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1. 发明申请

WO2012083297A3 ADENOVIRAL VECTORS WITH MODIFIED HEXON REGIONS 审中-公开
公开(公告)号：WO2012083297A3
公开(公告)日：2012-06-21
申请号：PCT/US2011/065753
申请日：2011-12-19
申请人： GENVEC, INC. , BRUDER, Joseph, T. , BROUGH, Douglas, E.
发明人： BRUDER, Joseph, T. , BROUGH, Douglas, E.
IPC分类号： C07K14/075 , C12N15/861
摘要： The invention provides a nucleic acid sequence encoding a chimeric adenovirus hexon protein, wherein the chimeric hexon protein comprises a first portion and a second portion. The first portion comprises at least 10 contiguous amino acid residues from a first adenovirus serotype (e.g., serotype 5 adenovirus hexon protein), optionally with one amino acid substitution. The second portion comprises (a) at least one hypervariable region (HVR) of a hexon protein of an adenovirus of a second adenovirus serotype, or (b) at least one synthetic hypervariable region (HVR) that is not present in the hexon protein of the wild-type adenovirus of the first adenovirus serotype.

2. 发明申请

WO2007073513A2 METHOD FOR PROPAGATING ADENOVIRAL VECTORS ENCODING INHIBITORY GENE PRODUCTS 审中-公开
标题翻译：用于传播编码基因产物的腺病毒载体的方法
公开(公告)号：WO2007073513A2
公开(公告)日：2007-06-28
申请号：PCT/US2006/060732
申请日：2006-11-08
申请人： GENVEC, INC. , GALL, Jason, G., D. , BROUGH, Douglas, E. , RICHTER, King, C.
发明人： GALL, Jason, G., D. , BROUGH, Douglas, E. , RICHTER, King, C.
IPC分类号： C12N15/09
CPC分类号： C12N15/635 , C12N7/00 , C12N15/86 , C12N2710/10343 , C12N2710/10352 , C12N2830/006
摘要： The invention provides a method of propagating an adenoviral vector. The method comprises (a) providing a cell comprising a cellular genome comprising a nucleic acid sequence encoding a tetracycline operon repressor protein (tetR), and (h) contacting the cell with an adenoviral vector comprising a heterologous nucleic acid sequence encoding a toxic protein. The heterologous nucleic acid sequence is operably linked to a promoter and one or more tetracycline operon operator sequences (tetO), and expression of the heterologous nucleic acid sequence is inhibited in the presence of tetR, such that the adenoviral vector is propagated. The invention also provides a system comprising the aforementioned cell and adenoviral vector.
摘要翻译：本发明提供了一种繁殖腺病毒载体的方法。该方法包括（a）提供包含细胞基因组的细胞，所述细胞基因组包含编码四环素操纵子阻遏蛋白（tetR）的核酸序列，和（h）使细胞与包含编码毒性蛋白质的异源核酸序列的腺病毒载体接触。异源核酸序列可操作地连接到启动子和一个或多个四环素操纵子操纵子序列（tetO），并且异源核酸序列的表达在tetR存在下被抑制，使得腺病毒载体被繁殖。本发明还提供了包含上述细胞和腺病毒载体的系统。

3. 发明申请

WO2004076626A2 MATERIALS AND METHODS FOR TREATING DISORDERS OF THE EAR 审中-公开
标题翻译：用于治疗耳的病症的材料和方法
公开(公告)号：WO2004076626A2
公开(公告)日：2004-09-10
申请号：PCT/US2004/004891
申请日：2004-02-19
申请人： GENVEC, INC. , BROUGH, Douglas, E.
发明人： BROUGH, Douglas, E.
IPC分类号： C12N
CPC分类号： A61K38/17 , A61K38/00 , A61K48/005 , C07K14/4702 , C12N7/00 , C12N15/86 , C12N2501/60 , C12N2710/10343
摘要： The invention is directed to a method of changing the sensory perception of an animal. The method comprises administering an expression vector comprising a nucleic acid sequence encoding an atonal-associated factor, which is expressed to produce the atonal-associated factor resulting in generation of hair cells that allow perception of stimuli in the inner ear. Also provided is a method of generating a hair cell in differentiated sensory epithelia in vivo. The method comprises contacting differentiated sensory epithelial cells with an adenoviral vector (a) deficient in one or more replication-essential gene functions of the El region and E4 region, (b) comprising a spacer in the E4 region, and (c) comprising a nucleic acid sequence encoding an atonal-associated factor. The nucleic acid sequence is expressed to produce the atonal-associated factor such that a hair cell is generated. An adenoviral vector encoding an atonal-associated factor also is provided.
摘要翻译：本发明涉及一种改变动物的感官知觉的方法。该方法包括施用包含编码无序相关因子的核酸序列的表达载体，所述无序相关因子被表达以产生无调相关因子，从而导致产生允许感知内耳刺激的毛细胞。还提供了一种在体内分化的感觉上皮细胞中产生毛细胞的方法。该方法包括使分化的感觉上皮细胞与腺病毒载体接触（a）E1区和E4区的一个或多个复制必需基因功能缺陷，（b）在E4区包含间隔区，和（ c）包含编码无序相关因子的核酸序列。表达核酸序列以产生无序相关因子，从而产生毛细胞。还提供了编码无机相关因子的腺病毒载体。

