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    • 4. 发明申请
    • PLANT PATHOGENESIS-RELATED PROTEINS
    • 植物病原体相关蛋白
    • WO1992020800A1
    • 1992-11-26
    • PCT/EP1992001063
    • 1992-05-14
    • SANDOZ LTD.SANDOZ-PATENT-GMBHCOHEN, YigalGUEGLER, KarlMOESINGER, EgonNIDERMAN, Thierry
    • SANDOZ LTD.SANDOZ-PATENT-GMBH
    • C12N15/29
    • C12N15/8282A01N65/38C07K14/415
    • A family of P14, pathogen related proteins, have been identified in tomato and genes coding for five novel proteins, P14a, P14b, P14d, P14e and P14f have been isolated. It has been found that the proteins encoded by the genes have potent fungicidal activity. Methods of combating fungal disease and fungicidal compositions are provided in which a P14 protein, preferably P14a, P14b, P14c, P14d, P14e or P14f, or a fungicidally active analogue thereof is used as active ingredient. The P14 proteins may be obtained from plant material but the proteins and analogues are preferably prepared by use of recombinant DNA technology. DNA sequences coding for the P14 proteins and analogues, vectors, containing the DNA sequences and host cells transformed with the DNA sequences, as well as processes for production of the protein by culturing the transformed host cells, are provided. In particular transformed plant cells and plants are provided having resistance to fungal disease.
    • 已经在番茄中鉴定了一系列P14,病原体相关蛋白,并分离了编码五种新蛋白的基因,P14a,P14b,P14d,P14e和P14f已被分离。 已经发现,由该基因编码的蛋白具有有效的杀真菌活性。 提供了防治真菌病和杀真菌组合物的方法,其中使用P14蛋白,优选P14a,P14b,P14c,P14d,P14e或P14f或其杀真菌活性类似物作为活性成分。 P14蛋白可以从植物材料获得,但蛋白质和类似物优选通过使用重组DNA技术制备。 提供了编码包含用DNA序列转化的DNA序列和宿主细胞的P14蛋白质和类似物,载体的DNA序列,以及通过培养转化的宿主细胞产生蛋白质的方法。 特别是提供具有抗真菌性疾病的转化的植物细胞和植物。