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    • 1. 发明申请
    • DIAGNOSTIC FOR HEPATITIS VIRUS
    • 诊断病毒性肝炎
    • WO1991014779A1
    • 1991-10-03
    • PCT/JP1991000405
    • 1991-03-28
    • MITSUI TOATSU CHEMICALS, INCORPORATEDTAKADA, AkiraENOMOTO, NobuyukiDATE, TakayasuNAKAO, Toshifumi
    • MITSUI TOATSU CHEMICALS, INCORPORATED
    • C12N15/51
    • C07K14/005C12N2770/24222C12Q1/707
    • The invention has made it possible to diagnose the infection of a patient specimen with a hepatitis C virus (HCV) by amplifying a cDNA, which has been prepared from an RNA extracted and separated from the specimen, by subjecting it to PCR with a specified primer and fractionating the product of PCR by electrophoresis to thereby detect the occurrence of the amplification of a cDNA with a specified sequence and a specified length indicative of the presence of HCV. The invention has made it possible to detect HCV and diagnose the infection of a patient specimen with HCV also by amplifying a cDNA, which has been prepared from an RNA extracted and separated from the specimen, by subjecting it to PCR with a specified primer and detecting the amplifications of a cDNA, which has a specified sequence indicative of the presence of HCV, through hybridization using a probe which selectively hybridizes with said cDNA.
    • 本发明使得可以通过扩增从提取和从样品中提取和分离的RNA制备的cDNA,通过用指定的引物进行PCR来诊断具有丙型肝炎病毒(HCV)的患者标本的感染 并通过电泳对PCR产物进行分级,从而以指定的序列和指定HCV存在的指定长度检测cDNA扩增的发生。 本发明通过扩增从标本提取和分离的RNA制备的cDNA,通过用指定的引物进行PCR检测,可以检测HCV,并用HCV诊断患者标本的感染 通过使用与所述cDNA选择性杂交的探针进行杂交,扩增具有指示HCV存在的指定序列的cDNA。