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1. 发明申请

WO0242496A2 METHODS AND DEVICES FOR CHARACTERIZING DUPLEX NUCLEIC ACID MOLECULES 审中-公开
标题翻译：用于表征双重核酸分子的方法和装置
公开(公告)号：WO0242496A2
公开(公告)日：2002-05-30
申请号：PCT/US0143309
申请日：2001-11-21
申请人： UNIV CALIFORNIA , HAUSSLER DAVID , WINTERS HILT STEPHEN , AKESON MARK A , VERCOUTERE WENONAH A
发明人： HAUSSLER DAVID , WINTERS-HILT STEPHEN , AKESON MARK A , VERCOUTERE WENONAH A
IPC分类号： C12Q1/68 , G01N27/04
CPC分类号： G01N33/48721 , C12Q1/6825 , C12Q1/6827 , Y10S435/975 , C12Q2565/631 , C12Q2565/607
摘要： Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockage profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acide molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nucleotide polymorphism (SNP), and the sequencing of duplex DNA molecules.
摘要翻译：提供用于表征双链体核酸例如双链体DNA分子的方法和装置。在本主题方法中，包含双链体核酸分子的流体传导介质在施加的电场的影响下与纳米孔接触，并且监测由双链体核酸分子引起的通过纳米孔的电流的变化。观察到的通过纳米孔的电流的变化然后被用作一组数据值以表征双链体核酸，其中该组数据值可以以原始形式使用或被操纵，例如进入当前阻塞曲线。还提供了用于实施本发明方法的纳米孔装置，其中本发明的纳米孔装置的特征在于存在一种算法，其指导处理装置利用监测的通过纳米孔的电流的变化来表征负责当前变化的双相核酸分子。主题方法和装置可用于各种应用，包括除了其他应用之外，样品中分析物双链DNA分子的鉴定，单核苷酸多态性（SNP）的特异碱基序列和双链体DNA的测序分子。

2. 发明申请

WO2013123379A2 NANOPORE SENSOR FOR ENZYME-MEDIATED PROTEIN TRANSLOCATION 审中-公开
标题翻译： NANOPORE传感器用于酶介导的蛋白质转录
公开(公告)号：WO2013123379A2
公开(公告)日：2013-08-22
申请号：PCT/US2013/026414
申请日：2013-02-15
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , NIVALA, Jeffrey, M. , MARKS, Douglas, B. , AKESON, Mark, A.
发明人： NIVALA, Jeffrey, M. , MARKS, Douglas, B. , AKESON, Mark, A.
IPC分类号： C12M1/00
CPC分类号： C12M47/06 , B82Y15/00 , C12Q1/48 , G01N33/48721 , G01N33/54366
摘要： Described herein is a device and method for translocating a protein through a nanopore and monitoring electronic changes caused by different amino acids in the protein. The device comprises a nanopore in a membrane, an amplifier for providing a voltage between the cis side and trans side of the membrane, and an NTP driven unfoldase which processed the protein to be translocated. The exemplified unfoldase is the ClpX unfoldase from E. coli .
摘要翻译：本文描述了通过纳米孔将蛋白质转位并监测由蛋白质中的不同氨基酸引起的电子变化的装置和方法。该装置包括膜中的纳米孔，用于在膜的顺侧和反面之间提供电压的放大器和加工待移位的蛋白质的NTP驱动的非折叠酶。示例性的解折叠酶是来自大肠杆菌的ClpX解折叠酶。

3. 发明申请

WO2006028508A2 METHODS AND APPARATUS FOR CHARACTERIZING POLYNUCLEOTIDES 审中-公开
标题翻译：用于表征多核苷酸的方法和装置
公开(公告)号：WO2006028508A2
公开(公告)日：2006-03-16
申请号：PCT/US2005/009702
申请日：2005-03-23
申请人： PRESIDENT AND FELLOWS OF HARVARD COLLEGE , REGENTS OF THE UNIVERSITY OF CALIFORNIA , AGILENT TECHNOLOGIES, INC. , AKESON, Mark , BRANTON, Daniel , DEAMER, David, W. , SAMPSON, Jeffrey, R.
发明人： AKESON, Mark , BRANTON, Daniel , DEAMER, David, W. , SAMPSON, Jeffrey, R.
IPC分类号： C12Q1/68
CPC分类号： G01N33/48721 , B01L3/502707 , B01L3/502761 , B01L2300/0896 , B01L2400/0475 , B82Y5/00 , B82Y30/00 , C12Q1/6869 , C12Q2565/631 , C12Q2521/543
摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.
摘要翻译：提供了用于分析聚合物的系统和方法，例如多核苷酸。该系统能够以指定的速率分析聚合物。一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

