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1. 发明申请

WO2012104087A2 CELL CULTURE DEVICE 审中-公开
标题翻译：细胞培养装置
公开(公告)号：WO2012104087A2
公开(公告)日：2012-08-09
申请号：PCT/EP2012/000467
申请日：2012-02-02
申请人： CELLMADE SAS , PAQUAIJ, J., B., G. , BRONSAER, R., J., P.
发明人： PAQUAIJ, J., B., G. , BRONSAER, R., J., P.
IPC分类号： C12M3/00
CPC分类号： C12M35/08 , C12M23/00 , C12M25/02 , C12M25/06
摘要： The invention relates to a cell culture device for culturing together at least a first type of cells and a second, different type of cells, wherein the first type of cells secretes at least a first type of signaling molecules which interacts with the second type of cells and the second type of cells secretes at least a second type of signaling molecules which interacts with the first type of cells, wherein the device comprises a first element having a first surface on which the first type of cells is present and a second element having a second surface on which the second type of cells is present and wherein the first surface and the second surface are separated only by a culture medium and wherein the first element and the second element are detachable.
摘要翻译：本发明涉及用于一起培养至少第一类细胞和第二类不同类型细胞的细胞培养装置，其中第一类细胞分泌至少第一类信号分子其与第二类型的细胞相互作用并且第二类型的细胞分泌与第一类型的细胞相互作用的至少第二类型的信号传导分子，其中所述装置包括具有第一表面的第一元件，第一类型的细胞并且第二元件具有其上存在第二类型的细胞的第二表面，并且其中第一表面和第二表面仅由培养基分开，并且其中第一元件和第二元件是可拆卸的。

2. 发明申请

WO2007045470A1 ENZYMATIC CONVERSION OF OLIGOPEPTIDE AMIDES TO OLIGOPEPTIDE ALKYLESTERS 审中-公开
标题翻译：寡糖类阿片类药物的寡糖转氨酶的酶转化
公开(公告)号：WO2007045470A1
公开(公告)日：2007-04-26
申请号：PCT/EP2006/010089
申请日：2006-10-19
申请人： DSM IP ASSETS B.V. , QUAEDFLIEG, Peter, Jan, Leonard, Mario , SONKE, Theodorus , VERZIJL, Gerardus, Karel, Maria , WIERTZ, Roel, Wim
发明人： QUAEDFLIEG, Peter, Jan, Leonard, Mario , SONKE, Theodorus , VERZIJL, Gerardus, Karel, Maria , WIERTZ, Roel, Wim
IPC分类号： C12P21/00 , C12P7/62
CPC分类号： C12P21/00
摘要： The present invention relates to enzymatic oligopeptide synthesis in the N → C direction, in particular to a process for the preparation of an optionally N-protected oligopeptide C-terminal alkylester comprising the step of reacting the corresponding optionally N-protected oligopeptide C-terminal carboxyamide with an alkyl alcohol, preferably methanol, in the presence of a peptide amidase. The formed C-terminal alkylester can subsequently be used for the coupling with another amino acid residue or oligopeptide. Therefore, inventors have found a very advantageous process for the preparation of oligopeptides.
摘要翻译：本发明涉及在Nα中的酶促寡肽合成特别是制备任选N-保护的寡肽C-端烷基酯的方法，包括使相应的任选N-保护的寡肽C末端羧酰胺与烷基醇，优选甲醇在存在下反应的步骤肽酰胺酶。形成的C-末端烷基酯随后可用于与另一个氨基酸残基或寡肽的偶联。因此，发明人已经发现了制备寡肽的非常有利的方法。

