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1. 发明申请

WO0227315A3 COMPOUNDS WITH A BRANCHED LINKER 审中-公开
标题翻译：具有分支链接器的化合物
公开(公告)号：WO0227315A3
公开(公告)日：2002-07-11
申请号：PCT/EP0111118
申请日：2001-09-26
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , ANDRES HERBERT , JOSEL HANS-PETER , HOESS EVA , HERRMANN RUPERT , ELTZ HERBERT VON DER
发明人： ANDRES HERBERT , JOSEL HANS-PETER , HOESS EVA , HERRMANN RUPERT , VON DER ELTZ HERBERT
IPC分类号： G01N33/543 , C07F15/00 , C07K1/107 , C07K5/02 , C07K7/02 , C07K14/00 , C07K14/16 , C07K19/00 , C12N15/09 , C12Q1/68 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/547 , G01N33/58 , G01N33/68 , C07K14/18
CPC分类号： G01N33/6845 , C07K1/1075 , C07K1/1077 , C07K14/001 , G01N33/5306 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/68
摘要： The present invention concerns new compounds comprising a branched linker and their use for producing conjugates for applications in diagnostic or therapeutic methods.
摘要翻译：本发明涉及包含支链接头的新化合物及其用于制备用于诊断或治疗方法的缀合物的用途。

2. 发明申请

WO2006015879A1 C-TERMINAL MODIFICATION OF POLYPEPTIDES 审中-公开
标题翻译：多肽的C-末端修饰
公开(公告)号：WO2006015879A1
公开(公告)日：2006-02-16
申请号：PCT/EP2005/008809
申请日：2005-08-12
申请人： ROCHE DIAGNIOSTICS GMBH , F. HOFFMANN-LA ROCHE AG , HOESS, Eva , BORDUSA, Frank , HERRMANN, Rupert , JAKUBKE, Hans-Dieter , TISCHER, Wilhelm
发明人： HOESS, Eva , BORDUSA, Frank , HERRMANN, Rupert , JAKUBKE, Hans-Dieter , TISCHER, Wilhelm
IPC分类号： C12N9/76
CPC分类号： C12N9/6424 , C12N9/6427 , C12P21/06
摘要： The invention relates to a mutated trypsin comprising an amino acid substitution both at position K60 and D189, and at least one more amino acid substitution by histidine at position N143 or position E151. Such trypsin mutant has a preferred cleavage site comprising the amino acids Xaa l - Xaaz His, wherein Xaa l is L, Y or F and Xaa 2 is R or K. The invention also relates to a man-made polypeptide comprising a target peptide and the above cleavage site as well as to a method of producing C-terminally modified target peptides by using this mutated trypsin.
摘要翻译：本发明涉及包含K60和D189位氨基酸取代的突变型胰蛋白酶，以及在N143位或E151位上由组氨酸取代至少一个氨基酸。这种胰蛋白酶突变体具有优选的切割位点，其包含氨基酸Xaa1 -

