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    • 5. 发明申请
    • METHOD AND ITS COMPOSITIONS FOR DETECTION OF NUCLEIC ACID TARGET FROM BIOLOGICAL SAMPLES AND BODY FLUIDS
    • 用于检测生物样品和体液中核酸靶标的方法及其组合物
    • WO2014060604A2
    • 2014-04-24
    • PCT/EP2013/071906
    • 2013-10-20
    • SELFDIAGNOSTICS OÜ
    • TULP, IndrekKRÕLOV, KatrinLEHES, MarkoLANGEL, Ülo
    • C12Q1/68
    • C12N1/06C12Q1/6806C12Q1/689C12Q2600/16C12Q2531/113
    • Current invention is directed for rapid sample pretreatment method that allows highly sensitive and specific detection of target nucleic acid (e.g. human genomic DNA, human pathogen genomic DNA, human non-pathogen genomic DNA) by amplification directly from crude unpurified biological samples lysates (e.g. human 5 urine, saliva, blood, urethra and cervical swabs and other samples containing biological material). Invention is focused on the description of the biological sample pretreatment method that enables fast release of the genomic material from human and pathogen cells, components of what are compatible with the following nucleic acid amplification method. As an example of the application, 10 invention also discloses protocols and primer sequences for isothermal nucleic acid amplification (recombinase polymerase amplification – RPA, loop-mediated isothermal amplification - LAMP), that enable highly specific and sensitive diagnostics of the genomic material from Homo sapiens, Chlamydia trachomatis and Mycoplasma genitalium from crude biological sample lysates and/or purified 15 total DNA. The example amplification can be combined with immunochromotographic product detection using lateral-flow strips and allows rapid (under 20 min) isothermal nucleic acid amplification based C. trachomatis and M. genitalium diagnostics from human urine samples, that does not require specific laboratory equipment nor qualified personnel, and is therefore well suited 20 for point-of-care settings applications.
    • 本发明涉及快速样品预处理方法,其允许通过直接扩增来对靶核酸(例如人类基因组DNA,人类病原体基因组DNA,人类非病原体基因组DNA)进行高度灵敏和特异性检测 粗制未纯化的生物样品裂解物(例如人类尿液,唾液,血液,尿道和宫颈拭子以及含有生物材料的其他样品)。 本发明集中于生物样品预处理方法的描述,该方法能够快速释放来自人和病原体细胞的基因组材料,这是与下列核酸扩增方法兼容的组分。 作为本申请的一个实例,本发明还公开了用于等温核酸扩增(重组酶聚合酶扩增-RPA,环介导的等温扩增-LAMP)的方案和引物序列,其能够对来自智人的基因组材料进行高度特异性和灵敏的诊断 ,来自粗生物样品裂解物和/或纯化的总DNA的沙眼衣原体和生殖支原体(Mycoplasma genitalium)。 示例性扩增可以与使用横流带的免疫色谱产物检测相结合,并且允许从人尿液样品快速(低于20分钟)的基于等温核酸扩增的沙眼衣原体和生殖器支原体诊断,其不需要特定的实验室设备,也不需要合格 人员,因此非常适合20点的护理设置应用。
    • 6. 发明申请
    • DOSING ACTUATOR FOR AN AEROSOL CONTAINER WITH INTERCHANGEABLE TUBES
    • 具有可交换管的气溶胶容器的调度执行器
    • WO2011138082A1
    • 2011-11-10
    • PCT/EP2011/053744
    • 2011-03-11
    • ONIA ORIGINAL IDEA OÜJUMALOV, Denis
    • JUMALOV, Denis
    • B65D83/30B65D83/16B05B9/08
    • B65D83/202B05B9/0894B65D83/303
    • A universal dosing device with changeable tubes (3) to spray the products kept in bottles (11) comprises housing (2), changeable tubes, an adapter (5), a cover and a control nozzle (7). The dosing device comprises an ergonomic handle with a cavity (9), the top edge thereof has a fixation ring to fix the bottle. The dosing system under the ring comprises a guide canal (15) to install the changeable tube and a trigger (14) that is located underneath them. The rigidity adjustment latch in front of the trigger is attached at least one end of the housing. The support is installed under the canal on the handle. The guide rail between the support and the ring is to make the disassembly easier. The changeable tube is a tube where one end thereof becomes a connection nozzle.
    • 具有用于喷射保持在瓶子(11)中的产品的可变管(3)的通用配量装置包括壳体(2),可变管,适配器(5),盖子和控制喷嘴(7)。 配量装置包括符合人体工程学的手柄,其具有空腔(9),其顶部边缘具有固定环以固定瓶子。 环下的配量系统包括安装可更换管的导管(15)和位于它们下面的触发器(14)。 触发器前面的刚性调节闩锁附接到外壳的至少一端。 支架安装在手柄下的运河下。 支撑和环之间的导轨是使拆卸更容易。 可变管是其一端成为连接喷嘴的管。