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1. 发明申请

WO2010118709A2 METHOD OF PRODUCTION OF SYNCHRONIZED ADHERENTLY GROWING CELL LINES AND DEVICE FOR CARRYING OUT SAID METHOD 审中-公开
标题翻译：生产同步生长细胞系的方法和实施方法的装置
公开(公告)号：WO2010118709A2
公开(公告)日：2010-10-21
申请号：PCT/CZ2010/000043
申请日：2010-04-12
申请人： UNIVERZITA PALACKEHO , MISTRIK, Martin , BARTEK, Jiri
发明人： MISTRIK, Martin , BARTEK, Jiri
IPC分类号： C12M3/04
CPC分类号： C12M23/08 , C12M23/46 , C12M27/16 , C12M33/08
摘要： The present invention relates to a method for production of a synchronized adherently growing cell line, wherein a culture bottle with culture medium containing adherent cell line is subjected to a vibration under standard cultivation conditions in an incubator, and after finishing the vibrational treatment, the washed- out cell fraction suspension is removed and transferred to a new culture bottle or taken for analysis. The vibration causes the washout of the poorly adhering mitotic cells to the culture medium and initiates inhibition of their proliferation. The invention further relates to a device for carrying out said method, containing a movable platform (1), flexibly mounted on a stationary support structure (3), said movable platform (1) having attachment means (10) and being connected to a vibrator (5), said vibrator being connected by a cable (6) to a vibration regulator (7) embedded in a box (8), said box (8) holding an energy supply (9).
摘要翻译：本发明涉及一种生产同步贴壁生长细胞系的方法，其中将具有粘附细胞系的培养基的培养瓶在培养箱中在标准培养条件下进行振动，并且在完成振动处理后，洗涤 - 将细胞级分悬浮液除去并转移到新的培养瓶中或用于分析。这种振动使得有丝分裂细胞粘附不均匀的培养液被冲洗并开始抑制其增殖。本发明还涉及一种用于执行所述方法的装置，该装置包含柔性地安装在固定支撑结构（3）上的可移动平台（1），所述可移动平台（1）具有附接装置（10）并连接到振动器（5），所述振动器通过电缆（6）连接到嵌入盒（8）中的振动调节器（7），所述盒（8）保持能量供应（9）。

2. 发明申请

WO2010118709A3 METHOD OF PRODUCTION OF SYNCHRONIZED ADHERENTLY GROWING CELL LINES AND DEVICE FOR CARRYING OUT SAID METHOD 审中-公开
标题翻译：生产同步生长细胞系的方法和实施所述方法的装置
公开(公告)号：WO2010118709A3
公开(公告)日：2010-12-23
申请号：PCT/CZ2010000043
申请日：2010-04-12
申请人： UNIV PALACKEHO , MISTRIK MARTIN , BARTEK JIRI
发明人： MISTRIK MARTIN , BARTEK JIRI
IPC分类号： C12M3/04
CPC分类号： C12M23/08 , C12M23/46 , C12M27/16 , C12M33/08
摘要： The present invention relates to a method for production of a synchronized adherently growing cell line, wherein a culture bottle with culture medium containing adherent cell line is subjected to a vibration under standard cultivation conditions in an incubator, and after finishing the vibrational treatment, the washed- out cell fraction suspension is removed and transferred to a new culture bottle or taken for analysis. The vibration causes the washout of the poorly adhering mitotic cells to the culture medium and initiates inhibition of their proliferation. The invention further relates to a device for carrying out said method, containing a movable platform (1), flexibly mounted on a stationary support structure (3), said movable platform (1) having attachment means (10) and being connected to a vibrator (5), said vibrator being connected by a cable (6) to a vibration regulator (7) embedded in a box (8), said box (8) holding an energy supply (9).
摘要翻译：本发明涉及一种同步贴壁生长细胞系的制造方法，其中将含有贴壁细胞系的培养基的培养瓶在标准培养条件下在恒温箱中进行振动，振动处理结束后， - 除去细胞部分悬浮液并转移到新的培养瓶中或用于分析。振动导致粘附不良的有丝分裂细胞冲洗到培养基中并引发其增殖的抑制。本发明还涉及一种用于执行所述方法的设备，该设备包括柔性地安装在固定支撑结构（3）上的可移动平台（1），所述可移动平台（1）具有连接装置（10）并连接到振动器（5），所述振动器通过电缆（6）连接到嵌入盒子（8）中的振动调节器（7），所述盒子（8）保持能量供应（9）。