4. 发明申请

WO2012033524A2 CONTROL OF DNA MOVEMENT IN A NANOPORE AT ONE NUCLEOTIDE PRECISION BY A PROCESSIVE ENZYME 审中-公开
标题翻译：控制一个核酶在一个纳米孔中DNA运动的过程酶的精确控制
公开(公告)号：WO2012033524A2
公开(公告)日：2012-03-15
申请号：PCT/US2011/001552
申请日：2011-09-07
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , CHERF, Gerald, Maxwell , LIBERMAN, Kate, R. , LAM, Christopher, Evan , DOODY, Michael , AKESON, Mark, A.
发明人： CHERF, Gerald, Maxwell , LIBERMAN, Kate, R. , LAM, Christopher, Evan , DOODY, Michael , AKESON, Mark, A.
IPC分类号： C12Q1/68 , G01N33/50 , C12N15/09
CPC分类号： C12Q1/6869 , G01N33/48721 , C12Q2537/101 , C12Q2565/631 , C12Q2521/101 , C12Q2563/116
摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the stability of the system in a saline medium and to detect individual nucleotide bases in a polynucleotide in real time and which may be used to sequence DNA for many hours without change of reagents. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的发明提供了可检测和控制混合物中的单独聚合物受纳米孔中的另一化合物（例如酶）作用的装置和方法。特别值得注意的是该系统在盐水培养基中的稳定性并且实时检测多核苷酸中的单个核苷酸碱基，并且可以用来在不改变试剂的情况下将DNA测序数小时。本发明在法医生物学，分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域具有特殊用途。

5. 发明申请

WO2008124107A1 COMPOSITIONS, DEVICES, SYSTEMS, AND METHODS FOR USING A NANOPORE 审中-公开
标题翻译：组合物，设备，系统和使用纳米级的方法
公开(公告)号：WO2008124107A1
公开(公告)日：2008-10-16
申请号：PCT/US2008/004467
申请日：2008-04-04
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , AKESON, Mark, A. , DEAMER, David, W. , DUNBAR, William, B. , BENNER, Seico , CHEN, Roger Jinteh, Arrigo , WILSON, Noah, A. , LIEBERMAN, Kate , ABU-SHUMAYS, Robin , HURT, Nicholas , BRANTON, Daniel
发明人： AKESON, Mark, A. , DEAMER, David, W. , DUNBAR, William, B. , BENNER, Seico , CHEN, Roger Jinteh, Arrigo , WILSON, Noah, A. , LIEBERMAN, Kate , ABU-SHUMAYS, Robin , HURT, Nicholas , BRANTON, Daniel
IPC分类号： G01N33/483 , G01N27/66
CPC分类号： C12Q1/6874 , C12Q1/54 , C12Q1/6869 , C25B3/10 , G01N27/3278 , G01N27/4166 , G01N33/48721 , C12Q2522/101 , C12Q2565/631
摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. The devices and methods are also used to determine rapidly (~ >50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置和方法。所述装置和方法还用于在反馈控制下或使用通过多核苷酸和纳米孔之间的相互作用产生的信号来快速（〜> 50Hz）确定多核苷酸的核苷酸碱基序列。本发明特别用于分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

6. 发明申请

WO2006028508A3 METHODS AND APPARATUS FOR CHARACTERIZING POLYNUCLEOTIDES 审中-公开
标题翻译：用于表征多核苷酸的方法和装置
公开(公告)号：WO2006028508A3
公开(公告)日：2007-02-01
申请号：PCT/US2005009702
申请日：2005-03-23
申请人： HARVARD COLLEGE , UNIV CALIFORNIA , AGILENT TECHNOLOGIES INC , AKESON MARK , BRANTON DANIEL , DEAMER DAVID W , SAMPSON JEFFREY R
发明人： AKESON MARK , BRANTON DANIEL , DEAMER DAVID W , SAMPSON JEFFREY R
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： G01N33/48721 , B01L3/502707 , B01L3/502761 , B01L2300/0896 , B01L2400/0475 , B82Y5/00 , B82Y30/00 , C12Q1/6869 , C12Q2565/631 , C12Q2521/543
摘要： Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture.
摘要翻译：提供了用于分析聚合物的系统和方法，例如多核苷酸。该系统能够以指定的速率分析聚合物。一个这样的分析系统包括具有纳米孔孔的结构和邻近纳米孔孔的分子马达，例如聚合酶。

