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1. 发明申请

WO2009128892A2 FLEXIBLE MEMBRANE VALVE FOR CELL CULTURE VESSEL 审中-公开
标题翻译：用于细胞培养血管的柔性膜片
公开(公告)号：WO2009128892A2
公开(公告)日：2009-10-22
申请号：PCT/US2009002302
申请日：2009-04-14
申请人： CORNING INC , CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
发明人： CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
IPC分类号： C12M1/24
CPC分类号： C12M37/04 , C12M23/08 , C12M23/10 , C12M23/24 , C12M23/26 , C12M23/38 , C12M37/02
摘要： An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold. The filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter.
摘要翻译：用于培养细胞的组件包括限定用于容纳细胞培养基的储存器和与储存器流体连通的开口的壳体。细胞培养组件还包括被配置为密封地接合开口的过滤阀组件。过滤阀组件包括柔性膜，邻接侧壁，端壁和微生物过滤器。端壁具有开口并且延伸穿过侧壁的一端以在侧壁和端壁内形成主腔。柔性膜具有横跨膜的狭缝，并且在主空腔内是一次性的，使得膜将主腔密封地划分为（i）形成在端壁和膜之间的腔室和（ii）由膜形成的小腔和侧壁的一部分。小空腔与储存器流体连通。狭缝被偏压在关闭位置，以防止流体从小空腔进入腔室，并被构造成打开，以允许气体在薄膜上的压差高于阈值时从小腔进入腔室。过滤器设置在膜和开口之间，使得流过端壁中的开口进入室的空气通过过滤器。

2. 发明申请

WO2009128894A2 FLOAT VALVE FOR CELL CULTURE VESSEL 审中-公开
标题翻译：细胞培养皿用浮球
公开(公告)号：WO2009128894A2
公开(公告)日：2009-10-22
申请号：PCT/US2009002304
申请日：2009-04-14
申请人： CORNING INC , CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
发明人： CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
IPC分类号： C12M1/24
CPC分类号： C12M23/08 , C12M23/56 , C12M29/20 , C12M37/02
摘要： A cell culture vessel includes a housing defining a reservoir, an inlet and an outlet in fluid communication with the reservoir, a valve assembly, and an antimicrobial filter. The valve assembly includes (i) a side wall defining a passageway for fluid flow between the reservoir and the outlet, (ii) a floating element disposed in the passageway and configured to float on the culture medium, (iii) a stop feature configured to sealingly engage the floating element to prevent the culture medium from flowing from the reservoir through the outlet, and (iv) a capture feature configured to retain the floating element within the passageway and to allow fluid to flow from the reservoir through the passageway. The microbial filter is positioned such that air flowing in the outlet to the passageway passes through the filter.
摘要翻译：细胞培养容器包括限定储存器的壳体，与储存器流体连通的入口和出口，阀组件和抗微生物过滤器。阀组件包括（i）限定通道的侧壁，用于在储存器和出口之间流体流动，（ii）浮置元件，其设置在通道中并且构造成浮在培养基上，（iii）停止特征，密封地接合浮动元件以防止培养基从储存器通过出口流动，以及（iv）捕获特征构造成将浮动元件保持在通道内并允许流体从储存器流过通道。微生物过滤器被定位成使得在出口处流到通道的空气通过过滤器。

