000645
申请日：2004-05-17
申请人： HUMAN GENETIC SIGNATURES PTY LTD , CHRISTENSEN, Ulf, Bech , FLINDT, Esben , KROGSDAM, Ann-Margrethe
发明人： CHRISTENSEN, Ulf, Bech , FLINDT, Esben , KROGSDAM, Ann-Margrethe
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6883 , C12N15/113 , C12N2310/13 , C12N2310/3511
摘要： A decoy in the form of an oligonucleotide or oligonucleotide analogue comprising one or more intercalator pseudonucleotide molecules (IPN), the decoy having a nucleotide target sequence to which a protein is capable of binding.
摘要翻译：包含一种或多种嵌入剂假核苷酸分子（IPN）的寡核苷酸或寡核苷酸类似物形式的诱饵，该诱饵具有蛋白质能够结合的核苷酸靶序列。

6. 发明申请

WO2008113111A1 ASSAY FOR GENE EXPRESSION 审中-公开
标题翻译：测定基因表达
公开(公告)号：WO2008113111A1
公开(公告)日：2008-09-25
申请号：PCT/AU2008/000367
申请日：2008-03-14
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MELIKI, John R. , MILLAR, Douglas Spencer , GRIGG, Geoffrey W.
发明人： MILLAR, Douglas Spencer , GRIGG, Geoffrey W.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6806 , C12Q1/6837 , C12Q1/6846 , C12Q2523/125
摘要： An assay for gene expression comprising treating RNA with an agent such as bisulphate that substantially removes secondary structure of the RNA; and measuring the presence or amount of treated RNA so as to obtain an indication of gene expression. The invention also includes use of oligonucleotide, PNA, LNA or INA probes in the assay.
摘要翻译：一种用于基因表达的测定法，其包括用诸如硫酸氢盐的试剂处理RNA，其基本上除去RNA的二级结构; 并测定经处理的RNA的存在或量，以获得基因表达的指示。本发明还包括在测定中使用寡核苷酸，PNA，LNA或INA探针。

7. 发明申请

WO2007140506A1 MODIFIED MICROBIAL NUCLEIC ACID FOR USE IN DETECTION AND ANALYSIS OF MICROORGANISMS 审中-公开
标题翻译：用于检测和分析微生物的改性微生物核酸
公开(公告)号：WO2007140506A1
公开(公告)日：2007-12-13
申请号：PCT/AU2006/000755
申请日：2006-06-02
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , MELKI, John, R.
发明人： MILLAR, Douglas, Spencer , MELKI, John, R.
IPC分类号： C12Q1/68
CPC分类号： C12Q1/689
摘要： The present invention provides derivatives or modified nucleic acid sequences of several microorganisms for use in the detection and analysis of said microorganisms. The derivative nucleic acids contain the bases adenosine (A), guanosine (G), T (thymine) and U (uracil or some other non-A, G or T base or base-like entity). Given that microbial nucleic acids do not contain methylated cytosine (C) or other C alterations, all C are converted to U. These sequences are amplified where the U in the derivative nucleic acid is replaced by a T, resulting in a modified sequence with the same number of total bases as the corresponding unmodified microbial nucleic acid sequence but made up of a combination of three bases only: A, G and T. As a consequence of this process the nucleic acids derived from the upper and lower strands of the original dsDNA are no longer complementary and the modified microbial sequences have reduced relative genomic complexity for use in detection and analysis of microorganisms.
摘要翻译：本发明提供用于检测和分析所述微生物的几种微生物的衍生物或修饰的核酸序列。衍生的核酸含有腺苷（A），鸟苷（G），T（胸腺嘧啶）和U（尿嘧啶或其他非A，G或T碱基或碱基类实体）。鉴于微生物核酸不含甲基化胞嘧啶（C）或其他C改变，所有C转化为U.这些序列在衍生核酸中的U被T代替时被扩增，导致修饰的序列与与相应的未修饰微生物核酸序列相同数量的总碱基，但由三个碱基的组合组成：A，G和T.作为该方法的结果，源自原始dsDNA的上部和下部链的核酸不再互补，修饰的微生物序列降低了用于微生物检测和分析的相对基因组复杂性。

