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1. 发明申请

WO2021018111A1 REAGENT CARTRIDGES FOR IN-VITRO DEVICES 审中-公开
公开(公告)号：WO2021018111A1
公开(公告)日：2021-02-04
申请号：PCT/CN2020/105030
申请日：2020-07-28
申请人： BGI SHENZHEN CO., LTD.
发明人： LIN, Sz-Chin , GONG, Jian , OUYANG, Yiwen , LIN, Yan-You
IPC分类号： G01N1/10
摘要： A method and device for displacing fluid from a reagent cartridge (310) into a microfluidic device (320) and for loading the fluid into the reagent cartridge (310). The reagent cartridge (310) may include a cartridge body and a pipette array with pipette tips (315) to engage inlets of a microfluidics or other cartridge, wherein the pipette tips (315) correspond in position to the plurality of inlets (325) of the microfluidic device (320). Fluid may be loaded into or displaced from the microfluidic device (320) by a system of plungers (615). The reagent cartridge may alternatively include blisters (925) having fluid reservoirs and dispensing tips (930), each dispensing tip (930) including a pathway (927) that is fluidly coupled to a blister (925). The fluid may be displaced from or loaded into the blister (925) via the dispensing tip (930). A deformable seal (910) may be overlaid on the blisters (925) to seal the volumes of fluid within the blisters (925), and may be deformed to displace the fluid.

2. 发明申请

WO2021110079A1 IMMERSION SYNTHESIS OF OLIGONUCLEOTIDES 审中-公开
公开(公告)号：WO2021110079A1
公开(公告)日：2021-06-10
申请号：PCT/CN2020/133509
申请日：2020-12-03
申请人： BGI SHENZHEN CO., LTD.
发明人： ALLRED, Benjamin , LI, Handong , ZHANG, Yongwei
IPC分类号： C12Q1/68 , B01J19/00
摘要： Provided are compositions and methods directed to chemically synthesizing oligonucleotides using an array system that contains multiple subset arrays, in which each subset array comprises primers. Each subset array can be selectively immersed in coupling solutions to extend the primers.

3. 发明申请

WO2022073493A1 METHODS AND COMPOSITIONS FOR BIOLUMINESCENCE-BASED SEQUENCING 审中-公开
公开(公告)号：WO2022073493A1
公开(公告)日：2022-04-14
申请号：PCT/CN2021/122738
申请日：2021-10-09
申请人： BGI SHENZHEN CO., LTD.
发明人： CHEN, Yan , LI, Handong , ZHANG, Yongwei
IPC分类号： C12Q1/68
摘要： Disclosed herein are methods and compositions for corresponding positions on an array with differently labeled affinity reagents immobilized at the positions. A first affinity reagent is immobilized at least some of the first positions and a second affinity reagent is immobilized at least some of the second positions. The first affinity reagent is associated with a first luciferase polypeptide and the second affinity reagent is associated with a second luciferase polypeptide. The first luciferase polypeptide does not cross react with the second substrate and the second luciferase polypeptide does not cross react with the first substrate. The method further comprises contacting the array with the first substrate, which reacts with the first luciferase polypeptide and detecting the first luminescent signal at positions, at which the first affinity reagent is immobilized and contacting the array with the second substrate and detecting the second luminescent signal at positions, at which the second affinity reagent is immobilized, thereby corresponding positions on an array with the first affinity reagent or the second affinity reagent immobilized at the positions.

4. 发明申请

WO2021223627A1 SYSTEM AND APPARATUS FOR NANOPORE SINGLE MOLECULE SEQUENCING 审中-公开
公开(公告)号：WO2021223627A1
公开(公告)日：2021-11-11
申请号：PCT/CN2021/090243
申请日：2021-04-27
申请人： BGI SHENZHEN CO., LTD. , BGI SHENZHEN
发明人： LI, Shifeng , KANEKAL, Hemanth , ZHANG, Yuning , YUN, Quanxin
IPC分类号： H03M1/18
摘要： An integrated circuit for controlling a sensor chip capable of sensing various materials includes a plurality of amplifier clusters, a plurality of analog multiplexers, and at least one analog-to-digital converter coupled the analog multiplexers and configured to generate digital code values representative of electrical signals. Each of the amplifier clusters include four amplifiers, each amplifier has a first input coupled to a sensor of the sensor chip, and a second input coupled to a programmable voltage reference. Each one of the analog multiplexers is coupled to one of the amplifier clusters and configured to selectively pass through an electrical signal to the at least one analog-to-digital converter.

5. 发明申请

WO2021143714A1 RAPID MULTIPLEXING CRISPR-BASED DIAGNOSTICS USING MICROFLUIDIC COMPARTMENTALIZATION 审中-公开
公开(公告)号：WO2021143714A1
公开(公告)日：2021-07-22
申请号：PCT/CN2021/071431
申请日：2021-01-13
申请人： MGI TECH CO., LTD. , BGI SHENZHEN CO., LTD.
发明人： JIANG, Yuan , GONG, Jian , PEI, Shao Ning , ZHANG, Yongwei
IPC分类号： C12Q1/6806 , C12N15/09 , C12N15/11 , C12N15/10
摘要： The invention provides a method for detecting the presence of a plurality of different target nucleic acids, comprising the following steps: distributing a portion of a composition comprising nucleic acid into each of a plurality of microfluidic compartments, introducing the Cas endonuclease reaction solution into individual compartments, and detecting the signal changes in the compartments.

