专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

WO1996024661A1 SUBMUCOSA AS A GROWTH SUBSTRATE FOR CELLS 审中-公开
标题翻译：子囊作为细胞的生长基质
公开(公告)号：WO1996024661A1
公开(公告)日：1996-08-15
申请号：PCT/US1996001842
申请日：1996-02-09
申请人： PURDUE RESEARCH FOUNDATION , METHODIST HOSPITAL OF INDIANA, INC. , ELI LILLY AND COMPANY , BADYLAK, Stephen, F. , BODER, George , VOYTIK, Sherry , DEMETER, Robert, J. , CRITSER, John, K. , LIU, Chi
发明人： PURDUE RESEARCH FOUNDATION , METHODIST HOSPITAL OF INDIANA, INC. , ELI LILLY AND COMPANY
IPC分类号： C12N05/00
CPC分类号： C12N5/0655 , C12M23/34 , C12M25/02 , C12N5/0068 , C12N5/0676 , C12N5/0679 , C12N2500/32 , C12N2500/38 , C12N2533/92 , Y10S623/915
摘要： A cell culture growth substrate comprising submucosal tissue of a warm-blooded vertebrate and a method for culturing eukaryotic cells are described. Submucosal tissue used in accordance with the present invention supports the proliferation and differentiation of eukaryotic cells when said cells are contacted with submucosal tissue under conditions conducive to cell proliferation.
摘要翻译：描述了包含温血脊椎动物的粘膜下组织的细胞培养生长底物和培养真核细胞的方法。根据本发明使用的粘膜下组织当所述细胞在有利于细胞增殖的条件下与粘膜下组织接触时，支持真核细胞的增殖和分化。

2. 发明申请

WO2007149847A2 SYSTEMS AND METHODS FOR CRYOPRESERVATION OF CELLS 审中-公开
标题翻译：用于细胞再生的系统和方法
公开(公告)号：WO2007149847A2
公开(公告)日：2007-12-27
申请号：PCT/US2007/071545
申请日：2007-06-19
申请人： WOODS, Erik, John , CRITSER, John, K.
发明人： WOODS, Erik, John , CRITSER, John, K.
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0263 , A01N1/0268 , A01N1/0278
摘要： An auto-nucleating device includes a hollow tube containing a crystalline cholesterol matrix therein. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto- nucleating device is disposed within the vessel and provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within. The opposite end of the vial is initially open to receive the liquid. The end is then sealed to form a closed system for cryopreservation. Another cryopreservation vessel includes an adaptor mounted to a port of a liquid container. The adaptor includes one tubular branch that is closed by a needle septum while another tubular branch includes a barbed fitting for engaging a flexible tube. The flexible term terminates in a needle septum. The container is initially at below-atmospheric pressure. In use, the liquid sample is injected by needle through the septum in the second branch. Once the container is filled with the sample, the flexible tube is heat sealed and severed just above the barbed fitting. The sealed container may then be subject to a cryopreservation protocol. After thawing, the sample liquid may be withdrawn by a needle puncturing the septum in the first branch.
摘要翻译：自动成核装置包括其中含有结晶胆固醇基质的中空管。管的端部由对胆固醇不渗透但对包含在冷冻保存容器中的液体可渗透的膜封闭。自动成核装置设置在容器内并且在冷冻容器内的液体时提供用于冰成核的部位。一个这样的冷冻保存容器是柔性小瓶，其具有适于被针刺穿的一个封闭端口，以抽出其中的液体。小瓶的相对端最初开放以接收液体。然后将其末端密封以形成用于低温保存的封闭系统。另一个冷冻保存容器包括安装到液体容器的端口的适配器。适配器包括一个由针隔膜封闭的管状分支，而另一个管状分支包括用于接合柔性管的带倒钩的配件。灵活的术语终止于针间隔。容器最初处于低于大气压。在使用中，液体样品通过针头穿过第二分支中的隔膜。一旦容器充满样品，柔性管被热封并刚好在倒钩配件上方切断。密封容器然后可以进行冷冻保存方案。解冻后，样品液体可以被穿刺在第一分支中的隔膜的针抽出。

3. 发明申请

WO1998025635A1 SUBMUCOSAL TISSUE INHIBITION OF NEOPLASTIC CELL GROWTH 审中-公开
标题翻译：亚细胞组织抑制神经细胞生长
公开(公告)号：WO1998025635A1
公开(公告)日：1998-06-18
申请号：PCT/US1997022039
申请日：1997-12-10
申请人： PURDUE RESEARCH FOUNDATION , METHODIST HEALTH GROUP, INC. , CRITSER, John, K. , LIU, Chi , DEMETER, Robert, J.
发明人： PURDUE RESEARCH FOUNDATION , METHODIST HEALTH GROUP, INC.
IPC分类号： A61K35/28
CPC分类号： A61K35/38 , A61K35/22 , A61K35/44 , C12N5/0068 , C12N2533/92
摘要： An anti-cancer composition comprising submucosal tissue of warm-blooded vertebrate and a method for inhibiting the growth of undifferentiated eukaryotic cells is described. Submucosal tissue used in accordance with the present invention kills neoplastic eukaryotic cells when said cells are contacted either directly or indirectly with submucosal tissue comprising the tunica submucosa delaminated from both the tunica muscularis and at least the luminal portion of the tunica mucosa of intestinal tissue of a warm-blooded vertebrate.
摘要翻译：描述了包含温血脊椎动物的粘膜下组织的抗癌组合物和抑制未分化真核细胞生长的方法。根据本发明使用的粘膜下组织当所述细胞直接或间接接触粘膜下组织时，杀死肿瘤真核细胞，所述粘膜下组织包括从肌内膜分泌的粘膜下层和至少肠粘膜的腔内部分温血脊椎动物