3. 发明申请

WO2006133898A2 IMPROVED BIOSYNTHETIC PRODUCTION OF [5S,6S]-5,6-DIHYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC ACID (2,3-TRANS-CHD) 审中-公开
标题翻译： [5S，6S] -5,6-二羟基环己基-1,3-二苯基-1-羧酸（2,3-TRANS-CHD）的改进的生产生产方法
公开(公告)号：WO2006133898A2
公开(公告)日：2006-12-21
申请号：PCT/EP2006/005664
申请日：2006-06-13
申请人： DSM IP ASSETS B.V. , DSM BIOTECH GMBH , BONGAERTS, Johannes, Josef , RAEVEN, Leon, Jean, Renier, Marie , SPRENGER, Georg , KUHM, Andrea , TAKORS, Ralf , BUJNICKI, Robert, Piotr
发明人： BONGAERTS, Johannes, Josef , RAEVEN, Leon, Jean, Renier, Marie , SPRENGER, Georg , KUHM, Andrea , TAKORS, Ralf , BUJNICKI, Robert, Piotr
IPC分类号： C12P7/42
CPC分类号： C12P7/42
摘要： The invention relates to a process for the biosynthetic production of [5S,6S]-5,6-dihydroxy-cyclohexa-1 ,3-diene-1-carboxylic acid (2,3- trans -CHD) in an Escherichia microorganism having increased activity of isochorismate synthase and isochorismatase, and being deficient in activity of 2,3-dihydroxybenzoate synthase, wherein the process is carried out in a chorismate overproducing host microorganism also having decreased glucose dehydrogenase activity, or is carried out in a chorismate overproducing host microorganism naturally lacking glucose dehydrogenase activity. In this improved biosynthetic process, 2,3- trans -CHD is being produced in high yields and at concentrations in the biosynthetic reaction medium of at least 12 g/l. The invention further relates to the use of the supernatant of a process for the biosynthetic production of [5S,6S]-5,6-dihydroxy-cyclohexa-1 ,3-diene-1-carboxylic acid (2,3- trans -CHD) where the concentration of 2,3-trans-CHD in the process has arrived at a concentration of at least 12 g/l as a source for a co-factor for PQQ dependent enzymes.
摘要翻译：本发明涉及在具有增加的大肠杆菌中的[5S，6S] -5,6-二羟基 - 环己-1,3-二烯-1-羧酸（2,3-反式-CHD）的生物合成生产方法等位基因合成酶和异激酶活性，并且缺乏2,3-二羟基苯甲酸合成酶的活性，其中该方法在也具有降低的葡萄糖脱氢酶活性的过度生产的宿主微生物中进行，或者在过量产生宿主微生物的天竺葵中进行缺乏葡萄糖脱氢酶活性。在这种改进的生物合成过程中，以高产率和生物合成反应介质中的浓度产生至少12g / l的2,3-反式CHD。本发明还涉及生物合成生产[5S，6S] -5,6-二羟基 - 环己-1,3-二烯-1-羧酸（2,3-反式-CHD）的方法的上清液的用途），其中该过程中2,3-反式CHD的浓度已达到至少12g / l的浓度作为PQQ依赖性酶辅因子的来源。

4. 发明申请

WO2012110234A1 FREEZE DRIED EXTRACT OF PARKIA, PREFERABLY OF PARKIA SPECIOSA BEANS FOR TREATMENT OF DISEASES SUCH AS DIABETES MELLITUS TYPE 2 审中-公开
标题翻译： PARKIA冷冻干燥提取物，优选用于治疗疾病的PARKIA SPECIOSA豆类，如糖尿病MELLITUS TYPE 2
公开(公告)号：WO2012110234A1
公开(公告)日：2012-08-23
申请号：PCT/EP2012/000662
申请日：2012-02-15
申请人： BURING DIABETIC CONTROL B.V. , BURING, A. B. , BERG VAN DEN, A. J. J.
发明人： BURING, A. B. , BERG VAN DEN, A. J. J.
IPC分类号： A23L1/30 , A23L3/44
CPC分类号： A61K36/48 , A23B7/024 , A23L3/44 , A23L33/105
摘要： The invention relates to a process for the preparation of a freeze-dried fruit of Parkia, preferably Parkia speciosa comprising the steps of a) freezing a fruit, preferably beans from Parkia to -20°C or below to obtain a frozen fruit and b) subsequently freeze-drying the frozen fruit by reducing the pressure surrounding the said frozen fruit to allow water and volatiles to sublimate and/or evaporate from the frozen fruit to obtain the freeze- dried fruit of Parkia, wherein the temperature of the fruit during freeze-drying is at most 0°C, wherein the temperature of the fruit from the beginning of step a) until the end of step b) does not exceed 30°C, preferably does not exceed 25°C, preferably does not exceed 20°C, more preferably does not exceed 10°C, for example does not exceed 4°C, for example does not exceed 0°C. The invention also relates to the product obtained with said process, a pharmaceutical composition comprising said product, a nutraceutical composition comprising said product, said pharmaceutical composition for use as a medicament, preferably for use in the treatment, co-treatment and/or prevention of diabetes mellitus, to a method of treatment, co-treatment or prevention of diabetes mellitus in animals using said product or said composition and to use of said product or said composition for weight regulation.
摘要翻译：本发明涉及一种用于制备Parkia的冷冻干果的方法，优选是Parkia speciosa，其包括以下步骤：a）将果实（优选来自Parkia的豆）冷冻至-20℃或更低以获得冷冻水果，b）随后通过降低所述冷冻水果周围的压力将冷冻水果冷冻干燥，以使水和挥发物从冷冻水果升华和/或蒸发，得到Parkia的冷冻干燥果实，其中冻结期间水果的温度干燥最多为0℃，其中从步骤a）开始到步骤b）结束的水果的温度不超过30℃，优选不超过25℃，优选不超过20℃ 更优选不超过10℃，例如不超过4℃，例如不超过0℃。本发明还涉及用所述方法获得的产品，包含所述产品的药物组合物，包含所述产品的营养药物组合物，所述药物组合物用作药物，优选用于治疗，共同治疗和/或预防糖尿病，使用所述产品或所述组合物在动物中治疗，共同治疗或预防糖尿病的方法，以及所述产品或所述组合物用于体重调节的方法。