4. 发明申请

WO2004050027A2 MATERIALS AND METHODS FOR TREATING OCULAR-RELATED DISORDERS 审中-公开
标题翻译：用于治疗与眼睛有关的疾病的材料和方法
公开(公告)号：WO2004050027A2
公开(公告)日：2004-06-17
申请号：PCT/US2003/038169
申请日：2003-12-01
申请人： GENVEC, INC. , MCVEY, Duncan, L. , BROUGH, Douglas, E. , KOVESDI, Imre , WEI, Lisa
发明人： MCVEY, Duncan, L. , BROUGH, Douglas, E. , KOVESDI, Imre , WEI, Lisa
IPC分类号： A61K
CPC分类号： A61K48/005 , A61K9/0048 , A61K31/203 , A61K31/7088 , A61K38/57 , A61K45/06 , A61K48/00 , A61K48/0058 , A61K48/0075 , A61K48/0083 , C12N15/86 , C12N2710/10343 , C12N2799/022 , A61K2300/00
摘要： The invention is directed to a method of delivering a gene product to an animal. The method comprises administering an expression vector comprising a nucleic acid sequence operably linked to a promoter and encoding a gene product, and upregulating transcription of the nucleic acid sequence in the ocular cell. The expression vector can be an adenoviral vector. The invention further provides a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a neurotrophic agent. In one aspect, the method further comprises upregulating transcription of the nucleic acid sequence. Preferably, if 2x10 8 adenoviral particles of the inventive method are administered to a mouse, the level of expression of the nucleic acid sequence is not diminished more than tenfold at 28 days post-administration.
摘要翻译：本发明涉及将基因产物递送至动物的方法。该方法包括施用包含与启动子可操作地连接并编码基因产物的核酸序列的表达载体，以及上调眼细胞中核酸序列的转录。表达载体可以是腺病毒载体。本发明还提供了预防性或治疗性地治疗动物至少一种眼部相关疾病的方法。该方法包括使眼细胞与包含编码血管发生抑制剂和/或神经营养剂的核酸序列的表达载体接触。在一个方面，该方法还包括上调核酸序列的转录。优选地，如果向小鼠施用本发明方法的2×10 8个腺病毒颗粒，则在给药后28天，核酸序列的表达水平不会降低超过十倍。

5. 发明申请

WO2003020879A3 CELLS AND METHODS FOR PROPAGATING ADENOVIRAL VECTORS 审中-公开
公开(公告)号：WO2003020879A3
公开(公告)日：2003-03-13
申请号：PCT/US2002/024051
申请日：2002-07-22
申请人： GENVEC, INC. , BROUGH, Douglas, E. , GALL, Jason, G., D. , BRUDER, Joseph, T. , KOVESDI, Imre
发明人： BROUGH, Douglas, E. , GALL, Jason, G., D. , BRUDER, Joseph, T. , KOVESDI, Imre
IPC分类号： C12Q1/70
摘要： The invention pertains to cells for the propagation of adenoviral vectors. The invention further provides methods of propagating a replication-deficient adenoviral vector which comprises providing an inventive cell, introducing a replication-deficient adenoviral vector into the inventive cell, and maintaining the cell to propagate the replication-deficient adenoviral vector.

