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1. 发明申请

WO2006047669A9 NON-RANDOM METHOD OF GENE SHUFFLING 审中-公开
公开(公告)号：WO2006047669A9
公开(公告)日：2006-07-20
申请号：PCT/US2005038725
申请日：2005-10-26
申请人： MONSANTO TECHNOLOGY LLC , HAUGE BRIAN M , DONG FENGGAO
发明人： HAUGE BRIAN M , DONG FENGGAO
CPC分类号： C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene families of interest are aligned and inspected for regions of conserved amino acid residues. These conserved regions, preferably of at least 4 (e.g. about 4 to 10) consecutive conserved amino acid residues are candidate regions for the subsequent design of PCR primers to amplify the variable or less conserved regions in between them, followed by non-random reassembly to create a recombinant nucleic acid genetic library of gene family variants.

2. 发明申请

WO2006047669A3 NON-RANDOM METHOD OF GENE SHUFFLING 审中-公开
标题翻译：非随机的基因密封方法
公开(公告)号：WO2006047669A3
公开(公告)日：2006-08-24
申请号：PCT/US2005038725
申请日：2005-10-26
申请人： MONSANTO TECHNOLOGY LLC , HAUGE BRIAN M , DONG FENGGAO
发明人： HAUGE BRIAN M , DONG FENGGAO
IPC分类号： C12N15/10 , C40B40/08 , C40B50/06
CPC分类号： C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene families of interest are aligned and inspected for regions of conserved amino acid residues. These conserved regions, preferably of at least 4 (e.g. about 4 to 10) consecutive conserved amino acid residues are candidate regions for the subsequent design of PCR primers to amplify the variable or less conserved regions in between them, followed by non-random reassembly to create a recombinant nucleic acid genetic library of gene family variants.
摘要翻译：本发明涉及DNA构建体中DNA分子的非随机组装和使用这种构建体的方法，包括产生核酸文库。非随机基因改组优选通过以下步骤完成。首先，任选地，对由相关目的基因家族编码的蛋白质的氨基酸序列进行比对并检查保守氨基酸残基的区域。这些保守区域，优选至少4个（例如约4至10个）连续保守氨基酸残基的保守区域是用于随后设计PCR引物以扩增它们之间的可变或更少保守区域的候选区域，随后是非随机重组至创建基因家族变体的重组核酸遗传文库。

3. 发明申请

WO2008154472A2 METHODS FOR SEQUENCE-DIRECTED MOLECULAR BREEDING 审中-公开
标题翻译：用于顺序指导的分子育种的方法
公开(公告)号：WO2008154472A2
公开(公告)日：2008-12-18
申请号：PCT/US2008/066263
申请日：2008-06-09
申请人： MONSANTO TECHNOLOGY LLC , DOTSON, Stan , DONG, Fenggao , ACHARD, Fred , EATHINGTON, Sam , TAO, Nengbing , MCCUDDIN, Zoe
发明人： DOTSON, Stan , DONG, Fenggao , ACHARD, Fred , EATHINGTON, Sam , TAO, Nengbing , MCCUDDIN, Zoe
IPC分类号： C12N15/82
CPC分类号： A01H5/10 , A01H1/04 , C12Q1/6827 , C12Q1/6874 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B30/02 , G06F19/22
摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
摘要翻译：本发明提供了通过使用直接核酸序列信息来增强植物种质的育种方法和组合物。该方法描述了植物繁殖群体种质中优选核酸序列的鉴定和积累。

4. 发明申请

WO2008154472A3 METHODS FOR SEQUENCE-DIRECTED MOLECULAR BREEDING 审中-公开
标题翻译：用于顺序指导的分子育种的方法
公开(公告)号：WO2008154472A3
公开(公告)日：2009-08-27
申请号：PCT/US2008066263
申请日：2008-06-09
申请人： MONSANTO TECHNOLOGY LLC , DOTSON STAN , DONG FENGGAO , ACHARD FRED , EATHINGTON SAM , TAO NENGBING , MCCUDDIN ZOE
发明人： DOTSON STAN , DONG FENGGAO , ACHARD FRED , EATHINGTON SAM , TAO NENGBING , MCCUDDIN ZOE
IPC分类号： C12Q1/68 , A01H1/04
CPC分类号： G06F19/22 , A01H1/04 , C12Q1/6874 , C12Q1/6895 , C12Q2600/13 , C12Q2600/156 , C40B30/02
摘要： The present invention provides breeding methods and compositions to enhance the germplasm of a plant by the use of direct nucleic acid sequence information. The methods describe the identification and accumulation of preferred nucleic acid sequences in the germplasm of a breeding population of plants.
摘要翻译：本发明提供了通过使用直接核酸序列信息来增强植物种质的育种方法和组合物。该方法描述了植物繁殖群体种质中优选核酸序列的鉴定和积累。