5. 发明申请

WO2006128706A1 BIOSYNTHETIC PRODUCTION OF (5S,6S)-6-AMINO-5-HYDROXYCYCLOHEXA-1,3-DIENE-1-CARBOXYLIC ACID (2,3-CHA) 审中-公开
标题翻译：（5S，6S）-6-氨基-5-羟基环己基-1,3-二苯基-1-羧酸（2,3-CHA）的生产生产
公开(公告)号：WO2006128706A1
公开(公告)日：2006-12-07
申请号：PCT/EP2006/005237
申请日：2006-06-01
申请人： DSM IP ASSETS B.V. , BONGAERTS, Johannes Josef , MÜLLER, Michael , RAEVEN, Leon, Jean, Renier, Marie , LORBACH, Volker
发明人： BONGAERTS, Johannes Josef , MÜLLER, Michael , RAEVEN, Leon, Jean, Renier, Marie , LORBACH, Volker
IPC分类号： C12P13/04 , C07C229/48
CPC分类号： C12P13/04
摘要： The invention relates to a process for the biosynthetic production and recovery of [5S,6S]-6-amino-5-hydroxycyclohexa-1 ,3-diene-1-carboxylic acid) catalyzed, at increased enzyme activity, at least by an enzyme belonging to the class of aminodeoxyisochorismate (ADIC) synthases and an enzyme belonging to the class of isochorismatases, wherein (a) the production is carried out fermentatively with a suitable carbon source as starting material in a chorismate overproducing host microorganism, and with an aminodeoxyisochorismate (ADIC) synthase enzyme selected from the group of such ADIC synthase enzymes that are lacking ADIC lyase activity, and (b) the recovery is carried out by means of concentrating the supernatant of the fermentation to a 2,3-CHA concentration of at least 25 g/l, followed by crystallisation from the said concentrated supernatant. In a second embodiment, the present invention relates to the use of [5S,6S]-6-amino-5-hydroxycyclohexa-1 ,3-diene-1-carboxylic acid (2,3-CHA) as a catalytically active product, in particular as a chiral catalyst. Finally, the invention relates to the novel zinc complex of 2,3-CHA.
摘要翻译：本发明涉及至少通过酶催化生物合成生产和回收[5S，6S] -6-氨基-5-羟基环己-1,3- -1,3-二烯-1-羧酸的方法，其以增加的酶活性催化属于类别的氨基脱氧假单胞菌酸（ADIC）合酶和属于等位基因类别的酶，其中（a）以合适的碳源作为原料在发酵过氧化副产物宿主微生物中以及与氨基脱氧异乳清素 ADIC）合酶，其选自缺少ADIC裂解酶活性的ADIC合成酶组，和（b）通过将发酵的上清液浓缩至至少25的2,3-CHA浓度来进行回收 g / l，随后从所述浓缩的上清液中结晶。在第二个实施方案中，本发明涉及[5S，6S] -6-氨基-5-羟基环己-1,3-二烯-1-羧酸（2,3-CHA）作为催化活性产物的用途，特别是作为手性催化剂。最后，本发明涉及2,3-CHA的新型锌络合物。

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