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1. 发明申请

WO2006128707A8 METHOD OF PRODUCING HAPLOID AND DOUBLED HAPLOID PLANT EMBRYOS 审中-公开
标题翻译：生产HAPLOID和DOUBLED HAPLOID植物胚胎的方法
公开(公告)号：WO2006128707A8
公开(公告)日：2007-11-15
申请号：PCT/EP2006005238
申请日：2006-05-31
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHA , DIRKS ROBERT HELENE GHISLAIN , ANGENENT GERRIT CORNELIS , LELIVELT CECILIA LUCIA CLARA , CUSTERS JOHANNES BERNARDUS MAR
发明人： DIRKS ROBERT HELENE GHISLAIN , ANGENENT GERRIT CORNELIS , LELIVELT CECILIA LUCIA CLARA , CUSTERS JOHANNES BERNARDUS MAR
IPC分类号： A01H1/08 , A01H1/02 , A01H5/00 , A01H5/10
CPC分类号： A01H1/08 , C12N15/8233 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention relates to a method for producing haploid plant embryos, comprising providing microspores or pollen that comprise cell division inducing molecules; pollinating an embryo sac cell, in particular an egg cell, of the plant of which the haploid embryo is to be made with the microspores or pollen; allowing the microspores or pollen to discharge the cell division inducing molecules in or in the vicinity of the embryo sac cell, in particular the egg cell, to trigger division thereof to obtain a haploid plant embryo. When doubled haploid plant embryos are to be produced doubling of the chromosome number takes place at a certain stage after pollination, in particular during cell division or after obtaining the embryo. The invention further relates to the embryos thus obtained, plants regenerated therefrom and progeny thereof.
摘要翻译：本发明涉及一种产生单倍体植物胚胎的方法，包括提供包含细胞分裂诱导分子的小孢子或花粉; 授粉用小孢子或花粉制备单倍体胚的植物的胚囊细胞，特别是卵细胞; 允许小孢子或花粉排出在胚囊细胞，特别是卵细胞中或附近的细胞分裂诱导分子，以引发其分裂以获得单倍体植物胚。当要产生双倍单倍体植物胚胎时，染色体数量的倍增发生在授粉后的特定阶段，特别是在细胞分裂期间或在获得胚胎之后。本发明还涉及由此获得的胚胎，从其再生的植物及其后代。

2. 发明申请

WO2007055573A1 METHOD FOR REMOVING A FLUID FROM A CONTAINER AND DEVICE THEREFOR 审中-公开
标题翻译：从容器及其装置中去除流体的方法
公开(公告)号：WO2007055573A1
公开(公告)日：2007-05-18
申请号：PCT/NL2006/000566
申请日：2006-11-13
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. , DE WIT, Jacobus, Petrus, Cornelis , DIRKS, Robert, Helene, Ghislain , VAN ZON, Edwin , WOUDENBERG, Leendert, Jacobus
发明人： DE WIT, Jacobus, Petrus, Cornelis , DIRKS, Robert, Helene, Ghislain , VAN ZON, Edwin , WOUDENBERG, Leendert, Jacobus
IPC分类号： B01L3/14
CPC分类号： G01N35/1079 , B01L3/5021 , B01L2200/026 , B01L2300/044 , B01L2400/0677 , G01N2035/00495 , Y10T83/39
摘要： The invention relates to a method for removing a liquid, in particular a supernatant, from a container with a sample, comprising of providing a container with a sample consisting of solid constituents and a liquid, arranging an opening in the container, and subsequently centrifuging the container so as to cause the liquid to flow out of the container. The invention further relates to a device for making a container suitable for use in the method.
摘要翻译：本发明涉及一种用于从具有样品的容器中除去液体，特别是上清液的方法，其包括向容器提供由固体成分和液体组成的样品，在容器中布置开口，然后将容器，以使液体从容器流出。本发明还涉及一种用于制造适合于该方法的容器的装置。