4. 发明申请

WO2008024575A3 A CRYOPRESERVATION DEVICE AND METHOD 审中-公开
标题翻译： CRYOPRESERVATION设备和方法
公开(公告)号：WO2008024575A3
公开(公告)日：2008-12-11
申请号：PCT/US2007074055
申请日：2007-07-21
申请人： UNIV MISSOURI , CRITSER JOHN K , HAN XU , MA HONGBIN
发明人： CRITSER JOHN K , HAN XU , MA HONGBIN
IPC分类号： C12N1/04
CPC分类号： A01N1/02 , A01N1/0257 , A01N1/0263
摘要： A device and method suitable for the cryopreservation of all types of biological cells is described. In this method, an ultra-fast cooling/warming device system is used to achieve vitrification of individual cells or cell suspensions without cryoprotectant agents (CPA) or with a low concentration of CPAs (
摘要翻译：描述适用于所有类型的生物细胞的低温保存的装置和方法。在该方法中，使用超快速冷却/加温装置系统来实现不使用冷冻保护剂（CPA）或低浓度CPA（<1M）的个体细胞或细胞悬浮液的玻璃化，以减弱细胞内冰晶的形成在冷却过程中形成，并使随后变暖期间的失透最小化。设备系统采用振荡热管（OHP）和纳米流体技术，通过微细加工制成。可以联网几个装置以增加冷冻保存系统可以同时处理的细胞样品的总体积。

5. 发明申请

WO2008128117A1 METHODS FOR DETERMINING OPTIMAL TECHNIQUES FOR VITRIFICATION OF ISOLATED CELLS 审中-公开
标题翻译：用于确定隔离细胞修复的最佳技术的方法
公开(公告)号：WO2008128117A1
公开(公告)日：2008-10-23
申请号：PCT/US2008/060134
申请日：2008-04-11
申请人： THE CURATORS OF THE UNIVERSITY OF MISSOURI OFFICE OF INTELLECTUAL PROPERTY ADMINISTATION , MULLEN, Steven, Francis , CRITSER, John, K. , CHEN, Zi-Jiang
发明人： MULLEN, Steven, Francis , CRITSER, John, K. , CHEN, Zi-Jiang
IPC分类号： A01N1/02 , C12N5/02
CPC分类号： A01N1/02 , A01N1/0221
摘要： A method to optimize a vitrification procedure for suspended cells uses factors such as the physical properties of solutions, the cell permeability to water and permeable cryoprotectants, and the osmotic tolerance of the cells to identify a method to minimize several stresses associated with vitrification procedures.
摘要翻译：优化悬浮细胞的玻璃化过程的方法使用诸如溶液的物理性质，细胞对水的渗透性和可渗透的冷冻保护剂等因素以及细胞的渗透耐受性，以确定最小化与玻璃化过程相关的几种应力的方法。

6. 发明申请

WO2007146253A3 METHODS FOR THE CRYOPRESERVATION OF ANIMAL CELLS THAT CONTAIN HIGH LEVELS OF INTRACELLULAR LIPIDS 审中-公开
公开(公告)号：WO2007146253A3
公开(公告)日：2007-12-21
申请号：PCT/US2007/013727
申请日：2007-06-12
申请人： CRITSER, John, K. , MEN, Hongsheng
发明人： CRITSER, John, K. , MEN, Hongsheng
IPC分类号： C12N5/00 , C12N5/02
摘要： A method for cryopreservation of animal cells with high level of intracellular lipid content, comprises the steps of conducting a delipation procedure using one or more lipolytic agent(s) and/or lipogenesis inhibitors during culture of the animal cells to stimulate the hydrolysis of intracellular lipids to reduce the lipid content, and vitrifying the treated animal cells using a modified vitrification solution and a modified warming solution.

