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    • 2. 发明申请
    • METHOD AND SYSTEM FOR CHARACTERIZING A PIGMENTED BIOLOGICAL TISSUE.
    • 用于表征色素生物组织的方法和系统。
    • WO2008152292A4
    • 2009-02-19
    • PCT/FR2008050911
    • 2008-05-27
    • FORCE ACENTRE NAT RECH SCIENTUNIV PARIS SUDCEROVIC ZORANMOISE NICOLAELATOUCHE GWENDALGOULAS YVES
    • CEROVIC ZORANMOISE NICOLAELATOUCHE GWENDALGOULAS YVES
    • G01N21/64G01N21/27
    • G01N21/64G01N21/274G01N21/645G01N2021/6419G01N2021/6421G01N2021/6491G01N2201/0221G01N2201/062G01N2201/0627
    • Method for determining the content of a chromophoric and nonfluorescent compound, referred to as first compound, of a biological tissue (30) of a biological entity, wherein said biological tissue (30) also comprises a chromophoric and fluorescent compound, referred to as second compound, and wherein said method comprises at least one iteration of the following operations: emission, in the direction of said tissue (30), of a first, "reference", optical radiation and of a second, "measuring", optical radiation, each chosen so as to induce a fluorescence radiation from said second compound, wherein each of said first and second radiations is partially absorbed by said first compound, measurement of said fluorescence radiations induced by said first and second radiations, and determination, from said measurement, of the content of said first compound in said tissue; characterized in that said method also comprises at least one compensation for saturation of the measurement due to too great an absorption of the measuring radiation by said first compound, wherein said compensation comprises a choice, for said measuring radiation, of a wavelength corresponding to a weaker absorption in the absorption spectrum of the first compound.
    • 用于确定生物实体的生物组织(30)的称为第一化合物的发色和非荧光化合物的含量的方法,其中所述生物组织(30)还包含发色和荧光化合物,称为第二化合物 ,并且其中所述方法包括以下操作的至少一次迭代:在所述组织(30)的方向上,发射第一“参考”光辐射和第二“测量”光辐射,其中每个 以便诱导来自所述第二化合物的荧光辐射,其中所述第一和第二辐射中的每一个被所述第一化合物部分吸收,测量由所述第一和第二辐射引起的所述荧光辐射,并且根据所述测量确定 所述第一化合物在所述组织中的含量; 其特征在于,所述方法还包括由于所述第一化合物对测量辐射的吸收太大而对测量饱和的至少一个补偿,其中所述补偿包括对于所述测量辐射选择对应于较弱的波长 吸收第一化合物的吸收光谱。
    • 4. 发明申请
    • DISPOSITIF DE MESURE DES CARACTERISTIQUES D'ABSORPTION LUMINEUSE D'UN ECHANTILLON DE TISSU BIOLOGIQUE
    • 用于测量生物组织样品的光吸收特性的装置
    • WO2003029791A1
    • 2003-04-10
    • PCT/FR2002/002912
    • 2002-08-20
    • CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.)GOULAS, YvesCEROVIC, ZoranMOYA, Ismaël
    • GOULAS, YvesCEROVIC, ZoranMOYA, Ismaël
    • G01N21/31
    • G01N21/3151G01N2021/8466
    • Ce dispositif comporte des moyens (7) d'éclairage de l'échantillon (1), adaptés pour émettre alternativement un premier et un deuxième rayonnements ; des moyens (9) de réception et d'analyse du rayonnement issu de l'échantillon (1) qui comportent des moyens convertisseurs (23) du rayonnement de sortie en signal de contrôle (S 0 ); des moyens de contrôle et de commande (31) émettant des premier (S 1 ) et deuxième (S 2 ) signaux de commande des moyens d'éclairage (7), et un signal de mesure (S m ) ; et des moyens de calcul des caractéristiques d'absorption de l'échantillon (1), en fonction dudit signal de mesure (S m ). Lesdits moyens de contrôle et de commande (31) sont adaptés pour que, sur un intervalle de temps d'intégration (T i ) d'au moins une période (T p ) de variation desdits rayonnements, l'amplitude dudit signal de contrôle (S o ), prise sur les phases d'émission de l'un des rayonnements, soit égale à l'amplitude du signal de contrôle (S o ), prise sur les phases d'émission de l'autre rayonnement, et le signal de mesure (S m ) est significatif de l'un au moins desdits signaux de commande (S 1 , S 2 ).
    • 本发明涉及一种包括用于照亮样品(1)的装置(7)的装置,其被设计为交替地发射第一和第二辐射束; 用于接收和分析来自样本(1)的辐射的装置(9),其包括用于将(23)输出辐射转换成控制信号(S0)的装置; 发射控制照明装置(7)的第一(S1)和第二(S2)信号的监视和控制装置(31)和测量信号(Sm); 以及基于所述测量信号(Sm)计算样品(1)的吸收特性的装置。 所述监视和控制装置(31)适于使得在所述辐射束的变化的至少一个周期(Tp)的积分时间间隔(T i)之后,所述控制信号(S0)的振幅在发射 辐射束之一的相位等于在另一辐射的发射相位上感测的控制信号(S0)的振幅,并且测量信号(Sm)表示所述控制信号(S1,S2)中的至少一个 )。
    • 7. 发明申请
    • METHOD AND SYSTEM FOR CHARACTERIZING A PIGMENTED BIOLOGICAL TISSUE.
