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1. 发明申请

WO9854980A3 CARBOHYDRATE-BASED ENZYME GRANULATES 审中-公开
标题翻译：基于碳水化合物的芝麻籽颗粒
公开(公告)号：WO9854980A3
公开(公告)日：1999-03-04
申请号：PCT/EP9803327
申请日：1998-06-04
申请人： GIST BROCADES NV , BARENDSE RUDOLF CAROLUS MARIA , MEESTERS GABRIEL MARINUS HENRI , HARZ HANS PETER
发明人： BARENDSE RUDOLF CAROLUS MARIA , MEESTERS GABRIEL MARINUS HENRI , HARZ HANS-PETER
IPC分类号： A23K1/00 , A23K1/16 , A23K1/165 , A23K1/18 , B01J2/00 , C12N1/14 , C12N1/15 , C12N9/00 , C12N9/14 , C12N9/16 , C12N9/98 , C12N15/09 , C12N15/55 , C12N15/80 , C12R1/685 , A23K
CPC分类号： C12N15/80 , A23K10/14 , A23K20/163 , A23K20/189 , A23K40/10 , A23K40/20 , A23K40/25 , A23K50/10 , A23K50/40 , A23K50/75 , A23K50/80 , C12N9/16 , C12N9/98 , Y02A40/818
摘要： A process is disclosed for preparing aqueous phytase-containing liquids involving culturing microorganisms of the genus Aspergillus or Trichoderma in a medium containing assimilable carbon and nitrogen sources (e.g.glucose and ammonium ions), filtering the medium and subjecting the resulting filtrate to ultra-filtration to give an aqueous composition having at least 14,000 FTU/g. This aqueous liquid can be used to prepare granulates that can be incorporated in animal feedstuffs.

2. 发明申请

WO9737032A3 PREPARATION OF MICROBIAL POLYUNSATURATED FATTY ACID CONTAINING OIL FROM PASTEURISED BIOMASS 审中-公开
标题翻译：从生物质生物质中制备含微量脂肪酸的含脂肪油
公开(公告)号：WO9737032A3
公开(公告)日：1997-12-31
申请号：PCT/EP9701448
申请日：1997-03-21
申请人： GIST BROCADES NV , BIJL HENDRIK LOUIS , WOLF JOHANNES HENDRIK , SCHAAP ALBERT , VISSER JOHANNES MARTINUS JACOB
发明人： BIJL HENDRIK LOUIS , WOLF JOHANNES HENDRIK , SCHAAP ALBERT , VISSER JOHANNES MARTINUS JACOB
IPC分类号： A23D9/00 , A23D9/02 , A23K1/16 , A23L1/28 , A23L1/30 , A23L1/302 , A61K8/30 , A61K8/36 , A61K8/37 , A61K8/67 , A61K8/92 , A61K8/97 , A61K8/99 , A61Q19/00 , C07C51/47 , C11B1/02 , C11B1/10 , C11C3/00 , C12N1/00 , C12N1/12 , C12N1/14 , C12P7/64 , C12P17/06 , C12P19/42 , C12P23/00 , C12R1/645 , C12R1/89
CPC分类号： C12P23/00 , A23D9/00 , A23K20/158 , A23L31/00 , A23L33/11 , A23L33/12 , A23L33/15 , A23V2002/00 , A61K8/375 , A61K8/67 , A61K8/92 , A61K8/9706 , A61K8/99 , A61K2800/85 , A61Q19/00 , C07C51/47 , C11B1/02 , C11B1/10 , C12N1/005 , C12N1/14 , C12P7/6427 , C12P7/6472 , C12P17/06 , C12P19/42 , C07C57/03 , C07C57/12 , A23V2250/706 , A23V2250/1868
摘要： A microbial polyunsaturated fatty acid (PUFA) - containing oil with a high triglyceride content. A process for obtaining a PUFA-containing oil from a microbial biomass and a process for isolating compounds from a microbial biomass.