3. 发明申请

WO9627008A3 AGENT FOR TREATING TUMOURS AND OTHER HYPERPLASIA AGENT FOR TREATING TUMOURS AND OTHER HYPERPLASIA 审中-公开
标题翻译：用于治疗肿瘤和其他高血压药物的治疗肿瘤和其他高血压药物的代理
公开(公告)号：WO9627008A3
公开(公告)日：1996-11-14
申请号：PCT/DE9600351
申请日：1996-02-26
申请人： MAX PLANCK GESELLSCHAFT , STRAUSS MICHAEL , BARTEK JIRI , LUKAS JIRI , SANDIG VOLKER
发明人： STRAUSS MICHAEL , BARTEK JIRI , LUKAS JIRI , SANDIG VOLKER
IPC分类号： C12N15/09 , A61K31/70 , A61K38/00 , A61K48/00 , A61P35/00 , C07K14/47 , C07K14/82 , C12N15/113 , C12N15/12 , C12Q1/68 , A61K38/17 , C12N15/11
CPC分类号： C12N15/113 , A61K38/00 , A61K48/00 , C07K14/4703 , C07K14/82 , C12N2310/111
摘要： The invention concerns an agent for treating tumours and other hyperplasia. Areas of application of the invention include medicine and the pharmaceutical industry. The agent according to the invention is characterized in that it comprises: a tumour suppressor gene co-operating with the tumour suppressor Rb; an antisense or ribozyme sequence active against antagonistic kinases or cyclines; or another substance which inhibits phosphorylation of the Rb protein.

4. 发明申请

WO1996027008A2 AGENT FOR TREATING TUMOURS AND OTHER HYPERPLASIA 审中-公开
标题翻译：手段治疗肿瘤和其他增生
公开(公告)号：WO1996027008A2
公开(公告)日：1996-09-06
申请号：PCT/DE1996000351
申请日：1996-02-26
申请人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V., BERLIN , STRAUSS, Michael , BARTEK, Jiri , LUKAS, Jiri , SANDIG, Volker
发明人： MAX-PLANCK-GESELLSCHAFT ZUR FÖRDERUNG DER WISSENSCHAFTEN E.V., BERLIN
IPC分类号： C12N15/12
CPC分类号： C12N15/113 , A61K38/00 , A61K48/00 , C07K14/4703 , C07K14/82 , C12N2310/111
摘要： The invention concerns an agent for treating tumours and other hyperplasia. Areas of application of the invention include medicine and the pharmaceutical industry. The agent according to the invention is characterized in that it comprises: a tumour suppressor gene co-operating with the tumour suppressor Rb; an antisense or ribozyme sequence active against antagonistic kinases or cyclines; or another substance which inhibits phosphorylation of the Rb protein.
摘要翻译：本发明涉及用于肿瘤和其它增生的治疗的药剂。本发明的医药和医药行业的应用领域。本发明的试剂的特征在于，它是Rb蛋白的磷酸化中包含与肿瘤抑制RB肿瘤抑制基因，反拮抗激酶和细胞周期蛋白或其他抑制物质的反义或核酶序列的合作。

