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1. 发明申请

WO2013103370A1 METHODS AND COMPOSITIONS FOR MODULATING EXPRESSION OR ACTIVITY OF A RKD POLYPEPTIDE IN A PLANT 审中-公开
标题翻译：用于调节植物中RKD多肽的表达或活性的方法和组合物
公开(公告)号：WO2013103370A1
公开(公告)日：2013-07-11
申请号：PCT/US2012/033326
申请日：2012-04-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , CHAMBERLIN, Mark A. , LAWIT, Shai J.
发明人： CHAMBERLIN, Mark A. , LAWIT, Shai J.
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8261 , C12N15/8287
摘要： Methods and compositions are provided to increase the activity/level of an RKD polypeptide or an active variant or fragment thereof in an unreduced ovule plant cell that is outside of the embryo sac. In specific embodiments, such modulation of activity/level of the RKD polypeptide promotes an egg cell-like state in an unreduced ovule plant cell that is outside of the embryo sac. Such methods and compositions can employ an expression construct comprising a RKD polypeptide or active variant or fragment thereof operably linked to an ovule tissue-preferred promoter, in particular an ovule tissue-preferred promoter that is active in at least one non-gametophyte tissue in a plant ovule and is active in an unreduced cell that is outside of the embryo sac.
摘要翻译：提供方法和组合物以增加RKD多肽或其在胚囊外的未还原胚珠植物细胞中的活性变体或片段的活性/水平。在具体实施方案中，RKD多肽的活性/水平的这种调节促进在胚囊外的未还原胚珠植物细胞中的卵细胞样状态。这样的方法和组合物可以使用包含RKD多肽或其活性变体或其片段的表达构建体，其可操作地连接到胚珠组织优选启动子，特别是在至少一种非配子体组织中有活性的胚珠组织优选启动子植物胚珠，并且在胚胎囊外的未减少的细胞中是活跃的。

2. 发明申请

WO2010123667A1 YIELD ENHANCEMENT IN PLANTS BY MODULATION OF A MAIZE TRANSCRIPTION COACTIVATOR P15 (PC4) PROTEIN 审中-公开
标题翻译：玉米转录因子调节剂P15（PC4）蛋白质的植物提取物
公开(公告)号：WO2010123667A1
公开(公告)日：2010-10-28
申请号：PCT/US2010/029560
申请日：2010-04-01
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LI, Guofu
发明人： LI, Guofu
IPC分类号： C12P19/34 , C12N5/00 , C12N15/00
CPC分类号： C07K14/415 , C12N15/8261 , Y02A40/146
摘要： Compositions and methods for modulating plant development and for increasing yield in a plant are provided. The compositions include a PC4 sequence. Compositions of the invention comprise amino acid sequences and nucleotide sequences selected from SEQ ID NOS: 1 and 2 as well as variants and fragments thereof. Nucleotide sequences encoding the PC4 molecule are provided in DNA constructs for expression in a plant of interest are provided for modulating the level of a PC4 sequence in a plant or a plant part are provided. The methods comprise introducing into a plant or plant part a heterologous polynucleotide comprising a PC4 sequence of the invention. The level of the PC4 polypeptide can be increased or decreased. Such method can be used to increase the yield in plants; in one embodiment, the method is used to increase grain yield in cereals.
摘要翻译：提供了用于调节植物发育和提高植物产量的组合物和方法。组合物包括PC4序列。本发明的组合物包含选自SEQ ID NO：1和2的氨基酸序列和核苷酸序列以及其变体和片段。在DNA构建体中提供编码PC4分子的核苷酸序列，以提供用于在植物中表达的植物中的PC4序列的水平或植物部分。所述方法包括向植物或植物部分引入包含本发明的PC4序列的异源多核苷酸。 PC4多肽的水平可以增加或减少。这种方法可以用于提高植物的产量; 在一个实施方案中，该方法用于增加谷物中的谷物产量。

3. 发明申请

WO2010053867A1 NOVEL At1g67330 GENE INVOLVED IN ALTERED NITRATE UPTAKE EFFICIENCY 审中-公开
标题翻译：新的At1g67330基因参与改变硝酸盐摄取效率
公开(公告)号：WO2010053867A1
公开(公告)日：2010-05-14
申请号：PCT/US2009/062978
申请日：2009-11-02
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , FRANK, Mary, J. , SIMMONS, Carl, R.
发明人： FRANK, Mary, J. , SIMMONS, Carl, R.
IPC分类号： A01H5/00 , C12N15/82 , C12N15/63
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/8242 , C12N15/8266 , Y02A40/146
摘要： The invention provides isolated nitrate uptake associated nucleic acids and their encoded proteins for modulating nitrogen uptake efficiency in plants. The invention includes methods and compositions relating to altering nitrogen utilization and/or uptake in plants. The invention further provides recombinant expression cassettes, host cells, and transgenic plants.
摘要翻译：本发明提供了分离的硝酸盐吸收相关核酸及其编码的蛋白质，用于调节植物的氮吸收效率。本发明包括与改变植物中氮利用和/或摄取有关的方法和组合物。本发明还提供了重组表达盒，宿主细胞和转基因植物。