3. 发明申请

WO9000601A2 NOVEL GLUCOSE ISOMERASE ENZYMES AND THEIR USE 审中-公开
标题翻译：新葡萄糖异黄酮酶及其使用
公开(公告)号：WO9000601A2
公开(公告)日：1990-01-25
申请号：PCT/EP8900839
申请日：1989-07-17
申请人： GIST BROCADES NV , PLANT GENETIC SYSTEMS NV
发明人： LUITEN RUDOLF GIJSBERTUS MARIE , QUAX WILHELMUS JOHANNES , SCHUURHUIZEN PAUL WILLIAM , MRABET NADIR
IPC分类号： C12N9/02 , C12N9/92 , C12N15/61 , C12P21/02 , C12N11/00
CPC分类号： C12N9/0089 , C12N9/0008 , C12N9/92 , C12P21/02 , C12Y102/01012
摘要： New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.
摘要翻译：提供新的突变葡萄糖异构酶，在施用条件下表现出改善的性质。这些葡萄糖异构酶通过表达编码所述酶的基因获得，所述基因具有至少在一个氨基酸中与野生型葡萄糖异构酶不同的氨基酸序列。优选的突变型酶是来自于流式细胞葡萄糖异构酶的衍生物。

4. 发明申请

WO1980002505A1 COMBINED IN ACTIVATED VACCINE,READY FOR ADMINISTRATION AND PROCESS FOR THE PREPARATION OF SUCH VACCINE,ACTIVE AGAINST EGG PRODUCTION DROP CAUSED BY ADENO LIKE VIRUSES AND AGAINST DISEASES CAUSED BY REO VIRUSES 审中-公开
标题翻译：在活化疫苗中组合，准备用于制备这种疫苗的制剂和方法，对由ADENO类病毒引起的和由抗病毒药物引起的疾病引起的产卵引起的活性
公开(公告)号：WO1980002505A1
公开(公告)日：1980-11-27
申请号：PCT/NL1980000019
申请日：1980-05-21
申请人： GIST BROCADES NV , APONTOWEIL P , KRASSELT M , KOK J
发明人： GIST BROCADES NV
IPC分类号： A61K39/295
CPC分类号： A61K39/235 , A61K39/12 , A61K39/15 , A61K2039/552 , A61K2039/55566 , A61K2039/70 , C12N2710/10034 , C12N2720/12034
摘要： The vaccine is prepared by mixing a reo virus containing liquid, obtained by cultivation of a reo virus, followed by inactivation of this liquid and optionally homogenising it, with a adeno like virus (ALV) containing liquid, obtained by cultivation of an ALV, followed by inactivation of this liquid and optionally homogenising it, and adding the mixture to an oily phase containing as essential components at least one of the components selected from the group consisting of light mineral paraffenic oils, plant oils and naphtenic mineral oils, and one or more emulsifiers, such as non-ionic surface-active compounds derived from alkylene oxide and/or hexahydric alcohols and/or higher natural fatty acids such as esters or ester-ethers optionally followed by homogenising and/or addition of a preservative.
摘要翻译：通过混合通过培养reo病毒获得的含有液体的reo病毒，然后将该液体灭活并任选地使其与含有通过培养ALV获得的含有液体的腺病毒（ALV））均化，制备疫苗，随后通过灭活该液体并任选地使其均化，并将混合物加入到含有作为必需成分的油相中，所述组分选自轻质矿物油，植物油和萘矿物油中的至少一种成分，以及一种或多种乳化剂，例如衍生自环氧烷和/或六元醇的非离子表面活性化合物和/或较高级天然脂肪酸如酯或酯 - 醚，任选地随后均化和/或添加防腐剂。

5. 发明申请

WO9406918A3 TRANSFORMATION OF PHAFFIA RHODOZYMA 审中-公开
标题翻译： PHAFFIA RHODOZYMA的转化
公开(公告)号：WO9406918A3
公开(公告)日：1994-07-07
申请号：PCT/NL9300187
申请日：1993-09-10
申请人： GIST BROCADES NV
发明人： VAN OOYEN ALBERT JOHANNES JOSE
IPC分类号： C12N1/19 , C07K14/39 , C12N1/21 , C12N15/81 , C12P21/02 , C12R1/19 , C12R1/645 , C12N1/16
CPC分类号： C12N15/81 , C07K14/39
摘要： The present invention discloses a transformed Phaffia strain. Also disclosed are specific vectors for transforming Phaffia, preferably such vectors contain expression regulating sequences obtained from Phaffia. The present invention also discloses the use of certain marker genes in Phaffia. The invention further discloses a method for transforming Phaffia.

