专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2010144283A1 BACILLUS STRAIN FOR INCREASED PROTEIN PRODUCTION 审中-公开
标题翻译：用于增加蛋白质生产的微生物菌株
公开(公告)号：WO2010144283A1
公开(公告)日：2010-12-16
申请号：PCT/US2010/037040
申请日：2010-06-02
申请人： DANISCO US INC. , BONGIORNI, Cristina , FERRARI, Eugenio
发明人： BONGIORNI, Cristina
IPC分类号： C07K14/32 , C12N9/12 , C12N9/16 , C12N1/21
CPC分类号： C12N9/16 , C07K14/32 , C12N9/1205
摘要： The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified Bacillus sp . Host cells that have at least one inactivated phr gene. The enhanced production of proteins of interest by the modified Bacillus sp . Host cells is further increased in modified Bacillus sp . Host cells that overexpress YmaH. Methods for producing proteins of interest in the modified host cells are also provided
摘要翻译：本发明提供已被遗传操作以具有增强的产生感兴趣蛋白质的能力的宿主细胞。特别地，本发明涉及改良的芽孢杆菌属具有至少一个失活的phr基因的宿主细胞。通过改良的芽孢杆菌菌种增强生产的目的蛋白质宿主细胞在改良的芽孢杆菌中进一步增加。宿主细胞过度表达YmaH。还提供了在修饰的宿主细胞中产生目标蛋白质的方法

2. 发明申请

WO2010123754A1 PROTEASES WITH MODIFIED PRO REGIONS 审中-公开
标题翻译：具有修改的PRO区域的PROTEASES
公开(公告)号：WO2010123754A1
公开(公告)日：2010-10-28
申请号：PCT/US2010/031269
申请日：2010-04-15
申请人： DANISCO US INC. , FERRARI, Eugenio , FIORESI, Carol , VAN KIMMENADE, Anita
发明人： FERRARI, Eugenio , FIORESI, Carol , VAN KIMMENADE, Anita
IPC分类号： C12N15/75 , C12N9/54
CPC分类号： C12N9/54 , C12N15/75
摘要： The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
摘要翻译：本发明提供了用于在细菌宿主细胞中生产成熟蛋白酶的方法和组合物。所述组合物包括编码经修饰的蛋白酶的修饰的多核苷酸，所述修饰的多核苷酸在所述亲区具有至少一个突变; 由修饰的多核苷酸编码的修饰的丝氨酸蛋白酶; 表达盒，DNA构建体和包含编码修饰的蛋白酶的修饰的多核苷酸的载体; 和用本发明的载体转化的细菌宿主细胞。所述方法包括用于增强细菌宿主细胞中成熟蛋白酶的产生的方法，例如芽孢杆菌宿主细胞。产生的蛋白酶可用于工业生产酶，适用于各种行业，包括但不限于清洁，动物饲料和纺织加工业。

3. 发明申请

WO2008141281A1 A MODIFIED SECRETION SYSTEM TO INCREASE EXPRESSION OF POLYPEPTIDES IN BACTERIA 审中-公开
标题翻译：增加细菌中多聚糖表达的改良分泌系统
公开(公告)号：WO2008141281A1
公开(公告)日：2008-11-20
申请号：PCT/US2008/063418
申请日：2008-05-12
申请人： DANISCO US INC., GENENCOR DIVISION , VAN KIMMENADE, Anita , FIORESI, Carol , PERES, Caroline , FERRARI, Eugenio
发明人： VAN KIMMENADE, Anita , FIORESI, Carol , PERES, Caroline , FERRARI, Eugenio
IPC分类号： C07K14/32 , C12N15/31 , C12N15/75 , C12P21/02
CPC分类号： C12N15/75
摘要： The present invention provides methods of altering the production of desired polypeptides in a host cell. In particular, the present invention provides polynucleotides encoding truncated SecG proteins capable of facilitating the secretion of desired proteases by a bacterial host cell, such as Bacillus species, as well as expression vectors and a host cell containing the polynucleotides.
摘要翻译：本发明提供了改变宿主细胞中所需多肽的产生的方法。特别地，本发明提供了编码截短的SecG蛋白的多核苷酸，其能够促进由细菌宿主细胞如芽孢杆菌属物种分泌所需的蛋白酶，以及表达载体和含有多核苷酸的宿主细胞。

4. 发明申请

WO2003066818A3 SECRETION, TRANSCRIPTION AND SPORULATION GENES IN BACILLUS CLAUSII 审中-公开
公开(公告)号：WO2003066818A3
公开(公告)日：2003-08-14
申请号：PCT/US2003/003534
申请日：2003-02-06
申请人： GENENCOR INTERNATIONAL, INC. , FERRARI, Eugenio , VAN KIMMENADE, Anita
发明人： FERRARI, Eugenio , VAN KIMMENADE, Anita
IPC分类号： C07H21/02
摘要： The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus . More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.