3. 发明申请

WO2009148512A2 ASSEMBLY OF CELL CULTURE VESSELS 审中-公开
标题翻译：细胞培养血管的组装
公开(公告)号：WO2009148512A2
公开(公告)日：2009-12-10
申请号：PCT/US2009003127
申请日：2009-05-20
申请人： CORNING INC , CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
发明人： CATTADORIS HENRY J , MARTIN GREGORY R , TANNER ALLISON J
IPC分类号： C12M1/00 , C12M1/14
CPC分类号： B29C65/1629 , B29C45/0053 , B29C45/16 , B29C65/08 , B29C65/14 , B29C65/1412 , B29C65/1454 , B29C65/1477 , B29C65/1616 , B29C65/1654 , B29C65/1664 , B29C65/1674 , B29C65/4815 , B29C65/4855 , B29C66/112 , B29C66/1122 , B29C66/114 , B29C66/5346 , B29C66/542 , B29C66/71 , B29C66/712 , B29C66/7352 , B29C2035/0822 , B29C2045/0079 , B29K2009/06 , B29K2023/00 , B29K2023/06 , B29K2023/083 , B29K2023/12 , B29K2025/00 , B29K2027/06 , B29K2027/12 , B29K2027/18 , B29K2033/12 , B29K2067/00 , B29K2069/00 , B29K2077/00 , B29K2081/06 , B29K2995/0065 , B29K2995/0069 , C12M23/08 , C12M23/20 , C12M23/24 , C12M23/44 , B29K2083/005 , B29K2025/08 , B29K2025/06 , B29K2023/38 , B29K2023/18 , B29K2023/14 , B29K2023/0616
摘要： A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.
摘要翻译：细胞培养装置包括由第一主表面，与第一主表面间隔开的相对的第二主表面形成的细胞培养室，以及围绕第一腔室并在第一和第二主表面之间延伸的第一侧壁。靠近第一主表面的第一侧壁的一部分包括红外吸收材料，并且靠近第二主表面的第一侧壁的部分由基本上非红外吸收材料形成。第一主表面是由细胞培养液不渗透的透气性聚合物膜形成的。

4. 发明申请

WO2009128894A3 FLOAT VALVE FOR CELL CULTURE VESSEL 审中-公开
公开(公告)号：WO2009128894A3
公开(公告)日：2009-10-22
申请号：PCT/US2009/002304
申请日：2009-04-14
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J. , MARTIN, Gregory, R , TANNER, Allison, J
发明人： CATTADORIS, Henry, J. , MARTIN, Gregory, R , TANNER, Allison, J
IPC分类号： C12M1/00
摘要： A cell culture vessel includes a housing defining a reservoir, an inlet and an outlet in fluid communication with the reservoir, a valve assembly, and an antimicrobial filter. The valve assembly includes (i) a side wall defining a passageway for fluid flow between the reservoir and the outlet, (ii) a floating element disposed in the passageway and configured to float on the culture medium, (iii) a stop feature configured to sealingly engage the floating element to prevent the culture medium from flowing from the reservoir through the outlet, and (iv) a capture feature configured to retain the floating element within the passageway and to allow fluid to flow from the reservoir through the passageway. The microbial filter is positioned such that air flowing in the outlet to the passageway passes through the filter.

5. 发明申请

WO2009148504A1 CELL CULTURE APPARATUS AND METHOD 审中-公开
标题翻译：细胞培养装置及方法
公开(公告)号：WO2009148504A1
公开(公告)日：2009-12-10
申请号：PCT/US2009/003108
申请日：2009-05-20
申请人： CORNING INCORPORATED , CATTADORIS, Henry J. , POPOLOSKI, Martin J. , TANNER, Allison J.
发明人： CATTADORIS, Henry J. , POPOLOSKI, Martin J. , TANNER, Allison J.
IPC分类号： C12M1/00 , B01F11/00
CPC分类号： C12M23/14 , B01F11/0065 , C12M23/34 , C12M35/04
摘要： A cell culture apparatus includes a housing(10), a bag(50a-e) and an inflatable bladder (40). The bag is disposed in the housing and has an interior surface defining a chamber for culturing cells. The bladder is disposed in the housing, external to the bag, and is sufficiently inflatable and expandable to exert pressure on the bag when the chamber is substantially free of fluid. The bladder may be inflated and expanded to reduce movement of the bag within the housing. Alternatively, or in addition, the bladder may be inflated and expanded to apply a mechanical stress on cells cultured within the chamber of the bag.
摘要翻译：细胞培养装置包括壳体（10），袋（50a-e）和可膨胀囊（40）。袋子设置在壳体中并具有限定用于培养细胞的室的内表面。囊被布置在壳体的外部，并且当腔室基本上没有流体时，其充分膨胀和膨胀以对袋施加压力。囊可以膨胀和膨胀以减少袋在壳体内的运动。或者或另外，膀胱可以膨胀和膨胀，以将机械应力施加到在袋的腔室内培养的细胞上。