8. 发明申请

WO2004111229A1 METHYLOMIC ANALYSIS-CYTOSINE CELLULAR SIGNATURES 审中-公开
标题翻译：甲基分析 - 细胞色素细胞标志
公开(公告)号：WO2004111229A1
公开(公告)日：2004-12-23
申请号：PCT/AU2004/000725
申请日：2004-06-01
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , MELKI, John, Robert , MIKLOS, George, L., Gabor
发明人： MILLAR, Douglas, Spencer , MELKI, John, Robert , MIKLOS, George, L., Gabor
IPC分类号： C12N15/09
CPC分类号： C12Q1/6827 , C12Q2523/125
摘要： A method for determining a Cytosine Cellular Signature region in a putative control region of genomic nucleic acid sequence comprising identifying a 5' transcription start site in the sequence; locating at least a first CpG unit upstream or a first CpG unit downstream from the 5' transcription start site; and selecting at least one CpG unit located to form the Cytosine Cellular Signature region.
摘要翻译：一种确定基因组核酸序列的推定对照区域中的胞嘧啶细胞标记区域的方法，包括鉴定序列中的5'转录起始位点; 将至少第一个CpG单元定位在上游或第一个CpG单元下游的5'转录起始位点; 并选择至少一个CpG单元，以形成Cytosine Cellular Signature区域。

9. 发明申请

WO2004096825A1 TREATMENT OF NUCLEIC ACID 审中-公开
标题翻译：治疗核酸
公开(公告)号：WO2004096825A1
公开(公告)日：2004-11-11
申请号：PCT/AU2004/000549
申请日：2004-04-29
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , VOCKLER, Cassandra, Jean , COULSTON, Keralie, Ann
发明人： MILLAR, Douglas, Spencer , VOCKLER, Cassandra, Jean , COULSTON, Keralie, Ann
IPC分类号： C07H21/00
CPC分类号： C07H21/04 , C12Q1/68 , C12Q1/6827 , C12Q2523/125 , C12Q2527/119
摘要： The invention provides methods for treating nucleic acid, particularly nucleic acid that is methylated. In one embodiment, the method can include the steps of (a) providing a denaturing environment to a nucleic acid sample; (b) reacting the nucleic acid sample with a bisulphite reagent and incubating the reaction so as to form a treated nucleic acid sample where methylated nucleotides in the nucleic acid sample remain unchanged while unmethylated nucleotides are converted to another form; (c) diluting the treated nucleic acid sample so as to reduce salt concentration to a level which will not substantially interfere with a nucleic acid precipitating step; (d) precipitating the diluted treated nucleic acid to substantially remove any unwanted reagents or diluents from treated nucleic acid; and (e) carrying out de-sulphonation of the precipitated treated nucleic acid so as to remove sulphonate groups present on the treated nucleic acid so as to obtain a nucleic acid sample substantially free of sulphonate groups.
摘要翻译：本发明提供了用于处理核酸的方法，特别是被甲基化的核酸。在一个实施方案中，该方法可以包括以下步骤：（a）向核酸样品提供变性环境; （b）使核酸样品与亚硫酸氢盐试剂反应并孵育反应以形成处理的核酸样品，其中核酸样品中的甲基化核苷酸保持不变，而未甲基化核苷酸转化为另一种形式; （c）稀释经处理的核酸样品，以将盐浓度降低到基本上不干扰核酸沉淀步骤的水平; （d）沉淀经处理的稀释的核酸，以从处理的核酸中基本上除去任何不想要的试剂或稀释剂; 和（e）进行沉淀的经处理的核酸的脱磺酸以除去存在于经处理的核酸上的磺酸盐基团，以获得基本上不含磺酸盐基团的核酸样品。

10. 发明申请

WO2004065625A1 ASSAY FOR DETECTING METHYLATION CHANGES IN NUCLEIC ACIDS USING AN INTERCALATING NUCLEIC ACID 审中-公开
标题翻译：使用间断核酸检测核酸中甲基化变化的测定
公开(公告)号：WO2004065625A1
公开(公告)日：2004-08-05
申请号：PCT/AU2004/000083
申请日：2004-01-23
申请人： HUMAN GENETIC SIGNATURES PTY LTD , MILLAR, Douglas, Spencer , MELKI, John, Robert , GRIGG, Geoffrey, W. , MIKLOS, George, L., Gabor
发明人： MILLAR, Douglas, Spencer , MELKI, John, Robert , GRIGG, Geoffrey, W. , MIKLOS, George, L., Gabor
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2563/173 , C12Q2563/107 , C12Q2523/125
摘要： A method for detecting the presence of a target nucleic acid in a sample including treating a sample containing nucleic acid with an agent that modifies unmethylated cytosine; providing to the treated sample a detector ligand in the form of an intercalating nucleic acid (INA) capable of binding to a target region of nucleic acid, and allowing sufficient time for the detector ligand to bind to the target nucleic acid; and detecting binding of the detector ligand to nucleic acid molecule in the sample to indicate the presence of the target nucleic acid.
摘要翻译：一种用于检测样品中目标核酸存在的方法，包括用修饰未甲基化胞嘧啶的试剂处理含有核酸的样品; 向经处理的样品提供能够结合核酸靶区的插入核酸（INA）形式的检测剂配体，并且允许足够的时间使检测体配体与靶核酸结合; 并检测检测器配体与样品中核酸分子的结合，以指示靶核酸的存在。

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