5. 发明申请

WO2005111203A3 MUTANT a ?MYLASES 审中-公开
标题翻译： MUTANT a？MYLASES
公开(公告)号：WO2005111203A3
公开(公告)日：2006-03-30
申请号：PCT/US2005012074
申请日：2005-04-08
申请人： GENENCOR INT , FERRARI EUGENIO , KOLKMAN MARC , PILGRIM CRAIG E
发明人： FERRARI EUGENIO , KOLKMAN MARC , PILGRIM CRAIG E
IPC分类号： C11D3/386 , C12N9/00 , C12N9/28 , C11D11/00 , C11D17/04 , C12N1/21 , C12N15/31 , C12N15/52
CPC分类号： C12N9/2417 , C11D3/386 , C11D3/38618
摘要： Variant alpha-amylase enzymes are disclosed in which the residues corresponding to R179 and G180 in Bacillus stearothermophilus (SEQ ID NO.3) are deleted. The disclosed variant alpha-amylase enzymes show altered or improved stability and/or activity profiles.
摘要翻译：公开了变体α-淀粉酶，其中相应于嗜热脂肪芽孢杆菌（SEQ ID NO.3）中的R179和G180的残基被缺失。所公开的变体α-淀粉酶显示改变或改善的稳定性和/或活性谱。

6. 发明申请

WO2003066818A2 SECRETION, TRANSCRIPTION AND SPORULATION GENES IN BACILLUS CLAUSII 审中-公开
标题翻译：分泌，转录和运动基因在BACILLUS CLAUSII
公开(公告)号：WO2003066818A2
公开(公告)日：2003-08-14
申请号：PCT/US2003/003534
申请日：2003-02-06
申请人： GENENCOR INTERNATIONAL, INC. , FERRARI, Eugenio , VAN KIMMENADE, Anita
发明人： FERRARI, Eugenio , VAN KIMMENADE, Anita
IPC分类号： C12N
CPC分类号： C07K14/32
摘要： The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus . More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.
摘要翻译：本发明涉及已被遗传操作以具有改变产生表达蛋白质的能力的细胞。特别地，本发明涉及其中引入了外源核酸序列的革兰氏阳性微生物以及在这种宿主细胞中产生蛋白质的方法，例如芽孢杆菌属的成员。更具体地，本发明涉及目的多肽的表达，产生和分泌以及已被遗传操作以具有改变产生表达蛋白的能力的细胞。特别地，本发明提供了通过微生物增强选择的多肽的表达。

7. 发明申请

WO2009120929A1 METHOD FOR AMPLIFYING LOCUS IN BACTERIAL CELL 审中-公开
标题翻译：在细菌中放大位置的方法
公开(公告)号：WO2009120929A1
公开(公告)日：2009-10-01
申请号：PCT/US2009/038511
申请日：2009-03-27
申请人： DANISCO US INC., GENENCOR DIVISION , FERRARI, Eugenio , PERES, Caroline
发明人： FERRARI, Eugenio , PERES, Caroline
IPC分类号： C12N15/75 , C12P21/02
CPC分类号： C12N15/75
摘要： Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A 1 -P-M-A 2 , where A 1 and A 2 are direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.
摘要翻译：本公开的某些方面涉及扩增基因组座位的方法。在某些实施方案中，该方法可以包括：a）使细菌宿主细胞群与必需酶的抑制剂接触，其中细菌宿主细胞包含以下结构的基因组座位：A1-PM-A2，其中A1和A2是直接重复，P包含多肽的编码序列，M包含必需酶的编码序列; 和b）选择抗抑制剂的细胞; 其中对抑制剂具有抗性的细胞具有多个拷贝的扩增单元。

