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1. 发明申请

WO2003031626A1 ハロゲン化合物耐性新規ギ酸脱水素酵素及びその製造方法审中-公开
标题翻译：抑制卤素化合物的新型脱氢酶及其生产方法
公开(公告)号：WO2003031626A1
公开(公告)日：2003-04-17
申请号：PCT/JP2002/010460
申请日：2002-10-09
申请人：鐘淵化学工業株式会社 , 難波弘憲 , 高岡康子
发明人：難波弘憲 , 高岡康子
IPC分类号： C12N15/53
CPC分类号： C12N9/0008
摘要： It is intended to provide a formate dehydrogenase which has a high specific activity, a small Km values to formic acid and NAD, a temperature−stability over a wide range, a pH−stability over a wide range, a wide function temperature range and a wide function pH range, is not inactivated even in case of coexisting in an enzymatic reduction system of a haloketone compound, and can efficiently regenerate a coenzyme. A formate dehydrogenase−producing strain is searched for from soil and an enzyme having the above−described properties and suitable for industrial use is provided. Moreover, a DNA containing the gene of this enzyme, a recombinant DNA with a vector and its transformant constructed by using a plasmid are provided. Furthermore, a process for producing the formate dehydrogenase using a strain belonging to the genus Thiobacillus or a transformant constructed by using a formate dehydrogenase gene originating in this strain, and a method of regenerating a coenzyme in an enzymatic reduction system with the use of this enzyme are provided.
摘要翻译：旨在提供具有高比活性，甲酸和NAD的小Km值，宽范围内的温度稳定性，宽范围的pH稳定性，宽功能温度范围和即使在并用于卤代酮化合物的酶还原体系的情况下也不会灭活，能够有效地再生辅酶。从土壤中寻找产生甲酸脱氢酶的菌株，并提供具有上述性质并适于工业用途的酶。此外，提供含有该酶基因的DNA，具有载体的重组DNA及其使用质粒构建的转化体。此外，使用属于硫杆菌属的菌株或通过使用源自该菌株的甲酸脱氢酶基因构建的转化体来生产甲酸脱氢酶的方法，以及在酶促还原中再生辅酶的方法提供了使用该酶的系统。

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