6. 发明申请

WO0158494A3 GENE THERAPY FOR TREATING OCULAR-RELATED DISORDERS 审中-公开
标题翻译：用于治疗与眼睛有关的疾病的基因治疗
公开(公告)号：WO0158494A3
公开(公告)日：2002-04-04
申请号：PCT/US0104203
申请日：2001-02-09
申请人： GENVEC INC , KOVESDI IMRE , BROUGH DOUGLAS E , MCVEY DUNCAN L , WEI LISA
发明人： KOVESDI IMRE , BROUGH DOUGLAS E , MCVEY DUNCAN L , WEI LISA
IPC分类号： C12N15/09 , A61K35/76 , A61K48/00 , A61P27/02 , C07K14/71 , C07K14/81 , C12N15/861 , C07K14/515
CPC分类号： A61K48/0075 , A61K48/005 , C07K14/71 , C07K14/811 , C12N15/86 , C12N2710/10343
摘要： The present invention is directed to a method of prophylactically or therapeutically treating an animal for at least one ocular-related disorder, e.g., ocular neovascularization or age-related macular degeneration. The method comprises contacting an ocular cell with an expression vector comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and the same or different nucleic acid sequence encoding a neurotrophic agent. The method also can comprise contacting an ocular cell with different expression vectors, each comprising a nucleic acid sequence encoding an inhibitor of angiogenesis and/or a nucleic acid sequence encoding a neurotrophic agent. In addition, the present invention provides a viral vector comprising a nucleic acid sequence encoding pigment epithelium-derived factor (PEDF) or a therapeutic fragment thereof.
摘要翻译：本发明涉及一种预防性或治疗性地治疗动物至少一种与眼部相关的疾病，例如眼睛新生血管形成或年龄相关性黄斑变性的方法。该方法包括使眼细胞与包含编码血管生成抑制剂的核酸序列和编码神经营养剂的相同或不同核酸序列的表达载体接触。该方法还可以包括使眼细胞与不同的表达载体接触，每种表达载体包含编码血管生成抑制剂的核酸序列和/或编码神经营养剂的核酸序列。此外，本发明提供了包含编码色素上皮衍生因子（PEDF）的核酸序列或其治疗片段的病毒载体。

7. 发明申请

WO2011057248A3 METHODS OF PROPAGATING MONKEY ADENOVIRAL VECTORS 审中-公开
标题翻译：传播猴子腺病毒载体的方法
公开(公告)号：WO2011057248A3
公开(公告)日：2011-09-15
申请号：PCT/US2010055991
申请日：2010-11-09
申请人： GENVEC INC , GALL JASON , BROUGH DOUGLAS , KAHL CHRISTOPH , MCVEY DUNCAN
发明人： GALL JASON , BROUGH DOUGLAS , KAHL CHRISTOPH , MCVEY DUNCAN
IPC分类号： A61K35/76 , C07K14/075 , C07K14/11 , C07K14/16 , C07K14/35 , C12N5/10 , C12N7/02 , C12N15/861
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2710/10352
摘要： The invention provides methods for propagating a monkey adenovirus in a cell including a human cell, comprising one or more gene products isolated from a human adenovirus. Also provided are methods for propagating wherein the monkey adenovirus comprises a nucleic acid sequence encoding a human adenovirus gene product. The invention further provides a monkey adenovirus. including a replication-deficient monkey adenovirus, obtained by such propagation methods.
摘要翻译：本发明提供了在包括人细胞的细胞中繁殖猴腺病毒的方法，其包含从人腺病毒分离的一种或多种基因产物。还提供了用于繁殖的方法，其中猴腺病毒包含编码人腺病毒基因产物的核酸序列。本发明进一步提供猴腺病毒。包括通过这种繁殖方法获得的复制缺陷型猴腺病毒。

8. 发明申请

WO2011057248A2 SIMIAN ADENOVIRUS AND METHODS OF USE 审中-公开
标题翻译： SIMIAN ADENOVIRUS和使用方法
公开(公告)号：WO2011057248A2
公开(公告)日：2011-05-12
申请号：PCT/US2010/055991
申请日：2010-11-09
申请人： GENVEC, INC. , GALL, Jason , BROUGH, Douglas , KAHL, Christoph , MCVEY, Duncan
发明人： GALL, Jason , BROUGH, Douglas , KAHL, Christoph , MCVEY, Duncan
IPC分类号： A61K35/76
CPC分类号： C07K14/005 , C12N7/00 , C12N15/86 , C12N2710/10322 , C12N2710/10332 , C12N2710/10343 , C12N2710/10352
摘要： The invention provides methods for propagating a monkey adenovirus in a cell including a human cell, comprising one or more gene products isolated from a human adenovirus. Also provided are methods for propagating wherein the monkey adenovirus comprises a nucleic acid sequence encoding a human adenovirus gene product. The invention further provides a monkey adenovirus. including a replication-deficient monkey adenovirus, obtained by such propagation methods.
摘要翻译：本发明提供了在包括人细胞的细胞中繁殖猴腺病毒的方法，其包含从人腺病毒分离的一种或多种基因产物。还提供了用于繁殖的方法，其中猴腺病毒包含编码人腺病毒基因产物的核酸序列。本发明进一步提供猴腺病毒。包括通过这种繁殖方法获得的复制缺陷型猴腺病毒。