5. 发明申请

WO0166708A2 MATERIALS AND METHODS RELATING TO THE DEGRADATION OF Cdc25A IN RESPONSE TO DNA DAMAGE 审中-公开
标题翻译：与Cdc25A在DNA损伤反应中的降解相关的材料与方法
公开(公告)号：WO0166708A2
公开(公告)日：2001-09-13
申请号：PCT/GB0101008
申请日：2001-03-08
申请人： ZEALAND PHARMACEUTICALS AS , KIDDLE SIMON , MAILAND NIELS , FALCK JAKOB , BARTEK JIRI , LUKAS JIRI , LUKAS CLAUDIA , SYLJUASEN RANDI
发明人： MAILAND NIELS , FALCK JAKOB , BARTEK JIRI , LUKAS JIRI , LUKAS CLAUDIA , SYLJUASEN RANDI
IPC分类号： A61K38/46 , C12N9/16 , C12N15/55 , G01N33/574 , A61P35/00 , G01N33/68
CPC分类号： C12N9/16 , A61K38/00 , A61K45/06 , C12Y301/03048 , G01N33/574
摘要： Cdc25A has a role in a further signalling pathway for DNA repair which operates in response to DNA damage, in which Chk1 or Chk2 are activated following DNA damage and phosphorylate Cdc25A at one or more serine residues, and more particularly at Ser123 and/or Ser262 and/or Ser292 and/or Ser504. The phosphorylated Cdc25A is then recognised by the F-box protein and is then degraded in a proteasome dependent manner, thereby allowing the cells to undergo cell cycle arrest and repair. Accordingly, by interfering with the phosphorylation and/or degradation of Cdc25A and/or using other strategies to maintain Cdc25A level, this pathway can be used to prevent cells from undergoing repair and thereby increasing the accumulation of DNA damage in the cells, e.g. increasing the fraction of tumour cells which can be killed by DNA damaging therapeutic agents, such as radiation or anti-tumour drugs, or which undergo apoptosis.
摘要翻译： Cdc25A在进一步的DNA修复信号通路中起作用，其作用是响应于DNA损伤，其中Chk1或Chk2在DNA损伤后被激活，并在一个或多个丝氨酸残基上磷酸化Cdc25A，更特别地在Ser123和/或Ser262磷酸化， /或Ser292和/或Ser504。然后磷酸化的Cdc25A被F盒蛋白识别，然后以蛋白酶体依赖的方式降解，从而允许细胞经历细胞周期停滞和修复。因此，通过干扰Cdc25A的磷酸化和/或降解和/或使用其它策略来维持Cdc25A水平，该途径可用于防止细胞经历修复，从而增加细胞中DNA损伤的积累，例如，增加可被DNA破坏性治疗剂如辐射或抗肿瘤药物或可能被凋亡的DNA杀死的肿瘤细胞的分数。

6. 发明申请

WO2003098220A1 MODULATION OF MITOSIS BY ENHANCING THE INTERACTION OF CDC24A AND CYCLINB/CDK1 审中-公开
标题翻译：通过增强CDC24A和CYCLINB / CDK1的相互作用来调控麻醉
公开(公告)号：WO2003098220A1
公开(公告)日：2003-11-27
申请号：PCT/DK2003/000289
申请日：2003-05-02
申请人： ZEALAND PHARMA A/S , LUKAS, Jiri , MAILAND, Niels , BARTEK, Jiri
发明人： MAILAND, Niels , BARTEK, Jiri
IPC分类号： G01N33/574
CPC分类号： A61K38/45 , A61K38/06 , A61K38/55 , A61K45/06 , C12Y207/11022 , G01N33/57484 , G01N2500/02 , G01N2500/04 , A61K2300/00
摘要： The invention generally relates to compositions and methods for modulating entry into mitosis. In one aspect, the invention provides methods for treating or preventing a hyperproliferative disorder in a mammal by promoting the phosphorylation of Cdc25A or the dephosphorylation of cyclin B/Cdk1, or by enhancing the activity of at least one molecular component of the Cdc25A and cyclin B/Cdk1 regulatory interaction/feedback pathway. In another aspect, the invention provides compositions capable of promoting the pathway. The invention has a wide range of important applications including providing screens for compounds that enhance the activity of one or more of the molecular components in the pathway and providing methods for screening a patient population.
摘要翻译：本发明一般涉及用于调节进入有丝分裂的组合物和方法。一方面，本发明提供了通过促进Cdc25A的磷酸化或细胞周期蛋白B / Cdk1的去磷酸化或通过增加Cdc25A和细胞周期蛋白B的至少一种分子成分的活性来治疗或预防哺乳动物中过度增殖性疾病的方法 / Cdk1调节相互作用/反馈途径。另一方面，本发明提供能够促进途径的组合物。本发明具有广泛的重要应用，包括为增强途径中一种或多种分子组分的活性的化合物提供筛选，并提供筛选患者群体的方法。

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