专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO2014011853A3 IGY FROM NOROVIRUS P PARTICLES AND THEIR DERIVATIVES 审中-公开
标题翻译：来自NOROVIRUS P颗粒及其衍生物的IGY
公开(公告)号：WO2014011853A3
公开(公告)日：2014-03-06
申请号：PCT/US2013050044
申请日：2013-07-11
申请人： CHILDRENS HOSP MEDICAL CENTER , DAI YING-CHUN
发明人： DAI YING-CHUN , JIANG XI
IPC分类号： C07K16/08 , A61K39/42 , C12P21/08
CPC分类号： C07K16/02 , A61K2039/505 , C07K16/10
摘要： A method for large-scale production of anti-NoV antibodies for use as a potential treatment for NoV disease using passive immunization. NoV-specific immunoglobulins (IgY) can be produced by immunizing chickens with NoV P particles. The birds continuously produced high titers of antibodies in their eggs for at least 3 months, in which NoV-specific antibody levels reached 4.7-9.2 mg/egg yolk. The egg yolk antibodies strongly reacted with NoV P particles by both ELISA and Western blot and blocked NoV virus-like particle (VLP) and P particle binding to the histo-blood group antigen (HBGA) receptors with a BT50 of about 1 :800. The chicken IgY remain stable at 70°C for 30 min or treatment at pH 4 to 9 for 3 hr, demonstrating that chicken IgY provides large-scale production of anti-NoV antibodies for use in passive immunization against NoV infection.
摘要翻译：用于大规模生产抗NoV抗体的方法，用作使用被动免疫的NoV疾病的潜在治疗。通过用NoV P颗粒免疫鸡可以产生NoV特异性免疫球蛋白（IgY）。鸟类在其卵中连续产生高滴度抗体至少3个月，其中NoV特异性抗体水平达到4.7-9.2mg /蛋黄。蛋黄抗体通过ELISA和Western印迹与NoV病毒样颗粒（VLP）和P颗粒结合到具有约1:800的BT50的组织 - 血型抗原（HBGA）受体而与NoV P颗粒强烈反应。鸡IgY在70℃保持30分钟或在pH4至9下处理3小时，表明鸡IgY提供大规模生产抗NoV抗体用于针对NoV感染的被动免疫。

2. 发明申请

WO2014011853A2 IGY FROM NOROVIRUS P PARTICLES AND THEIR DERIVATIVES 审中-公开
标题翻译： IGY从NOROVIRUS P粒子及其衍生物
公开(公告)号：WO2014011853A2
公开(公告)日：2014-01-16
申请号：PCT/US2013/050044
申请日：2013-07-11
申请人： CHILDREN'S HOSPITAL MEDICAL CENTER , DAI, Ying-Chun
发明人： DAI, Ying-Chun , JIANG, Xi
CPC分类号： C07K16/02 , A61K2039/505 , C07K16/10
摘要： A method for large-scale production of anti-NoV antibodies for use as a potential treatment for NoV disease using passive immunization. NoV-specific immunoglobulins (IgY) can be produced by immunizing chickens with NoV P particles. The birds continuously produced high titers of antibodies in their eggs for at least 3 months, in which NoV-specific antibody levels reached 4.7-9.2 mg/egg yolk. The egg yolk antibodies strongly reacted with NoV P particles by both ELISA and Western blot and blocked NoV virus-like particle (VLP) and P particle binding to the histo-blood group antigen (HBGA) receptors with a BT 50 of about 1 :800. The chicken IgY remain stable at 70°C for 30 min or treatment at pH 4 to 9 for 3 hr, demonstrating that chicken IgY provides large-scale production of anti-NoV antibodies for use in passive immunization against NoV infection.
摘要翻译：用于大规模生产抗NoV抗体的方法用作使用被动免疫的NoV疾病的潜在治疗。 NoV特异性免疫球蛋白（IgY）可以通过用NoV P粒子免疫鸡来产生。鸡在鸡蛋中持续产生高滴度的抗体至少3个月，其中NoV特异性抗体水平达到4.7-9.2mg /蛋黄。蛋黄抗体通过ELISA和Western印迹与NoV P颗粒强烈反应，并且用BT 50蛋白质阻断了NoV病毒样颗粒（VLP）和P颗粒与组织血型抗原（HBGA）大约1：800。鸡IgY在70℃保持稳定30分钟或在pH 4至9处理3小时，表明鸡IgY提供大规模生产的抗NoV抗体用于针对NoV感染的被动免疫。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式