9. 发明申请

WO2005012537A2 ADENOVIRAL VECTOR-BASED VACCINES 审中-公开
标题翻译：腺病毒基因疫苗
公开(公告)号：WO2005012537A2
公开(公告)日：2005-02-10
申请号：PCT/US2004/024002
申请日：2004-07-26
申请人： GENVEC, INC. , GALL, Jason, G., D. , WICKHAM, Thomas, J. , ENRIGHT, William, J. , BROUGH, Douglas, E. , ZUBER, Mohammed , KING, C., Richter
发明人： GALL, Jason, G., D. , WICKHAM, Thomas, J. , ENRIGHT, William, J. , BROUGH, Douglas, E. , ZUBER, Mohammed , KING, C., Richter
IPC分类号： C12N15/861
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/5256 , C07K2319/00 , C12N2710/10343 , C12N2710/10345
摘要： The invention provides a method of inducing an immune response in a mammal. The method comprises administering to the mammal a non-subgroup C adenoviral vector comprising an adenoviral fiber protein having an amino acid sequence comprising about 80 % or more identity to an amino acid sequence encoding a subgroup C adenoviral fiber protein. The adenoviral vector further comprises a nucleic acid sequence encoding an antigen which is expressed in the mammal to induce an immune response. The invention further comprises a method of producing an adenoviral vector, and a composition comprising a serotype 41 or a serotype 35 adenoviral vector and a carrier. The invention also provides an adenoviral vector comprising a nucleic acid sequence encoding an adenoviral pIX protein operably linked to a heterologous expression control sequence, as well as a method of enhancing the stability and/or packaging capacity of an adenoviral vector.
摘要翻译：本发明提供在哺乳动物中诱导免疫应答的方法。该方法包括向哺乳动物施用非亚组C腺病毒载体，其包含具有与编码亚组C腺病毒纤维蛋白的氨基酸序列具有约80％或更高同一性的氨基酸序列的腺病毒纤维蛋白。腺病毒载体还包含编码在哺乳动物中表达以诱导免疫应答的抗原的核酸序列。本发明还包括产生腺病毒载体的方法和包含血清型41或血清型35腺病毒载体和载体的组合物。本发明还提供了一种腺病毒载体，其包含编码与异源表达控制序列可操作地连接的腺病毒pIX蛋白的核酸序列，以及增强腺病毒载体的稳定性和/或包装能力的方法。

10. 发明申请

WO1997020051A2 VECTORS AND METHODS FOR GENE TRANSFER TO CELLS 审中-公开
标题翻译：用于基因转移到细胞的载体和方法
公开(公告)号：WO1997020051A2
公开(公告)日：1997-06-05
申请号：PCT/US1996019150
申请日：1996-11-27
申请人： GENVEC, INC. , WICKHAM, Thomas, J. , KOVESDI, Imre , BROUGH, Douglas, E.
发明人： GENVEC, INC.
IPC分类号： C12N15/34
CPC分类号： C12N15/86 , A61K48/00 , C07K14/005 , C07K2319/00 , C12N2710/10322 , C12N2710/10343 , C12N2710/10345 , C12N2810/40 , C12N2810/405 , C12N2810/60
摘要： The present invention provides a chimeric adenovirus coat protein, which differs from the wild-type coat protein by the introduction of a nonnative amino acid sequence. Such a chimeric adenovirus coat protein according to the invention is able to direct entry into cells of a vector comprising the coat protein that is more efficient than entry into cells of a vector that is identical except for comprising a wild-type adenovirus coat protein rather than the chimeric adenovirus coat protein. The chimeric coat protein preferably is a fiber, hexon, or penton protein. The present invention also provides an adenoviral vector that comprises the chimeric adenovirus coat protein, as well as methods of constructing and using such a vector.
摘要翻译：本发明提供了通过引入非氨基酸序列与野生型外壳蛋白不同的嵌合腺病毒外壳蛋白。根据本发明的这种嵌合腺病毒外壳蛋白能够引入包含外壳蛋白的载体的细胞，该载体比除了含有野生型腺病毒外壳蛋白的载体以外的载体的细胞进入更有效，而不是嵌合腺病毒外壳蛋白。嵌合外壳蛋白优选为纤维，六邻体或戊烯隆蛋白。本发明还提供了包含嵌合腺病毒外壳蛋白的腺病毒载体，以及构建和使用这种载体的方法。

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