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    • 10. 发明申请
    • AUTOGRAPHA CALIFORNICA COMPLETE GENOME SEQUENCE
    • AUTOGRAPHA CALIFORNICA完整的基因组序列
    • WO1996001320A2
    • 1996-01-18
    • PCT/IB1995000578
    • 1995-06-30
    • NATURAL ENVIRONMENT RESEARCH COUNCILBISHOP, DavidPOSSEE, RobertAYRES, Martin
    • NATURAL ENVIRONMENT RESEARCH COUNCIL
    • C12N15/34
    • C12N15/86C12N2710/14143
    • The complete nucleotide sequence of the genome of clone 6 of the baculovirus Autographa californica nuclear polyhedrosis virus (AcNPV) has been determined. The molecule comprises 133,894 base-pairs and has an overall A+T content of 59 %. Our analysis suggests that the virus encodes some 154 methionine-initiated, and potentially expressed, open reading frames (ORFs) of 150 nucleotides or greater. These ORFs are distributed evenly throughout the virus genome on either strand. The ORFs are arranged as adjacent, non-overlapping reading frames, separated by short intergenic regions. Based on the primary nucleotide sequence, predictions have been made concerning the functions of certain genes, the sites for initiation of viral DNA replication, the regulation of early and late gene transcription, and factors that may affect the AcNPV gene translational efficiency. The genome sequence data confirm, with minor differences, the information obtained for other AcNPV clones. It is proposed that clone C6 is considered the archetype AcNPV for comparison purposes.
    • 已经确定了杆状病毒苜蓿银纹夜蛾核型多角体病毒(AcNPV)的克隆6的基因组的完整核苷酸序列。 该分子包含133,894个碱基对,并且具有59%的总A + T含量。 我们的分析表明,该病毒编码150个核苷酸或更大的约154个甲硫氨酸引发和潜在表达的开放阅读框(ORF)。 这些ORF在任一条链上均匀分布在病毒基因组上。 ORFs被布置为相邻的非重叠阅读框,由短的基因间区域分隔开。 基于主要核苷酸序列,已经对某些基因的功能,起始病毒DNA复制的位点,早期和晚期基因转录的调节以及可能影响AcNPV基因翻译效率的因素进行了预测。 基因组序列数据证实了其他AcNPV克隆所获得的信息,具有较小的差异。 建议将克隆C6视为原型AcNPV进行比较。