    • 用于表征生物生物组织的方法和系统。
    • WO2008152292A1
    • 2008-12-18
    • PCT/FR2008050911
    • 2008-05-27
    • FORCE ACENTRE NAT RECH SCIENTUNIV PARIS SUDCEROVIC ZORANMOISE NICOLAELATOUCHE GWENDALGOULAS YVES
    • CEROVIC ZORANMOISE NICOLAELATOUCHE GWENDALGOULAS YVES
    • G01N21/64G01N21/27
    • G01N21/64G01N21/274G01N21/645G01N2021/6419G01N2021/6421G01N2021/6491G01N2201/0221G01N2201/062G01N2201/0627
    • Method for determining the content of a chromophoric and nonfluorescent compound, referred to as first compound, of a biological tissue (30) of a biological entity, wherein said biological tissue (30) also comprises a chromophoric and fluorescent compound, referred to as second compound, and wherein said method comprises at least one iteration of the following operations: emission, in the direction of said tissue (30), of a first, "reference", optical radiation and of a second, "measuring", optical radiation, each chosen so as to induce a fluorescence radiation from said second compound, wherein each of said first and second radiations is partially absorbed by said first compound, measurement of said fluorescence radiations induced by said first and second radiations, and determination, from said measurement, of the content of said first compound in said tissue; characterized in that said method also comprises at least one compensation for saturation of the measurement due to too great an absorption of the measuring radiation by said first compound, wherein said compensation comprises a choice, for said measuring radiation, of a wavelength corresponding to a weaker absorption in the absorption spectrum of the first compound.
    • 用于确定生物实体的生物组织(30)的发色和非荧光化合物(称为第一化合物)的含量的方法,其中所述生物组织(30)还包含称为第二化合物的发色和荧光化合物 并且其中所述方法包括以下操作的至少一个迭代:在所述组织(30)的方向上发射第一“参考”光学辐射和第二“测量”的光辐射,每个 被选择以便从所述第二化合物诱导荧光辐射,其中所述第一和第二辐射中的每一个被所述第一化合物部分地吸收,由所述第一和第二辐射诱导的所述荧光辐射的测量,以及从所述测量 所述组织中所述第一化合物的含量; 其特征在于,所述方法还包括由于所述第一化合物对测量辐射太大的吸收而对于测量的饱和的至少一个补偿,其中所述补偿包括对于所述测量辐射的对应于较弱的波长的波长的选择 在第一化合物的吸收光谱中的吸收。
    • 8. 发明申请
    • METHOD AND SYSTEM FOR CHARACTERIZING A PIGMENTED BIOLOGICAL TISSUE.
    • 用于表征生物生物组织的方法和系统。
    • WO2008152292A8
    • 2009-04-02
    • PCT/FR2008050911
    • 2008-05-27
    • FORCE ACENTRE NAT RECH SCIENTUNIV PARIS SUDCEROVIC ZORANMOISE NICOLAELATOUCHE GWENDALGOULAS YVES
    • CEROVIC ZORANMOISE NICOLAELATOUCHE GWENDALGOULAS YVES
    • G01N21/64G01N21/27
    • G01N21/64G01N21/274G01N21/645G01N2021/6419G01N2021/6421G01N2021/6491G01N2201/0221G01N2201/062G01N2201/0627
    • Method for determining the content of a chromophoric and nonfluorescent compound, referred to as first compound, of a biological tissue (30) of a biological entity, wherein said biological tissue (30) also comprises a chromophoric and fluorescent compound, referred to as second compound, and wherein said method comprises at least one iteration of the following operations: emission, in the direction of said tissue (30), of a first, "reference", optical radiation and of a second, "measuring", optical radiation, each chosen so as to induce a fluorescence radiation from said second compound, wherein each of said first and second radiations is partially absorbed by said first compound, measurement of said fluorescence radiations induced by said first and second radiations, and determination, from said measurement, of the content of said first compound in said tissue; characterized in that said method also comprises at least one compensation for saturation of the measurement due to too great an absorption of the measuring radiation by said first compound, wherein said compensation comprises a choice, for said measuring radiation, of a wavelength corresponding to a weaker absorption in the absorption spectrum of the first compound.
    • 用于确定生物实体的生物组织(30)的发色和非荧光化合物(称为第一化合物)的含量的方法,其中所述生物组织(30)还包含称为第二化合物的发色和荧光化合物 并且其中所述方法包括以下操作的至少一个迭代:在所述组织(30)的方向上发射第一“参考”光学辐射和第二“测量”的光辐射,每个 被选择以便从所述第二化合物诱导荧光辐射,其中所述第一和第二辐射中的每一个被所述第一化合物部分地吸收,由所述第一和第二辐射诱导的所述荧光辐射的测量,以及从所述测量 所述组织中所述第一化合物的含量; 其特征在于,所述方法还包括由于所述第一化合物对测量辐射太大的吸收而对于测量的饱和的至少一个补偿,其中所述补偿包括对于所述测量辐射的对应于较弱的波长的波长的选择 在第一化合物的吸收光谱中的吸收。