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    • 3. 发明申请
    • MODIFIED AND FUSION ENHANCED ERYTHROCYTES, CELLS AND USES THEREOF
    • 改良和融合增强的ERYTHROCYTES,细胞及其用途
    • WO2010077290A1
    • 2010-07-08
    • PCT/US2009/006459
    • 2009-12-09
    • GLASER, Lawrence, F.
    • GLASER, Lawrence, F.
    • C12N5/00
    • C12N5/0641C12N5/0006C12N2510/00
    • The present invention provides modified fusion enhanced erythrocytes (or other cell types and synthetic cells) which comprise human viral receptor proteins, human viral coreceptor proteins and viral derived proteins capable of mediating entry of respective viruses into the modified erythrocytes, cells or pseudo-cells. The present invention also provides methods of using the fusion enhanced modified erythrocytes, cells or pseudo-cells for the treatment or prevention of viral infections. In one embodiment, the fusion enhanced modified erythrocytes of the present invention comprise CD4 and at least one HIV coreceptor, such as CXCR4 or CCR5 and as well, at least one of cholesterol rafts, fusin, actin, a viral derived protein such as fusion peptide derived from HIV GP 120 or HIV GP41 or a shorter protein derived from a long viral protein, such as a portion of HIV derived GP 120, or HIV GP41 such as the 23 N-terminal peptide of the HIV-I gp 41 protein (AVGIGALFLGFLGAAGSTMGARS) called FP23 (Fusion Peptide). These viral- fusion enhanced cells may also be electrostatic charge enhanced through further additions named in this invention. The minor addition of iron to these modified erythrocytes, in tolerable amouts, yields a greater static charge, per cell, and thus the initial attraction for HIV will be enhanced proffering better initial static bonding to the virion, followed by the strong covalent or hydrophobic bonding which signifies the commencement of the fusion event. The modified erythrocytes, when administered to an HIV patient, bind to the plasma virus and induce the injection of the HIV ribonucleoprotein complex into the cells. The entrapped viral content is sequestered within said cell for at least the period of time that the cell maintains its outer membrane integrity. The virus is thereafter either degraded or deactivated within the erythrocytes, cells or pseudo-cells, or destroyed by erythrophagocytosis.
    • 本发明提供了修饰的融合增强红细胞(或其他细胞类型和合成细胞),其包含能够介导各种病毒进入修饰的红细胞,细胞或假细胞的人类病毒受体蛋白,人类病毒共受体蛋白和病毒衍生蛋白。 本发明还提供了使用融合增强修饰的红细胞,细胞或假细胞来治疗或预防病毒感染的方法。 在一个实施方案中,本发明的融合增强的修饰的红细胞包含CD4和至少一种HIV共受体,例如CXCR4或CCR5,以及胆固醇筏,融合蛋白,肌动蛋白,病毒衍生蛋白如融合肽中的至少一种 衍生自HIV GP120或HIV GP41或来自长病毒蛋白的较短蛋白质,例如HIV衍生的GP120的一部分或HIV GP41,例如HIV-1 gp 41蛋白的23 N-末端肽(AVGIGALFLGFLGAAGSTMGARS )称为FP23(融合肽)。 这些病毒融合增强细胞也可以通过本发明中命名的进一步添加来增强静电电荷。 在可耐受的淀粉中,这些修饰的红细胞轻微添加铁会增加每个细胞的静电荷,从而增强对HIV的初始吸引力,从而提高对病毒粒子的更好的初始静态结合,然后进行强共价或疏水键合 这意味着融合事件的开始。 修饰的红细胞在施用于HIV患者时与血浆病毒结合并诱导将HIV核糖核蛋白复合物注入细胞。 包埋的病毒含量被隔离在所述细胞内至少在细胞保持其外膜完整性的时间段内。 此后,病毒在红细胞,细胞或假细胞内被降解或失活,或被红细胞增多症所破坏。
    • 4. 发明申请
    • TELECOMMUNICATIONS INSTALLATION AND MANAGEMENT SYSTEM AND METHOD
    • 电信安装与管理系统与方法
    • WO9805152A9
    • 2005-10-27
    • PCT/US9713454
    • 1997-07-25
    • GLASER LAWRENCE FSTOWERS BRIAN E
    • GLASER LAWRENCE FSTOWERS BRIAN E
    • H04Q3/545H04L12/24H04M3/00H04M3/22H04M3/533H04Q3/00H04Q3/58H04Q3/62H04M3/50H04M7/14
    • H04L41/022H04M3/22H04M3/533H04Q3/0062H04Q3/625
    • A telecommunications system installation and management system (10) and method for managing, controlling, updating and monitoring telecommunications devices, such as a private branch telephone switch (22, 23), voice messaging system (26), call accounting system (24), central office telephone switch, router, bridge, hub, or associated peripheral equipment. The device is capable of managing and controlling a plurality of different types of telecommunications equipment provided by various different manufacturers to thereby seamlessly integrate the equipment into an easily managed telecommunications system. A local database within the telecommunications system provides for real-time or near real-time access and modification of programming information for the telecommunications equipment and further provides for redundancy in the event of equipment failure. A single point of control for system management and data entry is provided in an integrated application that reduces the amount of necessary data to be entered and that facilitates user modifications to operating parameters. The system preferably actively maintains a live connection with each of the managed devices.