3. 发明申请

WO1995020764A1 SPECIFIC BINDING SUBSTANCES FOR ANTIBODIES AND THEIR USE FOR IMMUNOASSAYS OR VACCINES 审中-公开
标题翻译：对于抗体特异性结合物质及其用途为了进行免疫和疫苗
公开(公告)号：WO1995020764A1
公开(公告)日：1995-08-03
申请号：PCT/EP1995000333
申请日：1995-01-31
申请人： BOEHRINGER MANNHEIM GMBH , SEIDEL, Christoph , HERRMANN, Rupert , HOESS, Eva , BATZ, Hans-Georg
发明人： BOEHRINGER MANNHEIM GMBH
IPC分类号： G01N33/68
CPC分类号： C07K16/08 , A61K39/00 , C07K1/04 , G01N33/566 , G01N33/56988 , G01N33/6878
摘要： The invention pertains to an immunological binding substance for antibodies and its use in immunoassays and as vaccine. The immunological binding substance, which specifically binds with an antibody specifically directed to an epitope with a natural amino acid sequence and which either bears a detectable marking or is solid-phase bonded or can be bonded to a solid phase via a binding molecule or is bonded to an immunogenic molecule containing a T-cell epitope or to a carrier protein molecule, is characterized in that the specific binding substance constitutes an amino acid sequence corresponding to the natural epitope, wherein the sequence at least to one peptide bond or to one amino acid is changed in that a) one or more -CO-NH peptide binding groups are replaced by a two- or three-atom bridge different from them, b) amino acid side chains are shortened by a -CH2 group or lengthened by a -CH2, -S, -O, -NH, -SO2 or -CO group, c) one or more amino acids are replaced by a nonbiogenic L or D amino acid of the HR'N-Y-COOH type, where Y is a (CH2)n group with n = 2-8 or a -CH-(CH2)m- CR R R group with m = 0-3, where R , R and R , which may be the same or different, are hydrogen, a branched or unbranched C1-C4 alkyl radical or a phenyl, naphthyl or O-. S- or N-containing, 5- or 6-membered heteroaryl radical, which can be methyl, halide, NH2, OH or carboxy substituted, or where Y is a CHR group, where R is a side chain of a natural amino acid in which a -CH2 group is replaced by -S, NH, -CH=, -SO2, -CO, or -O, or in which one or more H atoms are methyl, NH2, carboxyl, SH, halide or hydroxy substituted, where in all cases Y forms a grouping unlike that of any natural amino acid or where R' is methyl, ethyl or phenyl and Y is a CHR group in which R is a side chain of a natural amino acid..
摘要翻译：本发明涉及一种用于抗体的免疫结合物和它们在免疫测定和使用作为疫苗。特异性与特异性针对的表位具有天然氨基酸序列和其或者携带或可检测标记的固相结合，或者可以经由一个特异性结合分子可以结合到固相或免疫原性有效的抗体结合的免疫结合物质，含表位的T细胞分子，或结合于载体蛋白分子，其特征在于特异性结合物质是对应于天然表位的氨基酸序列，其中至少在一个肽键或在氨基酸序列从而改变，使得a）一种或几个-CO-NH肽键的基团被一个不同的两个或三原子桥取代，b）中氨基被-CH 2基团或-CH 2 - ， - S-，-O - ， - NH-，缩短侧链 - SO 2或-CO-基团被扩展，c）一种或多种氨基酸由类型HR'NY-COOH的非生物L或D-氨基酸所取代，其中Y表示（CH 2）n基团，其中n = 2-8或-CH-（CH 2）M-CR <1> [R <2> [R <3> - 基，其中m = 0-3，其中，R <1>，R <2>和R <3>，其可以是相同或不同，表示氢，有支链或无支链的C1-C4 表示烷基或苯基，萘基或一个O，S或含N的代表，其可以被甲基，卤素，NH 2，OH或羧基取代或其中Y代表CHR基的5-6元杂芳基，其中R 表示天然氨基酸的侧链，其中-CH 2基团被-S-，NH - ， - CH = - ， - SO 2 - ， - CO-或-O-取代，或其中一个或多个H原子由甲基取代，NH 2，羧基，SH，卤素或羟基，Y形成在任何天然氨基酸部分的出现在所有情况下，或者其中R“是甲基，乙基或苯基，Y代表CHR基团，其中R为侧链是一种天然氨基酸。

4. 发明申请

WO1994017072A1 NEW BIOTINYLATION REAGENT 审中-公开
标题翻译：新的生物素化
公开(公告)号：WO1994017072A1
公开(公告)日：1994-08-04
申请号：PCT/EP1994000195
申请日：1994-01-25
申请人： BOEHRINGER MANNHEIM GMBH , HUBER, Erasmus , ZINK, Bruno , LENZ, Helmut , HOESS, Eva
发明人： BOEHRINGER MANNHEIM GMBH
IPC分类号： C07D495/04
CPC分类号： C07D495/04 , C12N9/00 , G01N33/532
摘要： A compound has general formula (I), in which Bi is a residue obtained by splitting a carboxyl group from biotin or a biotin derivate; R and R represent independently from each other hydrogen or C1-C4-alkyl; n is an integer from 4 to 10; and X is an alkylene residue substituted by one or several O and/or S atoms, having a 5 to 20 atom long chain. Also disclosed is a conjugate of this compound with a substance having at least one primary and/or secondary amino group NHR .