3. 发明申请

WO2006128707A1 METHOD OF PRODUCING HAPLOID AND DOUBLED HAPLOID PLANT EMBRYOS 审中-公开
标题翻译：生产HAPLOID和DOUBLED HAPLOID植物胚胎的方法
公开(公告)号：WO2006128707A1
公开(公告)日：2006-12-07
申请号：PCT/EP2006/005238
申请日：2006-05-31
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. , DIRKS, Robert, Helene, Ghislain , ANGENENT, Gerrit Cornelis , LELIVELT, Cecilia, Lucia, Clara , CUSTERS, Johannes, Bernardus, Maria
发明人： DIRKS, Robert, Helene, Ghislain , ANGENENT, Gerrit Cornelis , LELIVELT, Cecilia, Lucia, Clara , CUSTERS, Johannes, Bernardus, Maria
IPC分类号： A01H1/08 , A01H1/02 , A01H5/00 , A01H5/10
CPC分类号： A01H1/08 , C12N15/8233 , C12N15/8261 , C12N15/8287 , Y02A40/146
摘要： The invention relates to a method for producing haploid plant embryos, comprising providing microspores or pollen that comprise cell division inducing molecules; pollinating an embryo sac cell, in particular an egg cell, of the plant of which the haploid embryo is to be made with the microspores or pollen; allowing the microspores or pollen to discharge the cell division inducing molecules in or in the vicinity of the embryo sac cell, in particular the egg cell, to trigger division thereof to obtain a haploid plant embryo. When doubled haploid plant embryos are to be produced doubling of the chromosome number takes place at a certain stage after pollination, in particular during cell division or after obtaining the embryo. The invention further relates to the embryos thus obtained, plants regenerated therefrom and progeny thereof.
摘要翻译：本发明涉及一种产生单倍体植物胚胎的方法，包括提供包含细胞分裂诱导分子的小孢子或花粉; 授粉用小孢子或花粉制备单倍体胚的植物的胚囊细胞，特别是卵细胞; 允许小孢子或花粉排出在胚囊细胞，特别是卵细胞中或附近的细胞分裂诱导分子，以引发其分裂以获得单倍体植物胚。当要产生双倍单倍体植物胚胎时，染色体数量的倍增发生在授粉后的特定阶段，特别是在细胞分裂期间或在获得胚胎之后。本发明还涉及由此获得的胚胎，从其再生的植物及其后代。

4. 发明申请

WO2006094773A3 NEAR REVERSE BREEDING 审中-公开
公开(公告)号：WO2006094773A3
公开(公告)日：2006-09-14
申请号：PCT/EP2006/002095
申请日：2006-03-02
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. , VAN DUN, Cornelis, Maria, Petrus , DIRKS, Robert, Helene, Ghislain
发明人： VAN DUN, Cornelis, Maria, Petrus , DIRKS, Robert, Helene, Ghislain
IPC分类号： C12N15/82
摘要： The present invention relates to a method for producing a homozygous non-human organism from a heterozygous non-human organism, which homozygous organism can be crossed to obtain a hybrid, comprising providing a heterozygous starting organism; allowing the organism to produce SDR-O cells through meiosis, which cells originate from second division restitution; regenerating SDR-O organisms from the SDR-O cells; and producing the homozygous organism from the SDR-O organisms thus obtained. The invention further relates to a method for producing a hybrid, comprising crossing a first homozygous organism that is produced according to the above method with a second homozygous organism. Furthermore, the invention provides homozygous non-human organism and hybrid non-human organisms obtainable by these methods. The invention relates in particular to plants.

5. 发明申请

WO03017753A3 REVERSE BREEDING 审中-公开
标题翻译：反向育种
公开(公告)号：WO03017753A3
公开(公告)日：2003-09-04
申请号：PCT/EP0209526
申请日：2002-08-23
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHA , DIRKS ROBERT HELENE GHISLAIN , VAN DUN CORNELIS MARIA PETRUS , REININK KORNELIUS
发明人： DIRKS ROBERT HELENE GHISLAIN , VAN DUN CORNELIS MARIA PETRUS , REININK KORNELIUS
IPC分类号： A01H1/00 , A01H1/04 , A01H5/00 , C12N15/09 , C12N15/82
CPC分类号： C12N15/01 , A01H1/00 , A01H5/10 , C12N15/8218
摘要： The present invention relates to a method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes; wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.
摘要翻译：本发明涉及一种从杂合非人起始生物有效生产纯合生物的方法，包括提供杂合起始生物; 使起始生物体产生单倍体细胞; 从由此获得的单倍体细胞产生纯合生物; 并选择具有所需染色体组的生物体; 其中在单倍体细胞的产生过程中不发生重组以获得有限数量的遗传上不同的单倍体细胞。也可以防止或抑制重组。