6. 发明申请

WO9100343A3 MUTANT ENZYME HAVING REDUCED STABILITY UNDER INDUSTRIAL APPLICATION CONDITIONS 审中-公开
标题翻译：在工业应用条件下具有降低稳定性的突变酶
公开(公告)号：WO9100343A3
公开(公告)日：1991-07-11
申请号：PCT/NL9000091
申请日：1990-06-27
申请人： GIST BROCADES NV
发明人： VAN EIJK JAN HENRICUS , QUAX WILHELMUS JOHANNES , SANDERS JOHAN PIETER MARINUS
IPC分类号： C12N15/09 , A21D8/04 , C12N20060101 , C12N1/21 , C12N9/00 , C12N9/28 , C12N9/30 , C12N9/32 , C12N15/52 , C12N15/56 , C12N1/20
CPC分类号： C12N9/2417 , C12N9/00 , C12N15/52
摘要： A mutant enzyme is provided which exhibits reduced stability under industrial application conditions. As an example mutant bacterial α-amylase is provided which has reduced thermostability during baking conditions. This modified α-amylase can be suitably used for improving both loaf volume and crumb softness of bread products without causing any danger of overdextrination.

7. 发明申请

WO8806624A3 MOLECULAR CLONING AND EXPRESSION OF GENES ENCODING PROTEOLYTIC ENZYMES 审中-公开
标题翻译：分子克隆和表达编码蛋白酶的基因
公开(公告)号：WO8806624A3
公开(公告)日：1988-11-17
申请号：PCT/NL8800007
申请日：1988-02-26
申请人： GIST BROCADES NV
发明人： VAN EEKELEN CHRISTIAAN ALBERTU , VAN DER LAAN JOHANNES CORNELIS , MULLENERS LEONARDUS JOHANNES S
IPC分类号： C12N1/20 , C12N1/21 , C12N9/54 , C12N15/00 , C12N15/09 , C12N15/57 , C12N15/75 , C12N15/87 , C12N15/90 , C12R1/07 , C12Q1/68
CPC分类号： C12N15/90 , C12N9/54 , C12N15/75 , C12N15/87
摘要： Novel methods and novel industrial Bacillus strains are provided for enhanced production of serine protease. Plasmid constructs comprising the serine protease gene are introduced into a compatible host, generally a Bacillus already overproducing serine protease. Preferred host cells are those from Bacillus novo sp. PB92 and the preferred serine protease gene also originates from Bacillus PB92. Integration and maintenance of the plasmid in the host cell chromosome can be achieved by including a temperature sensitive origin of replication in the plasmid construct and growing under selective conditions.

8. 发明申请

WO1981001415A1 PROCESS FOR THE PREPARATION OF ACTIVE DRIED MICROORGANISMS 审中-公开
标题翻译：制备活性干燥微生物的方法
公开(公告)号：WO1981001415A1
公开(公告)日：1981-05-28
申请号：PCT/NL1980000038
申请日：1980-11-17
申请人： GIST BROCADES NV , RIET J VAN , BRUIJN J
发明人： GIST BROCADES NV
IPC分类号： C12N01/18
CPC分类号： C12N1/04 , C12N1/005
摘要： Process for the preparation of active dried microorganism mass and in particular active dried baker's yeast, comprising a disintegration of a compressed mass of microorganisms into particles and drying these particles in a fluidized bed drying equipment without previously dried air up to a dry matter content of from 75 to 90%, whereafter the particles of the microorganism mass are additionally treated in a vacuum drum, at a temperature of from 25 to 45`C and a pressure of from 1-10 mm Hg (133-1330 Pa), until the particles have reached a final dry matter content of from 90-98%. Preferably yeast particles are initially dried in a fluidized bed dryer using air of an initial temperature of from 90-130`C and having a space velocity in the bed of 0.8-2.0 m/s, while the particles are kept on a wet-bulb temperature of at most 45`C, and whereby the load of the fluidized bed dryer is 100-1000 kg/m s floor area. More preferably a surface active agent is added to the microorganism mass before drying.
摘要翻译：用于制备活性干燥微生物块，特别是活性干燥面包酵母的方法，包括将压缩的微生物块分解成颗粒并在流化床干燥设备中干燥这些颗粒，而没有预先干燥的空气直到干物质含量为 75至90％，然后在真空鼓中，在25至45℃的温度和1-10mmHg（133-1330Pa）的压力下额外处理微生物块的颗粒，直到颗粒已达到90-98％的最终干物质含量。优选地，酵母颗粒最初在流化床干燥器中使用初始温度为90-130℃的空气干燥，床的空速为0.8-2.0m / s，而颗粒保持在湿球温度最高为45℃，流化床干燥器的负荷为100-1000kg / m 2的占地面积。更优选地，在干燥之前向微生物块中加入表面活性剂。