8. 发明申请

WO2009094084A1 ENHANCED PROTEIN PRODUCTION IN BACILLUS 审中-公开
标题翻译：芽孢杆菌增强蛋白质产量
公开(公告)号：WO2009094084A1
公开(公告)日：2009-07-30
申请号：PCT/US2008/087633
申请日：2008-12-19
申请人： DANISCO US INC., GENENCOR DIVISION , CERVIN, Marguerite A. , FERRARI, Eugenio , POWER, Scott D.
发明人： CERVIN, Marguerite A. , FERRARI, Eugenio , POWER, Scott D.
IPC分类号： C12N15/09 , C12N15/67
CPC分类号： C12N15/63 , C07K14/32 , C12N15/75
摘要： The present invention relates to cells that have been genetically manipulated to have an altered capacity to express and/or produce proteins of interest. In particular, the present invention relates to modified host cells of Gram-positive microorganisms, such as Bacillus species that are capable of overexpressing ymaH. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode YmaH, and the modified host cells comprising them. In particular, the present invention relates to compositions and methods of overexpressing YmaH for enhancing the expression and production of proteins of interest (e.g., proteases) in Bacillus species.
摘要翻译：本发明涉及已被遗传操作以具有改变的表达和/或产生感兴趣的蛋白质的能力的细胞。具体而言，本发明涉及能够过表达ymaH的革兰氏阳性微生物的修饰宿主细胞，例如芽孢杆菌属物种。本发明包括含有编码YmaH的多核苷酸序列的多核苷酸构建体和表达载体，以及包含它们的修饰的宿主细胞。特别地，本发明涉及过表达YmaH用于增强芽孢杆菌属物种中感兴趣的蛋白质（例如蛋白酶）表达和产生的组合物和方法。

9. 发明申请

WO03070963A3 IMPROVED PROTEIN EXPRESSION IN BACILLUS SUBTILIS 审中-公开
标题翻译：枯草芽孢杆菌蛋白质表达的改进
公开(公告)号：WO03070963A3
公开(公告)日：2003-10-16
申请号：PCT/US0302961
申请日：2003-01-31
申请人： GENENCOR INT , BRON SIERD , FERRARI EUGENIO , TJALSMA HAROLD
发明人： BRON SIERD , FERRARI EUGENIO , TJALSMA HAROLD
IPC分类号： C07K14/32 , C12N15/75 , C12N15/00 , C07H21/04 , C12N15/09 , C12Q1/68
CPC分类号： C07K14/32 , C12N15/75
摘要： The present invention relates to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention relates to Gram-positive microorganisms having exogenous nucleic acid sequences introduced therein and methods for producing proteins in such host cells, such as members of the genus Bacillus. More specifically, the present invention relates to the expression, production and secretion of a polypeptide of interest and to cells that have been genetically manipulated to have an altered capacity to produce expressed proteins. In particular, the present invention provides for the enhanced expression of a selected polypeptide by a microorganism.
摘要翻译：本发明涉及已被遗传操作以具有改变的产生表达蛋白质的能力的细胞。具体而言，本发明涉及其中引入了外源核酸序列的革兰氏阳性微生物以及在这样的宿主细胞（例如芽孢杆菌属成员）中生产蛋白质的方法。更具体地说，本发明涉及感兴趣的多肽的表达，产生和分泌，并涉及已被遗传操作以产生表达蛋白质的能力发生改变的细胞。特别地，本发明提供了微生物对选定多肽的增强表达。

10. 发明申请

WO2009120929A8 METHOD FOR AMPLIFYING LOCUS IN BACTERIAL CELL 审中-公开
标题翻译：在细菌中放大位置的方法
公开(公告)号：WO2009120929A8
公开(公告)日：2015-10-22
申请号：PCT/US2009038511
申请日：2009-03-27
申请人： DANISCO US INC , FERRARI EUGENIO , PERES CAROLINE
发明人： FERRARI EUGENIO , PERES CAROLINE
IPC分类号： C12N15/75 , C12P21/02
CPC分类号： C12N15/75
摘要： Certain aspects of this disclosure relate to a method of amplifying a genomic locus. In certain embodiments, the method may comprise: a) contacting a population of bacterial host cells with an inhibitor of an essential enzyme, where the bacterial host cells comprise a genomic locus of the structure: A1-P-M-A2, where A1 and A2 are direct repeats, P comprises a coding sequence for a polypeptide, and M comprises a coding sequence for the essential enzyme; and b) selecting for cells that are resistant to the inhibitor; wherein cells that are resistant to the inhibitor have multiple copies of the amplification unit.
摘要翻译：本公开的某些方面涉及扩增基因组座位的方法。在某些实施方案中，该方法可以包括：a）使细菌宿主细胞群与必需酶的抑制剂接触，其中细菌宿主细胞包含以下结构的基因组座位：A1-PM-A2，其中A1和A2为直接重复，P包含多肽的编码序列，M包含必需酶的编码序列; 和b）选择抗抑制剂的细胞; 其中对抑制剂具有抗性的细胞具有多个拷贝的扩增单元。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式