7. 发明申请

WO2013019759A1 ENZYMATIC PREPARATION OF 10 BASE TO 50 KB DOUBLE-STRAND DNA REAGENT FOR SEQUENCING WITH A NANOPORE-POLYMERASE SEQUENCING DEVICE 审中-公开
标题翻译： 10碱基至50 KB双链DNA试剂的酶促制备，用于使用纳米聚合酶测序装置进行测序
公开(公告)号：WO2013019759A1
公开(公告)日：2013-02-07
申请号：PCT/US2012/048906
申请日：2012-07-30
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , OLSEN, Hugh, E. , AKESON, Mark, A.
发明人： OLSEN, Hugh, E. , AKESON, Mark, A.
IPC分类号： C12Q1/68 , C12N15/11 , G01N33/48
CPC分类号： C12Q1/6869 , C12Q1/6806 , C12Q1/6876 , C12Q2525/119 , C12Q2525/179 , C12Q2525/191 , C12Q2525/301 , C12Q2537/163 , C12Q2565/607 , C12Q2565/631
摘要： The invention herein disclosed provides for devices, reagents, and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the use of reagents to rapidly sequence a polynucleotide. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置，试剂和方法。特别值得注意的是使用试剂快速地对多核苷酸进行测序。本发明特别用于法医生物学，分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

8. 发明申请

WO2012033524A3 CONTROL OF DNA MOVEMENT IN A NANOPORE AT ONE NUCLEOTIDE PRECISION BY A PROCESSIVE ENZYME 审中-公开
标题翻译：通过加工酶在一个核酸精确控制纳豆中的DNA运动
公开(公告)号：WO2012033524A3
公开(公告)日：2012-07-05
申请号：PCT/US2011001552
申请日：2011-09-07
申请人： UNIV CALIFORNIA , CHERF GERALD MAXWELL , LIBERMAN KATE R , LAM CHRISTOPHER EVAN , DOODY MICHAEL , AKESON MARK A
发明人： CHERF GERALD MAXWELL , LIBERMAN KATE R , LAM CHRISTOPHER EVAN , DOODY MICHAEL , AKESON MARK A
IPC分类号： C12Q1/68 , C12N15/09 , G01N33/50
CPC分类号： C12Q1/6869 , G01N33/48721 , C12Q2537/101 , C12Q2565/631 , C12Q2521/101 , C12Q2563/116
摘要： The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the stability of the system in a saline medium and to detect individual nucleotide bases in a polynucleotide in real time and which may be used to sequence DNA for many hours without change of reagents. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof.
摘要翻译：本文公开的本发明提供了可以检测和控制混合物中的单个聚合物在纳米孔中被另一种化合物（例如酶）作用的装置和方法。特别值得注意的是该系统在盐水介质中的稳定性，并实时检测多核苷酸中的单个核苷酸碱基，并可用于在不改变试剂的情况下将DNA序列化数小时。本发明特别用于法医生物学，分子生物学，结构生物学，细胞生物学，分子开关，分子电路和分子计算装置及其制造领域。

9. 发明申请

WO2002042496A3 METHODS AND DEVICES FOR CHARACTERIZING DUPLEX NUCLEIC ACID MOLECULES 审中-公开
公开(公告)号：WO2002042496A3
公开(公告)日：2002-05-30
申请号：PCT/US2001/043309
申请日：2001-11-21
申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA , HAUSSLER, David , WINTERS-HILT, Stephen , AKESON, Mark, A. , VERCOUTERE, Wenonah, A.
发明人： HAUSSLER, David , WINTERS-HILT, Stephen , AKESON, Mark, A. , VERCOUTERE, Wenonah, A.
IPC分类号： C12Q1/68
摘要： Methods and devices are provided for characterizing a duplex nucleic acid, e.g., a duplex DNA molecule. In the subject methods, a fluid conducting medium that includes a duplex nucleic acid molecule is contacted with a nanopore under the influence of an applied electric field and the resulting changes in current through the nanopore caused by the duplex nucleic acid molecule are monitored. The observed changes in current through the nanopore are then employed as a set of data values to characterize the duplex nucleic acid, where the set of data values may be employed in raw form or manipulated, e.g., into a current blockage profile. Also provided are nanopore devices for practicing the subject methods, where the subject nanopore devices are characterized by the presence of an algorithm which directs a processing means to employ monitored changes in current through a nanopore to characterize a duplex nucleic acide molecule responsible for the current changes. The subject methods and devices find use in a variety of applications, including, among other applications, the identification of an analyte duplex DNA molecule in a sample, the specific base sequence at a single nucleotide polymorphism (SNP), and the sequencing of duplex DNA molecules.

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