6. 发明申请

WO2003017753A2 REVERSE BREEDING 审中-公开
标题翻译：反向育种
公开(公告)号：WO2003017753A2
公开(公告)日：2003-03-06
申请号：PCT/EP2002/009526
申请日：2002-08-23
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. , DIRKS, Robert, Helene, Ghislain , VAN DUN, Cornelis, Maria, Petrus , REININK, Kornelius
发明人： DIRKS, Robert, Helene, Ghislain , VAN DUN, Cornelis, Maria, Petrus , REININK, Kornelius
IPC分类号： A01H1/04
CPC分类号： C12N15/01 , A01H1/00 , A01H5/10 , C12N15/8218
摘要： The present invention relates to a method for efficiently producing homozygous organisms from a heterozygous non-human starting organism, comprising providing of a heterozygous starting organism; allowing the starting organism to produce haploid cells; creating homozygous organisms from the haploid cells thus obtained; and selecting the organisms having the desired set of chromosomes; wherein during production of the haploid cells no recombination occurs in order to obtain a limited number of genetically different haploid cells. Recombination can also be prevented or suppressed.
摘要翻译：本发明涉及从杂合非人起始生物有效产生纯合生物的方法，包括提供杂合起始生物体; 使起始有机体产生单倍体细胞; 从如此获得的单倍体细胞中产生纯合生物; 并选择具有所需染色体组的生物体; 其中在产生单倍体细胞的过程中不发生重组以获得有限数量的基因不同的单倍体细胞。重组也可以被防止或抑制。

7. 发明申请

WO2007077230A1 RESISTANCE TO PHYSIOLOGICAL DISORDERS IN LETTUCE 审中-公开
标题翻译：抵抗生理障碍
公开(公告)号：WO2007077230A1
公开(公告)日：2007-07-12
申请号：PCT/EP2007/000228
申请日：2007-01-08
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. , VAN DUN, Cornelis, Maria, Petrus , VELTEROP, Joyce, Sylvia , SCHUT, Johan , DIRKS, Robert, Helene, Ghislain
发明人： VAN DUN, Cornelis, Maria, Petrus , VELTEROP, Joyce, Sylvia , SCHUT, Johan , DIRKS, Robert, Helene, Ghislain
IPC分类号： A01H1/00 , A01H5/12
CPC分类号： A01H5/12 , A01H1/04
摘要： The invention relates to a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene- sensitive control under ethylene are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. The invention also relates to the plants thus selected.
摘要翻译：本发明涉及一种与对照植物相比，用于筛选植物群体中存在显示对乙烯易感性降低和生理障碍（特别是Russet Spotting and Yellowing）的个体的方法，其中种子群发芽在黑暗中和在乙烯存在下获得幼苗，当与乙烯下的原始乙烯敏感对照相比具有更长的下胚轴时，选择显示对乙烯和生理紊乱，特别是俄罗斯斑点或黄化的易感性降低的植物。本发明还涉及如此选择的植物。

8. 发明申请

WO2006094774A3 REVERSE PROGENY MAPPING 审中-公开
标题翻译：反向原始映射
公开(公告)号：WO2006094774A3
公开(公告)日：2007-02-15
申请号：PCT/EP2006002096
申请日：2006-03-02
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHA , DIRKS ROBERT HELENE GHISLAIN , SCHUT JOHANNES WILHELMUS
发明人： DIRKS ROBERT HELENE GHISLAIN , SCHUT JOHANNES WILHELMUS
IPC分类号： A01H1/00 , A01H1/04
CPC分类号： A01H1/04 , A01H1/00
摘要： The present invention relates to a method for mapping traits in organisms, in particular in plants. The method according to the present invention comprises a) providing a population of SDR-O organisms, in particular plants, that each arise from one member of a population of unreduced cells resulting from second division restitution, in particular a population of unreduced spores; b) producing SDR-I progeny populations of each of these SDR-O organisms; c) phenotyping the SDR-I progeny populations to identify segregating traits within each SDR-I progeny population; d) if segregating progeny is present in a SDR-I progeny population genotyping the corresponding SDR-O organism and comparing the genotype thereof with the genotype of the other SDR-O organisms to identify heterozygous chromosomal regions associated with the occurrence of the segregating trait identified in the said SDR-I progeny population.
摘要翻译：本发明涉及用于在生物体，特别是植物中的性状测绘的方法。根据本发明的方法包括：a）提供一群SDR-O生物体，特别是植物，它们各自由二分之二的恢复所导致的未减少细胞群的一个成员，特别是未减少的孢子群体产生; b）产生这些SDR-O生物体的SDR-1后代群体; c）对SDR-I后代群体进行表型鉴定，以鉴定每个SDR-I后代群体内的分离性状; d）如果分离后代存在于对相应的SDR-O生物体进行基因分型的SDR-I后代群体中，并将其基因型与其他SDR-O生物的基因型进行比较，以鉴定与所鉴定的分离性状的发生相关的杂合染色体区域在所述SDR-I后代人群中。

