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    • 2. 发明申请
    • PRODUCTION AND USE OF REGULATORY T CELLS
    • 生产和使用调节性T细胞
    • WO2007125362A1
    • 2007-11-08
    • PCT/GB2007/050210
    • 2007-04-24
    • ISIS INNOVATION LTDFENG, GangWOOD, Kathryn, JayneBUSHELL, Andrew, Richard
    • FENG, GangWOOD, Kathryn, JayneBUSHELL, Andrew, Richard
    • C12N5/06
    • C12N5/0636
    • An ex vivo method for generating a population of Treg capable of suppressing rejection of an organ or tissue transplant from a donor animal in a recipient animal, comprises culturing CD4 + T cells from the recipient animal in the presence of IFN-γ plus either donor specific or third-party antigen presenting cells, and harvesting a population of Treg capable of suppressing rejection in the recipient animal. The Treg can be administered, for example intravenously to the recipient, preferably immediately prior to the transplant to suppress transplant rejection. A similar strategy applicable to generating a population of Treg capable of suppressing an autoimmune condition in an animal wherein the animal mounts an immune reaction against an autoantigen, comprises culturing CD4 + T cells from the animal in the presence of cells presenting the autoantigen and IFN-γ and harvesting a population of autoantigen reactive Treg.
    • 用于产生能够抑制受体动物中供体动物的器官或组织移植排斥反应的Treg群体的离体方法包括:在受体动物的存在下培养CD4 + T细胞, IFN-α 加上供体特异性或第三方抗原呈递细胞,以及收获能抑制受体动物排斥反应的Treg群体。 Treg可以例如静脉内施用于接受者,优选在移植前,以抑制移植排斥。 适用于产生能够抑制动物中自身免疫状态的Treg群体的类似策略,其中动物对抗自身抗原产生免疫反应,包括在动物的存在下培养CD4 + T细胞, 呈现自身抗原和IFN- 并收获自身抗原反应性Treg群体。