4. 发明申请

WO2008157370A2 SECONDARY WALL FORMING GENES FROM MAIZE AND USES THEREOF 审中-公开
标题翻译：二次形成来自玉米的基因及其用途
公开(公告)号：WO2008157370A2
公开(公告)日：2008-12-24
申请号：PCT/US2008/066946
申请日：2008-06-13
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , DHUGGA, Kanwarpal S. , MEELEY, Robert B. , SIMMONS, Carl R.
发明人： DHUGGA, Kanwarpal S. , MEELEY, Robert B. , SIMMONS, Carl R.
IPC分类号： C12N15/82 , C07K14/415 , A01H5/00 , C12N5/10
CPC分类号： C12N15/8245 , C07K14/415 , C12N15/8261 , Y02A40/146
摘要： The present invention provides polynucleotides and related polypeptides of the class of genes involved in maize secondary wall (ZmSCW) formation. The invention provides genomic sequence for the ZmSCW genes. ZmSCW are responsible for controlling plant growth, secondary cell wall development and yield in crop plants.
摘要翻译：本发明提供涉及玉米次级壁（ZmSCW）形成的基因类的多核苷酸和相关多肽。本发明提供了ZmSCW基因的基因组序列。 ZmSCW负责控制作物植物生长，二次细胞壁发育和产量。

5. 发明申请

WO2014164116A1 FUNCTIONAL EXPRESSION OF BACTERIAL MAJOR FACILITATOR SUPERFAMILY (SFM) GENE IN MAIZE TO IMPROVE AGRONOMIC TRAITS AND GRAIN YIELD 审中-公开
标题翻译：细菌主要辅助物质（SFM）基因的功能表达可改善农业条件和谷物产量
公开(公告)号：WO2014164116A1
公开(公告)日：2014-10-09
申请号：PCT/US2014/020642
申请日：2014-03-05
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY , HOU, Zhenglin , KAHSAY, Robel Y. , LOUSSAERT, Dale , SHEN, Bo , TOMB, Jean-Francois , WANG, Haiyin
发明人： HOU, Zhenglin , KAHSAY, Robel Y. , LOUSSAERT, Dale , SHEN, Bo , TOMB, Jean-Francois , WANG, Haiyin
IPC分类号： C07K14/195 , C12N15/82
CPC分类号： C12N15/8261 , C07K14/195 , C12N15/8273 , Y02A40/146
摘要： Methods for modulating plants using optimized Bacterial MFS constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear tissue growth or kernel number.
摘要翻译：公开了使用优化的细菌MFS构建体调节植物的方法。还公开了核苷酸序列，构建体，载体和修饰的植物细胞，以及显示增加的种子和/或生物量产量的转基因植物，对非生物胁迫如干旱或高植物密度的改善的耐受性，改善的氮利用效率，增加的耳组织生长或核数。

6. 发明申请

WO2014081673A3 ENGINEERING PLANTS FOR EFFICIENT UPTAKE AND UTILIZATION OF UREA TO IMPROVE CROP PRODUCTION 审中-公开
公开(公告)号：WO2014081673A3
公开(公告)日：2014-05-30
申请号：PCT/US2013/070603
申请日：2013-11-18
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , GUPTA, Rajeev , HOU, Zhenglin , LOUSSAERT, Dale F. , SHEN, Bo , WOOD, L. Kent
发明人： GUPTA, Rajeev , HOU, Zhenglin , LOUSSAERT, Dale F. , SHEN, Bo , WOOD, L. Kent
IPC分类号： C12N15/82 , C07K14/195 , C07K14/37 , C07K14/415
摘要： The present disclosure provides polynucleotides and related polypeptides related to urea uptake. The disclosure provides genomic sequences for urea transporter, urease and glutamine synthetase genes. Urea transporters, urease and glutamine synthetase are responsible for controlling nitrogen utilization efficiency in plants. Urea transporter, urease or glutamine synthetase sequences are provided for improving grain yield and plant growth. The disclosure further provides recombinant expression cassettes, host cells and transgenic plants.

7. 发明申请

WO2013138358A1 GENETIC REDUCTION OF MALE FERTILITY IN PLANTS 审中-公开
标题翻译：在植物中遗传减少雄性不育
公开(公告)号：WO2013138358A1
公开(公告)日：2013-09-19
申请号：PCT/US2013/030559
申请日：2013-03-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC.
发明人： FOX, Tim W. , LOUSSAERT, Dale F. , SCHUSSLER, Jeffrey R. , TOLOZA, Juan Pablo San Martin , SHEN, Bo
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8231 , A01H1/02 , C07K14/415 , C12N15/8245 , C12N15/8247 , C12N15/8251 , C12N15/8259 , C12N15/8261 , C12N15/8273 , C12N15/8279 , C12N15/8286 , C12N15/8289
摘要： Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.
摘要翻译：提供了遗传雄性不育植物，其中补体结构导致后代中亲本表型的抑制。提供了产生和维持这种植物的方法和使用所述植物的方法，包括使用亲本植物来生产用于杂交种子生产的无菌植物。