9. 发明申请

WO9000601A3 NOVEL GLUCOSE ISOMERASE ENZYMES AND THEIR USE 审中-公开
标题翻译：新葡萄糖异黄酮酶及其使用
公开(公告)号：WO9000601A3
公开(公告)日：1990-02-08
申请号：PCT/EP8900839
申请日：1989-07-17
申请人： GIST BROCADES NV , PLANT GENETIC SYSTEMS NV
发明人： LUITEN RUDOLF GIJSBERTUS MARIE , QUAX WILHELMUS JOHANNES , SCHUURHUIZEN PAUL WILLIAM , MRABET NADIR
IPC分类号： C12N9/02 , C12N9/92 , C12N15/61 , C12P21/02 , C12N11/00
CPC分类号： C12N9/0089 , C12N9/0008 , C12N9/92 , C12P21/02 , C12Y102/01012
摘要： New mutant glucose isomerases are provided exhibiting improved properties under application conditions. These glucose isomerases are obtained by expression of a gene encoding said enzyme, having an amino acid sequence which differs at least in one amino acid from the wildtype glucose isomerase. Preferred mutant enzymes are those derived from Actinoplanes missouriensis glucose isomerase.

10. 发明申请

WO8802753A3 9-ALPHA-HYDROXY STEROIDS AND PROCESS FOR THEIR PREPARATION 审中-公开
标题翻译： 9-ALPHA-HYDROXY STEROIDS及其制备方法
公开(公告)号：WO8802753A3
公开(公告)日：1988-06-30
申请号：PCT/NL8700026
申请日：1987-10-09
申请人： GIST BROCADES NV , BATIST JACOBUS NICOLAAS MARIA , MARX ARTHUR FRIEDRICH , ZOEST WILLEM JOHAN VAN , KAPUR JAGDISH CHANDER
发明人： BATIST JACOBUS NICOLAAS MARIA , MARX ARTHUR FRIEDRICH , VAN ZOEST WILLEM JOHAN , KAPUR JAGDISH CHANDER
IPC分类号： C07J1/00 , C07D235/28 , C07D401/12 , C07D413/12 , C07D417/12 , C07J3/00 , C07J5/00 , C07J7/00 , C07J13/00 , C07J21/00 , C07J31/00 , C07J33/00 , C07J41/00 , C07J43/00 , C07J51/00 , A61K31/565 , A61K31/57
CPC分类号： C07D235/28 , C07D401/12 , C07D413/12 , C07D417/12 , C07J1/0011 , C07J1/0014 , C07J1/0033 , C07J1/0048 , C07J5/0053 , C07J7/0045 , C07J7/0055 , C07J7/0085 , C07J13/005 , C07J21/006 , C07J31/006 , C07J41/0038 , C07J41/0094 , C07J43/003
摘要： New 9-alpha-hydroxy steroids are prepared by the introduction of substituents on the D-ring of 9-alpha-hydroxy-androst-4-ene-3,17-dione. The resulting compounds are useful intermediates in the synthesis of corticosteroids.

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