9. 发明申请

WO2011144672A1 RESISTANCE TO POST HARVEST DETERIORATION IN CUCUMBER 审中-公开
标题翻译：抵抗在CUCUMBER收获检测
公开(公告)号：WO2011144672A1
公开(公告)日：2011-11-24
申请号：PCT/EP2011/058077
申请日：2011-05-18
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V , DIRKS, Robert, Helene, Ghislain , VAN DUN, Cornelis, Maria, Petrus , VELTEROP, Joyce, Sylvia , KLOET, Johannes, Willem
发明人： DIRKS, Robert, Helene, Ghislain , VAN DUN, Cornelis, Maria, Petrus , VELTEROP, Joyce, Sylvia , KLOET, Johannes, Willem
IPC分类号： A01H5/08
CPC分类号： A01H5/00 , A01H5/08 , A01H5/10
摘要： The invention relates to a cucumber ( Cucumis sativus ) plant, which has the same genetic trait that causes an improved shelf life as found in plants and fruits grown from seeds of cucumber EX5003 representative seeds of which were deposited under NCIMB accession number 41716. The fruits of the cucumber plant have an improved shelf life as compared to the fruits of a control plant that have a normal shelf life, and the plant producing these fruits is obtainable by crossing a cucumber plant with a plant grown from seeds of cucumber EX5003 representative seeds of which were deposited with the NCIMB under NCIMB accession number 41716 and selecting in the F1 progeny of the cross for plants showing an improved shelf life. The invention further relates to progeny and seeds of the plant, to the fruits and to propagation material.
摘要翻译：本发明涉及黄瓜（Cucumis sativus）植物，其具有相同的遗传性状，其导致保存期限如在从NCIMB登录号41716保藏的黄瓜EX5003代表性种子的种子生长的植物和水果中发现的。与具有正常保质期的对照植物的果实相比，黄瓜植物具有更好的保质期，并且通过将黄瓜植物与从黄瓜EX5003的代表种子它们以NCIMB保藏号41716保藏在NCIMB中，并在十字架的F1子代中选择显示改善的保质期的植物。本发明还涉及植物的后代和种子，水果和繁殖材料。

10. 发明申请

WO2008083716A1 RESISTANCE TO PHYSIOLOGICAL DISORDERS IN LETTUCE 审中-公开
标题翻译：抵抗生物学疾病
公开(公告)号：WO2008083716A1
公开(公告)日：2008-07-17
申请号：PCT/EP2007/000229
申请日：2007-01-08
申请人： RIJK ZWAAN ZAADTEELT EN ZAADHANDEL B.V. , VAN DUN, Cornelis, Maria, Petrus , VELTEROP, Joyce, Sylvia , SCHUT, Johan , DIRKS, Robert, Helene, Ghislain
发明人： VAN DUN, Cornelis, Maria, Petrus , VELTEROP, Joyce, Sylvia , SCHUT, Johan , DIRKS, Robert, Helene, Ghislain
IPC分类号： A01G7/00
CPC分类号： A01G7/00
摘要： The invention relates to a method for screening a population of plants for the presence therein of individuals that show a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting and Yellowing, as compared to a control plant, wherein a population of seeds is germinated in darkness and in the presence of ethylene to obtain seedlings that, when having a longer hypocotyl as compared to the original ethylene- sensitive control under ethylene are selected as plants showing a reduced susceptibility to ethylene and physiological disorders, in particular Russet Spotting or Yellowing. The invention also relates to the plants thus selected.
摘要翻译：本发明涉及一种与对照植物相比，用于筛选植物群体中存在显示对乙烯易感性降低和生理障碍，特别是俄罗斯斑点和黄化的个体的方法，其中发芽种子在黑暗中和在乙烯存在下获得幼苗，当与乙烯下的原始乙烯敏感性对照相比具有更长的下胚轴时，选择显示对乙烯和生理紊乱，特别是俄罗斯斑点或黄化的易感性降低的植物。本发明还涉及如此选择的植物。

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