5. 发明申请

WO2011098400A1 IGF-I POLY (ETHYLENE GLYCOL) CONJUGATES 审中-公开
标题翻译： IGF-I POLY（乙二醇）结合物
公开(公告)号：WO2011098400A1
公开(公告)日：2011-08-18
申请号：PCT/EP2011/051631
申请日：2011-02-04
申请人： F. HOFFMANN-LA ROCHE AG , HESSE, Friederike , HOESS, Eva , MUELLER, Stephanie , TROST-GROSS, Eva Maria
发明人： HESSE, Friederike , HOESS, Eva , MUELLER, Stephanie , TROST-GROSS, Eva Maria
IPC分类号： A61K47/48 , C07K14/65 , C07K5/10
CPC分类号： C07K14/65 , A61K47/48215 , A61K47/60 , C07K1/1075 , C07K5/1019 , C12P21/06
摘要： Herein is reported a method for producing of a polypeptide conjugated to one poly (ethylene glycol) comprising a) providing a nucleic acid encoding an expression construct comprising in 5' to 3' direction a nucleic acid encoding a polypeptide, and a nucleic acid encoding a trypsin site of SEQ ID NO: 01, b) expressing the nucleic acid of a) in a cell and recovering the expression construct from the cell and/or the cultivation medium, c) providing a target peptide with an amino acid sequence of SEQ ID NO: 02 covalently conjugated to a poly (ethylene glycol) at the C-terminal lysine residue, d) incubating the expression construct and the target peptide with the trypsin mutant D189K, K60E, N143H, E151H, and e) recovering and thereby producing the polypeptide conjugated to one poly (ethylene glycol) from the incubation mixture.
摘要翻译：本文报道了一种生产与一种聚（乙二醇）缀合的多肽的方法，包括：a）提供编码包含5'至3'方向的表达构建体的核酸，编码多肽的核酸和编码 SEQ ID NO：01的胰蛋白酶位点，b）在细胞中表达a）的核酸并从细胞和/或培养基中回收表达构建体，c）提供具有SEQ ID NO：01的氨基酸序列的目标肽 NO：02与C末端赖氨酸残基处的聚（乙二醇）共价缀合，d）用胰蛋白酶突变体D189K，K60E，N143H，E151H孵育表达构建体和靶肽，e）回收并由此产生多肽与孵育混合物中的一种聚（乙二醇）缀合。

6. 发明申请

WO0227317A3 CONJUGATES OF DEFINED STOICHIOMETRY 审中-公开
标题翻译：定义STOICHIOMETRY的结合
公开(公告)号：WO0227317A3
公开(公告)日：2003-09-12
申请号：PCT/EP0111101
申请日：2001-09-26
申请人： ROCHE DIAGNOSTICS GMBH , HOFFMANN LA ROCHE , HOESS EVA , ANDRES HERBERT , DONIE FREDERIC , VOGEL RUDOLF , JOSEL HANS-PETER , HERRMANN RUPERT , ELTZ HERBERT VON DER
发明人： HOESS EVA , ANDRES HERBERT , DONIE FREDERIC , VOGEL RUDOLF , JOSEL HANS-PETER , HERRMANN RUPERT , VON DER ELTZ HERBERT
IPC分类号： A61K47/48 , C07K1/107 , G01N30/88 , G01N33/53 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/566 , G01N33/68
CPC分类号： C07K1/1077 , A61K47/643 , A61K47/6889 , G01N33/5306 , G01N33/531 , G01N33/68 , G01N33/6845 , Y10S435/961 , Y10S435/962
摘要： The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 1 and 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomelecule-linker product of uniform stoichiometry in a pre-selected amount.
摘要翻译：本发明涉及一种制备均匀化学计量学的生物分子 - 接头共轭物的方法。其特别涉及由分子量在5kD至500kD之间的生物分子和亲水性接头分子所组成的共轭物，所述接头分子量为1至15kD，4至60个带电荷的残基，其特征在于所述共轭物包含至少一种具有预选量的均匀化学计量的生物分子 - 接头产物。

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