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    • 1. 发明申请
    • BACTERIAL SYSTEM FOR PROTEIN TRANSPORT IN EUKARYOTIC CELLS
    • 细菌SYSTEM FOR蛋白转运到真核细胞
    • WO2005085417A3
    • 2006-01-12
    • PCT/DE2005000330
    • 2005-02-26
    • UNIV HALLE WITTENBERGBONAS ULLABUETTNER DANIELA
    • BONAS ULLABUETTNER DANIELA
    • C07K14/195C12N15/62C12N15/74C12N1/21C12R1/64
    • C07K14/195C07K2319/036
    • The aim of the invention is to develop a system for the targeted transport of proteins into eukaryotic cells. The specific transport of proteins into eukaryotic cells is carried out using a type III secretion system, using bacteria strains that are mutated in hpaB or homogenous genes. A non-pathogenic strain is generated from a Gram-negative pathogenic bacteria strain, said non-pathogenic strain being mutated in the hpaB-gen or a homogenous gene which does not mediate any significant translocation of bacterial effector proteins. Over-expressed effector proteins or proteins containing the type 111 secretion signal of a non-effector protein can, however, be translocated into eukaryotic cells using the bacterially inherent TTSS. The inventive bacterial system is used to transport bacterial proteins into eukaryotic cells, in order to influence or modify cellular processes such as gene expression, growth, development and defence/resistance mechanisms.
    • 本发明基于开发一种系统,用于在真核细胞中的蛋白质的靶向递送的问题。 蛋白质在真核细胞中的特定于传输方式是通过使用III型分泌系统使用在HPAB或同源基因的突变的细菌菌株进行。 从革兰氏阴性致病细菌菌株开始是一种非致病性菌株产生,它们分别在HPAB基因。 突变同源基因,这给细菌效应蛋白的无显著易位。 具有非效应物的类型111分泌的,但可以通过在真核细胞利用细菌自身的TTSS易位过表达或效应蛋白。 该新型细菌系统用于输送细菌蛋白到真核细胞,以影响细胞过程,如基因表达,生长,电阻或修改的发展和防御机制。