8. 发明申请

WO2013138354A1 GENETIC REDUCTION OF MALE FERTILITY IN PLANTS 审中-公开
标题翻译：遗传减少植物雄性生殖
公开(公告)号：WO2013138354A1
公开(公告)日：2013-09-19
申请号：PCT/US2013/030554
申请日：2013-03-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , E. I. DUPONT DE NEMOURS & COMPANY
发明人： ALBERTSEN, Marc, C. , LEONARD, April, L. , LI, Bailin , SHEN, Bo
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8287 , C07K14/415 , C12N15/8231 , C12N15/8289 , C12Q1/6876 , C12Q2600/13 , C12Q2600/156
摘要： Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.
摘要翻译：提供了遗传雄性不育植物，其中补体构建体导致后代中的亲本表型的抑制。提供了产生和维持这种植物的方法和所述植物的使用方法，包括使用亲本植物生产用于杂交种子生产的不育植物。

9. 发明申请

WO2013103369A1 COMPOSITIONS AND METHODS FOR THE EXPRESSION OF A SEQUENCE IN A REPRODUCTIVE TISSUE OF A PLANT 审中-公开
标题翻译：在植物生殖组织中表达序列的组合物和方法
公开(公告)号：WO2013103369A1
公开(公告)日：2013-07-11
申请号：PCT/US2012/033281
申请日：2012-04-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , ALBERTSEN, Marc C. , CHAMBERLIN, Mark A. , FOX, Timothy W. , LAWIT, Shai J. , LOVELAND, Brian
发明人： ALBERTSEN, Marc C. , CHAMBERLIN, Mark A. , FOX, Timothy W. , LAWIT, Shai J. , LOVELAND, Brian
IPC分类号： C07K14/415 , C12N15/82
CPC分类号： C12N15/8233 , C07K14/415 , C12N15/8234 , C12N15/8263 , C12N15/829
摘要： Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include promoter sequences with direct expression in an egg cell or embryonic cell-preferred manner. Such compositions find use in, for example, a method for expressing a heterologous nucleotide sequence in a plant; detection of specific cell types in the ovule and targeted ablation of specific cell types.
摘要翻译：提供了用于调节植物中异源核苷酸序列表达的组合物和方法。组合物包括在卵细胞中直接表达的启动子序列或胚胎细胞优选的方式。这样的组合物可用于例如在植物中表达异源核苷酸序列的方法; 检测胚珠中的特定细胞类型和特定细胞类型的靶向消融。

10. 发明申请

WO2012142311A1 SELF-REPRODUCING HYBRID PLANTS 审中-公开
标题翻译：自我复制混合植物
公开(公告)号：WO2012142311A1
公开(公告)日：2012-10-18
申请号：PCT/US2012/033357
申请日：2012-04-12
申请人： PIONEER HI-BRED INTERNATIONAL, INC. , LAWIT, Shai J.
发明人： LAWIT, Shai J.
IPC分类号： C12N15/82 , C07K14/415 , A01H1/02 , A01H1/08
CPC分类号： C12N15/8287 , A01H1/02 , A01H1/08 , C07K14/415 , C12N15/8218
摘要： Compositions and methods for the production of self-reproducing hybrid plants are provided. Compositions include suppression cassettes encoding polynucleotides and promoters that result in the MiMe diploid gamete phenotype compositions and suppression cassettes and expression cassettes useful for genome elimination of a parental diploid gamete in a fertilized zygote. The methods involve crossing a first plant comprising a first suppression cassette responsible for producing the MiMe diploid gamete phenotype and a first expression cassette expressing an active CENH3 mutant with a second plant comprising a second suppression cassette that reduces the level of wild-type CENH3 and a second expression cassette comprising a polynucleotide expressing CENH3 specifically in the ovule. Self fertilization of the resultant progeny plant results in the elimination of the female diploid genome in the zygote and normal development of the endosperm. Additionally provided are plants and seeds produced by the methods of the invention.
摘要翻译：提供了生产自我再现混合植物的组合物和方法。组合物包括编码多核苷酸和启动子的抑制盒，其导致MiMe二倍体配子表型组合物和抑制盒和表达盒，其用于在受精受精卵中的亲本二倍体配子的基因组消除。所述方法涉及将包含负责产生MiMe二倍体配子表型的第一抑制盒的第一植物和表达活性CENH3突变体的第一表达盒与含有降低野生型CENH3水平的第二抑制盒的第二植物杂交，第二表达盒包含在胚珠中特异性表达CENH3的多核苷酸。所产生的后代植物的自体受精导致雌性二倍体基因组在受精卵中的消除和胚乳的正常发育。另外提供的是通过本发明的方法生产的植物和种子。

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