    • 一种用于管理,控制,更新和监视诸如专用分支电话交换机(22,23),语音消息系统(26),呼叫计费系统(24)之类的电信设备的电信系统安装和管理系统(10) 中央办公室电话交换机,路由器,桥接器,集线器或相关的外围设备。 该设备能够管理和控制由各种不同制造商提供的多种不同类型的电信设备,从而将设备无缝地集成到容易管理的电信系统中。 电信系统内的本地数据库提供对电信设备的节目信息的实时或接近实时的访问和修改,并在设备故障的情况下进一步提供冗余。 在集成应用程序中提供了系统管理和数据输入的单一控制点,减少了要输入的必要数据量,并有助于用户修改操作参数。 系统优选地主动地维护与每个被管理设备的实时连接。
    • 7. 发明申请
    • ELECTRICAL CONDUCTOR TERMINAL AND A METHOD OF CONNECTING AN ELECTRICAL CONDUCTOR TO A TERMINAL
    • 电导体端子和将电导体连接到端子的方法
    • WO1998005091A1
    • 1998-02-05
    • PCT/US1997012921
    • 1997-07-28
    • GLASER, Lawrence, F.STOWERS, Brian, E.
    • H01R04/24
    • H01R43/00H01R4/2425H01R4/2429
    • A terminal for connecting electrical conductors which cleaves the electrical conductor as it is connected to the terminal without the use of an additional tool. The terminal (50) includes at least one support post (54) having a slot (62) for receiving an electtrical conductor (80), wherein the electrical conductor is lodged in the slot to create an electrical connection between the conductor and the support post. As the electrical conductor is lodged in the slot, the conductor is cleaved on one side of the slot during the same motion by the installer of lodging in the conductor slot. The terminal also includes a pivotal tab (106) connected to the slot which may be moved by the hands of an installer to release the connection between the conductor and the slot to allow easy removal and replacement of the conductor. The terminal allows the electrical conductors to be installed, cleaved, and removed by the hands of an installer without the use of any tools.
    • 用于连接电导体的端子,其在不使用附加工具的情况下将电导体断开,因为它连接到端子。 端子(50)包括至少一个具有用于容纳电导体(80)的槽(62)的支撑柱(54),其中电导体被放置在槽中以在导体和支撑柱之间产生电连接 。 当电导体被放置在槽中时,导体在插槽的一侧在相同的运动期间由安装者在导体槽中倒伏而被劈开。 端子还包括连接到槽的枢转突片(106),其可以由安装者的手移动以释放导体和槽之间的连接,以便容易地移除和更换导体。 该终端允许电气导体由安装者的手安装,切割和移除,而不使用任何工具。
    • 8. 发明申请
    • A METHOD OF DEVELOPING AN ANTI-PROTEIN AND REGULATION OF A CELLULAR FUNCTION BY ADMINISTERING AN EFFECTIVE AMOUNT OF THE ANTI-PROTEIN
    • 通过施用抗蛋白质的有效量来开发抗蛋白质和调节细胞功能的方法
    • WO03057714A2
    • 2003-07-17
    • PCT/US0241570
    • 2002-12-27
    • GLASER LAWRENCE F
    • GLASER LAWRENCE F
    • A61K35/76A61K38/16A61K39/21C12N5/08C07K
    • A61K38/162C12N2740/16011
    • Alterations to a host cell protein form a set of specific alterations which may be deployed to then limit a trial and error process in order to arrive at an Anti-Protein targeted to a host cell protein. The invention dictates regulation of monomers, multimers, oligomeric subunits and oligomers, or proteins by changing their form and function sufficiently, to yield a new set of interaction rules which closely resemble the rules followed by naturally occurring monomers, multimers, oligomeric subunits oligomers and proteins. This Anti-Protein contains highly specific alterations, which render the ultimate presence and sufficient concentration of these compositions to yield predictably different interaction, structure and function for the cell and which incorporate the necessary coding for transcription, translation and sufficient concentrated production for these Anti-Proteins, to enable regulation of a particular cellular function, such as viral replication.
    • 对宿主细胞蛋白质的改变形成一组具体的改变,其可以部署以限制试验和错误过程,以获得靶向宿主细胞蛋白质的抗蛋白质。 本发明通过改变其形式和功能充分地规定单体,多聚体,低聚亚基和寡聚体或蛋白质的调节,以产生一组新的相互作用规则,其与天然存在的单体,多聚体,寡聚亚单位寡聚体和蛋白质之后的规则非常相似 。 这种抗蛋白质包含高度特异性的改变,这使得这些组合物的最终存在和足够的浓度产生可预期的不同的细胞的相互作用,结构和功能,并且其对于这些抗 - 蛋白质的转录,翻译和足够的浓缩生产 蛋白质,以便能够调节特定的细胞功能,例如病毒复制。