7. 发明申请

WO2007149847A3 SYSTEMS AND METHODS FOR CRYOPRESERVATION OF CELLS 审中-公开
标题翻译：用于细胞再生的系统和方法
公开(公告)号：WO2007149847A3
公开(公告)日：2008-08-14
申请号：PCT/US2007071545
申请日：2007-06-19
申请人： WOODS ERIK JOHN , CRITSER JOHN K
发明人： WOODS ERIK JOHN , CRITSER JOHN K
IPC分类号： C12M3/00
CPC分类号： A01N1/02 , A01N1/0263 , A01N1/0268 , A01N1/0278
摘要： An auto-nucleating device includes a hollow tube containing a crystalline cholesterol matrix therein. The ends of the tube are closed by a membrane that is impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto- nucleating device is disposed within the vessel and provides a site for ice nucleation during freezing of the liquid within the vessel. One such cryopreservation vessel is a flexible vial having a closed port at one adapted to be pierced by a needle to withdraw the liquid within. The opposite end of the vial is initially open to receive the liquid. The end is then sealed to form a closed system for cryopreservation. Another cryopreservation vessel includes an adaptor mounted to a port of a liquid container. The adaptor includes one tubular branch that is closed by a needle septum while another tubular branch includes a barbed fitting for engaging a flexible tube. The flexible term terminates in a needle septum. The container is initially at below-atmospheric pressure. In use, the liquid sample is injected by needle through the septum in the second branch. Once the container is filled with the sample, the flexible tube is heat sealed and severed just above the barbed fitting. The sealed container may then be subject to a cryopreservation protocol. After thawing, the sample liquid may be withdrawn by a needle puncturing the septum in the first branch.
摘要翻译：自动成核装置包括其中含有结晶胆固醇基质的中空管。管的端部由对胆固醇不渗透但对包含在冷冻保存容器中的液体可渗透的膜封闭。自动成核装置设置在容器内并且在冷冻容器内的液体时提供用于冰成核的部位。一个这样的冷冻保存容器是柔性小瓶，其具有适于被针刺穿的一个封闭端口，以抽出其中的液体。小瓶的相对端最初开放以接收液体。然后将其末端密封以形成用于低温保存的封闭系统。另一个冷冻保存容器包括安装到液体容器的端口的适配器。适配器包括一个由针隔膜封闭的管状分支，而另一个管状分支包括用于接合柔性管的带倒钩的配件。灵活的术语终止于针间隔。容器最初处于低于大气压。在使用中，液体样品通过针头穿过第二分支中的隔膜。一旦容器充满样品，柔性管被热封并刚好在倒钩配件上方切断。密封容器然后可以进行冷冻保存方案。解冻后，样品液体可以被穿刺在第一分支中的隔膜的针抽出。

8. 发明申请

WO2008024575A2 A CRYOPRESERVATION DEVICE AND METHOD 审中-公开
标题翻译：一种冷冻保存装置和方法
公开(公告)号：WO2008024575A2
公开(公告)日：2008-02-28
申请号：PCT/US2007/074055
申请日：2007-07-21
申请人： THE CURATORS OF THE UNIVERSITY OF MISSOURI , CRITSER, John K. , HAN, Xu , MA, Hongbin
发明人： CRITSER, John K. , HAN, Xu , MA, Hongbin
IPC分类号： A01N1/02
CPC分类号： A01N1/02 , A01N1/0257 , A01N1/0263
摘要： A device and method suitable for the cryopreservation of all types of biological cells is described. In this method, an ultra-fast cooling/warming device system is used to achieve vitrification of individual cells or cell suspensions without cryoprotectant agents (CPA) or with a low concentration of CPAs (
摘要翻译：描述适于所有类型的生物细胞的低温保存的装置和方法。在该方法中，使用超快速冷却/加热装置系统来实现不含低温保护剂（CPA）或低浓度CPA（<1M）的单个细胞或细胞悬浮液的玻璃化，以减弱细胞内冰的形成在冷却过程中形成晶体，并在随后的加热过程中使反玻璃化最小化。该设备系统采用振荡热管（OHP）和纳米流体技术，并通过微细加工构建。若干设备可联网以增加冷冻保存系统可同时处理的细胞样品的总体积。

9. 发明申请

WO2007146253A2 METHODS FOR THE CRYOPRESERVATION OF ANIMAL CELLS THAT CONTAIN HIGH LEVELS OF INTRACELLULAR LIPIDS 审中-公开
标题翻译：包含高水平细胞内脂肪的动物细胞的CRYOPRESERVING的方法
公开(公告)号：WO2007146253A2
公开(公告)日：2007-12-21
申请号：PCT/US2007013727
申请日：2007-06-12
申请人： CRITSER JOHN K , MEN HONGSHENG
发明人： CRITSER JOHN K , MEN HONGSHENG
IPC分类号： C12N5/08
CPC分类号： A01N1/02 , A01N1/0221
摘要： A method for cryopreservation of animal cells with high level of intracellular lipid content, comprises the steps of conducting a delipation procedure using one or more lipolytic agent(s) and/or lipogenesis inhibitors during culture of the animal cells to stimulate the hydrolysis of intracellular lipids to reduce the lipid content, and vitrifying the treated animal cells using a modified vitrification solution and a modified warming solution.
摘要翻译：一种用于高水平细胞内脂质含量的动物细胞的低温保存方法包括以下步骤：在动物细胞培养期间使用一种或多种脂肪分解剂和/或脂肪生成抑制剂进行熟食过程，以刺激细胞内脂质的水解以降低脂质含量，并使用改良的玻璃化溶液和改良的加温溶液对经处理的动物细胞进行玻璃化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式