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1. 发明申请

WO2005038017A2 PREVENTION OF INCORPORATION OF NON-STANDARD AMINO ACIDS INTO PROTEIN 审中-公开
标题翻译：预防将非标准氨基酸并入蛋白质中
公开(公告)号：WO2005038017A2
公开(公告)日：2005-04-28
申请号：PCT/US2004/031224
申请日：2004-09-23
申请人： MONSANTO TECHNOLOGY LLC , BOGOSIAN, Greeg , O'NEIL, Julia, P. , SMITH, Hong, Q.
发明人： BOGOSIAN, Greeg , O'NEIL, Julia, P. , SMITH, Hong, Q.
IPC分类号： C12N9/00
CPC分类号： C12N9/0016 , C07K14/61 , C12P21/02
摘要： The instant invention is drawn to the methods and compositions necessary to provide recombinant proteins with a substantially reduced or eliminated content of norleucine or other non-standard amino acids. Various embodiments of the invention provide for the substantial elimination of the incorporation of non-standard amino acids into recombinant proteins by the co-expression or enhanced expression of a protein (or the enzymatically active portion thereof) capable of degrading norleucine or other non-standard amino acids, including norvaline, beta-methylnorleucine, and homoisoleucine. In certain particular embodiments of the invention, the norleucine is degraded by a glutamate dehydrogenase, a leucine dehydrogenase, a valine dehydrogenase, a phenylalanine dehydrogenase, a glutamate/leucine/phenylalanine/valine dehydrogenase, or an opine dehydrogenase. Also provided are the cells and DNA constructs for carrying out these methods.
摘要翻译：本发明涉及提供具有正亮氨酸或其他非标准氨基酸的含量显着降低或消除的重组蛋白所必需的方法和组合物。本发明的各种实施方案通过共表达或增强的能够降解正亮氨酸或其他非标准蛋白质（或其酶促活性部分）的表达来提供基本消除将非标准氨基酸掺入重组蛋白质中的步骤包括正缬氨酸，β-甲基正亮氨酸和高异亮氨酸。在本发明的某些具体实施方案中，正亮氨酸被谷氨酸脱氢酶，亮氨酸脱氢酶，缬氨酸脱氢酶，苯丙氨酸脱氢酶，谷氨酸/亮氨酸/苯丙氨酸/缬氨酸脱氢酶或冠op碱脱氢酶降解。还提供了用于实施这些方法的细胞和DNA构建体。

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