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2. 发明申请

WO0185992A3 ASSAY FOR CELL CYCLE MODULATORS 审中-公开
标题翻译：细胞周期调节剂的测定
公开(公告)号：WO0185992A3
公开(公告)日：2002-05-23
申请号：PCT/GB0102099
申请日：2001-05-14
申请人： PROLIFIX LTD , VERENIGING HET NL KANKER I , BERNARDS RENE , AGAMI REUVEN
发明人： BERNARDS RENE , AGAMI REUVEN
IPC分类号： G01N33/50 , G01N33/68 , C12Q1/68
CPC分类号： G01N33/5011 , G01N33/6872
摘要： The present invention relates to the finding that cyclin D1 is targeted for destruction in cells which have been exposed to ionising radiation (IR). This finding gives rise to an assay for modulators of cell cycle control, which assay comprises: (a) providing a cell in culture together with apotential modulator compound, said cell expressing a cyclin D1 which undergoes degradation in response to DNAdamage; (b) exposing said cell to a DNA damaging agent; and (c) determining the extent to which the presence of the potential modulator compound inhibits the degradation of said cyclin D1.This finding further gives rise to an assay for modulators of cell cycle control, which assay comprises: (a) providing a cyclin D1, the APC or a componentthereof which interacts with cyclin D1, together with apotential modulator compound; and (b) determining the extent to which the presence of thepotential modulator compound inhibits the interaction ofsaid cyclin D1 and APC or component thereof. In particular where the component of the APC is a protein cdc20.
摘要翻译：本发明涉及细胞周期蛋白D1在已经暴露于电离辐射（IR）的细胞中被破坏的发现。该发现引起细胞周期调控调节剂的测定，该测定法包括：（a）提供培养物中的细胞与潜力调节剂化合物，所述细胞表达细胞周期蛋白D1，其响应于DNA损伤而进行降解; （b）将所述细胞暴露于DNA损伤剂; 并且（c）确定潜在的调节剂化合物的存在程度抑制所述细胞周期蛋白D1的降解。该发现进一步导致细胞周期调控调节剂的测定，该测定包括：（a）提供细胞周期蛋白D1 ，APC或其与细胞周期蛋白D1相互作用的组分，以及潜力调节剂化合物; 和（b）确定潜在调节剂化合物的存在抑制细胞周期蛋白D1和APC或其组分的相互作用的程度。特别是在APC的组分是蛋白质cdc20的情况下。

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