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1. 发明申请

WO2016106332A1 THERAPEUTIC PARASITE PREPARATION AND METHODS OF MAKING AND USING 审中-公开
标题翻译：治疗PARASITE制剂及其制备和使用方法
公开(公告)号：WO2016106332A1
公开(公告)日：2016-06-30
申请号：PCT/US2015/067408
申请日：2015-12-22
申请人： DONAHUE, Donald, C.
发明人： DONAHUE, Donald, C.
IPC分类号： A61K35/62 , C07K14/435 , C12N5/07
CPC分类号： A61K35/62 , A01K67/033 , A01K2227/703 , A01K2267/0362 , A01K2267/0387 , A61K31/7048 , A61K45/06 , A61K2300/00
摘要： A method of manufacturing a consumable parasitic composition includes: obtaining live parasite from an in vitro cell culture; and combining the live parasite with a consumable composition, the consumable composition having nutrients to maintain the live parasite, such as Hymenolepis diminuta cysticercoids (HDC) or Dipylidium caninum cysticercoids (DCC). The method includes obtaining cleansed Hymenolepis diminuta (HDO); incubating the HDO in cell growth media and fungi and bacteria so as to initiate fungal growth and bacterial growth; washing the HDO with antifungal and antibacterial; cracking the shells with hard beads (e.g., glass) in a swirled solution; filtering the cracked shells for removal of the shells; removing the HDC from cell culture media of the in vitro cell culture; and combining the HDC from the in vitro cell culture with the consumable composition.
摘要翻译：消耗性寄生组合物的制造方法包括：从体外细胞培养获得活体寄生虫; 并且将活体寄生虫与消耗性组合物组合，所述消耗性组合物具有营养物质以维持活体寄生虫，例如隐球菌隐球囊虫（HDC）或犬胆囊囊虫（DCC）。该方法包括获得清洁的Hymenolepis diminuta（HDO）; 在细胞生长培养基和真菌和细菌中孵育HDO以引发真菌生长和细菌生长; 用抗真菌和抗菌洗涤HDO; 用涡旋溶液中的硬珠（例如玻璃）裂化壳; 过滤破壳以去除壳; 从体外细胞培养的细胞培养基中除去HDC; 并将来自体外细胞培养物的HDC与消耗性组合物组合。

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