5. 发明申请

WO2006047669A2 NON-RANDOM METHOD OF GENE SHUFFLING 审中-公开
标题翻译：非随机基因组方法
公开(公告)号：WO2006047669A2
公开(公告)日：2006-05-04
申请号：PCT/US2005/038725
申请日：2005-10-26
申请人： MONSANTO TECHNOLOGY LLC , HAUGE, Brian, M. , DONG, Fenggao
发明人： HAUGE, Brian, M. , DONG, Fenggao
CPC分类号： C12N15/1031 , C12N15/1027 , C12N15/1093 , C12N15/64 , C12N15/66
摘要： The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene families of interest are aligned and inspected for regions of conserved amino acid residues. These conserved regions, preferably of at least 4 (e.g. about 4 to 10) consecutive conserved amino acid residues are candidate regions for the subsequent design of PCR primers to amplify the variable or less conserved regions in between them, followed by non-random reassembly to create a recombinant nucleic acid genetic library of gene family variants.
摘要翻译：本发明涉及DNA构建体中DNA分子的非随机组装以及使用这些构建体的方法，包括产生核酸文库。非随机基因改组优选通过以下步骤完成。首先，任选地，由感兴趣的相关基因家族编码的蛋白质的氨基酸序列进行比对并检查保守氨基酸残基的区域。这些保守区域，优选至少4个（例如约4至10个）连续的保守氨基酸残基是候选区域，用于后续设计的PCR引物以扩增它们之间的可变的或较不保守的区域，随后是非随机重组创建基因家族变体的重组核酸基因文库。

6. 发明申请

WO2005021761A1 FATTY ACID DESATURASES FROM PRIMULA 审中-公开
标题翻译：来自PRIMULA的脂肪酸
公开(公告)号：WO2005021761A1
公开(公告)日：2005-03-10
申请号：PCT/US2004/026944
申请日：2004-08-20
申请人： MONSANTO TECHNOLOGY LLC , URSIN, Virginia , FROMAN, Byron , GONZALES, Jennifer , SCREEN, Steven, E. , DONG, Fenggao , LA ROSA, Thomas, J.
发明人： URSIN, Virginia , FROMAN, Byron , GONZALES, Jennifer , SCREEN, Steven, E. , DONG, Fenggao , LA ROSA, Thomas, J.
IPC分类号： C12N15/53
CPC分类号： C12N9/0004 , C12N15/8247 , C12P7/6427
摘要： The invention relates generally to methods and compositions concerning desaturase enzymes that modulate the number and location of double bonds in long chain poly-unsaturated fatty acids (LC-PUFA's). In particular, the invention relates to methods and compositions for improving omega-3 fatty acid profiles in plant products and parts using desaturase enzymes and nucleic acids encoding for such enzymes. In particular embodiments, the desaturase enzymes are Primula Δ6-desaturases. Also provided are improved soybean oil compositions having SDA and a beneficial overall content of omega-3 fatty acids relative to omega-6 fatty acids.
摘要翻译：本发明一般涉及调节长链多不饱和脂肪酸（LC-PUFA）中双键数目和位置的去饱和酶的方法和组合物。特别地，本发明涉及用于使用去饱和酶和编码这些酶的核酸改善植物产品和部分中的ω-3脂肪酸谱的方法和组合物。在具体实施方案中，去饱和酶是Primula Delta6-去饱和酶。还提供了具有SDA的改进的大豆油组合物和相对于ω-6脂肪酸的ω-3脂肪酸的有益总体含量。

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