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1. 发明申请

WO0196592A3 REVERSE DETECTION FOR IDENTIFICATION AND/OR QUANTIFICATION OF NUCLEOTIDE TARGET SEQUENCES ON BIOCHIPS 审中-公开
标题翻译：用于鉴定和/或定量生物培养基上核苷酸靶序列的反向检测
公开(公告)号：WO0196592A3
公开(公告)日：2002-04-11
申请号：PCT/BE0100101
申请日：2001-06-14
申请人： UNIV NOTRE DAME DE LA PAIX , REMACLE JOSE , ART MURIEL , LOCKMAN LAURENCE , ZAMMATTEO NATHALIE
发明人： REMACLE JOSE , ART MURIEL , LOCKMAN LAURENCE , ZAMMATTEO NATHALIE
IPC分类号： C12M1/00 , C12Q1/68
CPC分类号： C12Q1/6809 , C12Q1/6827 , C12Q1/6837
摘要： The present invention relates to a method for the identification and/or the quantification of one or more target nucleotide sequences (3) present homologous to each other and being possibly present in a sample and discrimination from possibly homologous sequences, by using two sets of nucleotide sequences (1 and 2), wherein a first set of nucleotide sequences possibly labelled (1) bind specifically to said target nucleotide sequences (3) in a first step and are detected and/or quantified through hybridisation with a second set of capture nucleotide sequences (2) having at least a part of sequence complementary to the possibly labelled nucleotide sequence (1) of the first set of nucleotide sequences, said capture nucleotide sequence (2) being immobilised upon the surface (4) of a solid support according to an array of at least 4 discrete regions/cm , each of said discrete regions being bonded with one species of capture nucleotide sequences (2), and wherein the identification and quantification of the binding between the possibly labelled nucleotide sequence (1) and their corresponding capture nucleotide sequence (2) is correlated with the identification and the quantification of a target nucleotide sequence (3) present in the sample.
摘要翻译：本发明涉及用于鉴定和/或定量一个或多个目标核苷酸序列（3）的方法，所述目标核苷酸序列（3）可以存在于样品中并且可能存在于样品中并且与可能的同源序列区别，通过使用两组核苷酸序列（1和2），其中可能标记（1）的第一组核苷酸序列在第一步中特异性结合所述靶核苷酸序列（3），并通过与第二组捕获核苷酸序列杂交来检测和/或定量（2）具有与第一组核苷酸序列的可能标记的核苷酸序列（1）互补的序列的至少一部分，所述捕获核苷酸序列（2）固定在固体支持物的表面（4）上，根据至少4个离散区域/ cm 2的阵列，每个所述离散区域与一种捕获核苷酸序列（2）结合，并且其中鉴定和量可能标记的核苷酸序列（1）与其相应的捕获核苷酸序列（2）之间的结合的分级与样品中存在的靶核苷酸序列（3）的鉴定和定量相关。

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