6. 发明申请

WO2009128892A3 FLEXIBLE MEMBRANE VALVE FOR CELL CULTURE VESSEL 审中-公开
公开(公告)号：WO2009128892A3
公开(公告)日：2009-10-22
申请号：PCT/US2009/002302
申请日：2009-04-14
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
发明人： CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
IPC分类号： C12M1/12 , C12M1/22 , C12M1/24
摘要： An assembly for culturing cells includes a housing defining a reservoir for containing cell culture media and an opening in fluid communication with the reservoir. The cell culture assembly further includes a filter valve assembly configured to sealingly engage the opening. The filter valve assembly includes a flexible membrane, a contiguous side wall, an end wall, and a microbial filter. The end wall has an opening and extends across one end of the side wall to form a major cavity within the side wall and the end wall. The flexible membrane has a slit spanning the membrane and is disposable within the major cavity such that the membrane sealingly divides the major cavity into (i) a chamber formed between the end wall and the membrane and (ii) a minor cavity formed by the membrane and a portion of the side wall. The minor cavity is in fluid communication with the reservoir. The slit is biased in a closed position to prevent fluid from passing from the minor cavity into the chamber and is configured to open to allow gas to pass from the minor cavity into the chamber when the pressure differential across the membrane is above a threshold. The filter is disposed between the membrane and the opening such that air flowing through the opening in the end wall into the chamber passes through the filter.

7. 发明申请

WO2009148512A3 ASSEMBLY OF CELL CULTURE VESSELS 审中-公开
公开(公告)号：WO2009148512A3
公开(公告)日：2009-12-10
申请号：PCT/US2009/003127
申请日：2009-05-20
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
发明人： CATTADORIS, Henry, J. , MARTIN, Gregory, R. , TANNER, Allison, J.
IPC分类号： B29C65/14 , C12M1/14
摘要： A cell culture apparatus includes a cell culture chamber formed by a first major surface, an opposing second major surface spaced apart from the first major surface, and a first side wall around the first chamber and extending between the first and second major surfaces. A portion of the first sidewall proximate the first major surface comprises an infrared absorbent material, and a portion the first sidewall proximate the second major surface is formed from substantially non-infrared absorbent material. The first major surface is formed by a gas permeable polymeric film that is impermeable to cell culture liquid.

8. 发明申请

WO2006019836A1 CULTURE FLASK 审中-公开
标题翻译：文化大厦
公开(公告)号：WO2006019836A1
公开(公告)日：2006-02-23
申请号：PCT/US2005/024887
申请日：2005-07-15
申请人： CORNING INCORPORATED , CATTADORIS, Henry, J.
发明人： CATTADORIS, Henry, J.
IPC分类号： C12M1/24
CPC分类号： C12M23/08 , B01L3/502 , C12M23/00
摘要： The present invention provides a flask having a top piece (20) and a bottom piece (22) with optical properties on the interior surface (24) of which cells are grown. The top piece (20) and the bottom piece (22) are joined by a removable elastomeric seal (26). By disengaging the elastomeric seal (26) from the flask body (12), the top piece (20) can be separated from the bottom piece (22) providing access to the flask interior.
摘要翻译：本发明提供一种烧瓶，其具有在细胞生长的内表面（24）上具有光学性质的顶部件（20）和底部件（22）。顶部件（20）和底部件（22）通过可拆卸的弹性密封件（26）连接。通过使弹性密封件（26）与烧瓶主体（12）脱离接合，顶部件（20）可与底部件（22）分开，从而提供通向烧瓶内部的通路。

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