6. 发明申请

WO2021037094A1 TEMPERATURE CONTROL ON DIGITAL MICROFLUIDICS DEVICE 审中-公开
公开(公告)号：WO2021037094A1
公开(公告)日：2021-03-04
申请号：PCT/CN2020/111570
申请日：2020-08-27
申请人： BGI SHENZHEN CO., LTD.
发明人： LIN, Yan-You , GONG, Jian , ZHONG, Frank
IPC分类号： C12M1/38 , C12M1/34 , C12M1/00 , C12Q1/68
摘要： A microfluidic device includes first and second substrate structures. The first substrate structure has a first substrate surface configured to receive one or more droplets. A plurality of electrodes configured to apply an electric field to the droplets. The second substrate structure has a second substrate surface facing the first substrate surface and spaced apart from the first substrate surface to form a fluid channel. The microfluidic device has a first heating element adjacent to the first substrate structure and disposed on an opposite side of the first substrate surface, and a second heating element adjacent to the second substrate structure and disposed on an opposite side of the second substrate surface. The microfluidic device further includes one or more temperature sensors disposed adjacent to the fluid channel between the first substrate structure and the second substrate structure.

7. 发明申请

WO2016077313A1 MULTI-PASS SEQUENCING 审中-公开
标题翻译：多级测序
公开(公告)号：WO2016077313A1
公开(公告)日：2016-05-19
申请号：PCT/US2015/059903
申请日：2015-11-10
申请人： BGI SHENZHEN , BGI SHENZHEN CO., LTD.
发明人： TANG, Y. Tom , YU, Jing , JIANG, Hui , ZHANG, Wenwei , FAN, Guangyi , ZHANG, He , MA, Kailong , GENG, Chunyu , LI, Handong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6869 , C12Q2525/151 , C12Q2565/631
摘要： Improved single molecule sequencing methods, compositions, and devices, are provided. In a first aspect, the present invention provides a multi-pass method of sequencing a target sequence using nanopore sequencing, the method comprising: i) providing a non-naturally occurring concatemer nucleic acid molecule comprising a plurality of copies of the target sequence; ii) nanopore sequencing at least three copies of the target sequence in the concatemer, thereby obtaining a multi-pass sequence dataset, wherein the multi-pass sequence dataset comprises target sequence datasets for the at least three copies of the target sequence; and iii) using the multi-pass sequence dataset to determine the target sequence.
摘要翻译：提供改进的单分子测序方法，组合物和装置。在第一方面，本发明提供使用纳米孔测序对靶序列进行测序的多遍方法，所述方法包括：i）提供包含多个靶序列拷贝的非天然存在的连接核酸分子; ii）纳米孔测序所述连接体中靶序列的至少三个拷贝，由此获得多遍序列数据集，其中所述多遍序列数据集包含所述靶序列的所述至少三个拷贝的靶序列数据集; 和iii）使用多遍序列数据集来确定目标序列。

8. 发明申请

WO2021104514A1 ENZYMATIC SYNTHESIS OF OLIGONUCLEOTIDES 审中-公开
公开(公告)号：WO2021104514A1
公开(公告)日：2021-06-03
申请号：PCT/CN2020/132637
申请日：2020-11-30
申请人： BGI SHENZHEN CO., LTD.
发明人： ALLRED, Benjamin , LI, Handong
IPC分类号： C12P19/34 , C40B50/18 , C07H21/00 , C12N9/10
摘要： Provided are compositions and methods directed to using a short template and a polymerase or ligase to synthesize an oligonucleotide. The template is designed such that only one nucleotide is added to the primer in each round of synthesis.

9. 发明申请

WO2021047533A1 OPERATION OF MAGNETIC BEADS ON MICROFLUIDICS SUBSTRATES 审中-公开
公开(公告)号：WO2021047533A1
公开(公告)日：2021-03-18
申请号：PCT/CN2020/114156
申请日：2020-09-09
申请人： BGI SHENZHEN CO., LTD.
发明人： GONG, Jian , LIN, Yan-You , LIN, Sz-Chin , ZHONG, Cheng Frank
IPC分类号： B01L3/00
摘要： Embodiments include methods and apparatuses for separating beads from a droplet main body (46a) on a microfluidics actuator (40) by applying a magnetic field (49) to a droplet (46) disposed at a first location, the droplet (46) including one or more magnetically responsive beads; and moving the magnetic field (49) to separate the one or more magnetically responsive beads from a main body (46a) of the droplet (46). Embodiments also include methods and apparatuses for introducing one or more beads into a droplet main body (46a) by applying a magnetic field (49) to one or more magnetically responsive beads and moving the magnetic field (49) to introduce the one or more magnetically responsive beads into a droplet (46) disposed on a first location, wherein the droplet (46) includes a fluid.

10. 发明申请

WO2021023252A1 ACRIDINIUM ESTER-CONTAINING COMPOUNDS AND METHODS OF USING THE SAME FOR CHEMILUMINESCENCE-BASED ONE-COLOR SEQUENCING 审中-公开
公开(公告)号：WO2021023252A1
公开(公告)日：2021-02-11
申请号：PCT/CN2020/107298
申请日：2020-08-06
申请人： BGI SHENZHEN CO., LTD.
发明人： CHEN, Yan , LI, Handong
IPC分类号： C12Q1/68 , C07H19/00
摘要： Acridinium ester-containing compounds are provided herein. Also provided herein are chemiluminescence-based one-color sequencing methods and methods of using the acridinium ester-containing compounds in chemiluminescence-based one-color sequencing.

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