专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

81. 发明申请

WO2010012077A1 COMPOSITIONS, METHODS AND KITS FOR REPROGRAMMING SOMATIC CELLS 审中-公开
标题翻译：组合物，方法和用于复制自身细胞的工具
公开(公告)号：WO2010012077A1
公开(公告)日：2010-02-04
申请号：PCT/CA2009/001026
申请日：2009-07-27
申请人： MOUNT SINAI HOSPITAL , UNIVERSITY COURT OF THE UNIVERSITY OF EDINBURGH , NAGY, Andras , KAJI , Keisuke , WOLTJEN, Knut , MICHAEL , Iacovos
发明人： NAGY, Andras , KAJI , Keisuke , WOLTJEN, Knut , MICHAEL , Iacovos
IPC分类号： C12N15/85 , A61K35/12 , C12N15/79 , C12N5/00 , C12N5/10 , C12Q1/02
CPC分类号： G01N33/5014 , C12N5/0696 , C12N15/85 , C12N15/907 , C12N2501/602 , C12N2501/603 , C12N2501/604 , C12N2501/606 , C12N2510/00 , C12N2800/30 , C12N2800/90 , C12N2830/003 , C12N2840/102 , C12N2840/20 , C12N2840/203 , C12N2840/60 , G01N33/5073
摘要： The invention relates to non-viral based vector systems, methods, reprogrammed cells and kits for reprogramming somatic cells. Reprogrammed cells are generated by integrating into somatic cells one or more non-viral based vector systems capable of expressing reprogramming factors necessary to reprogram somatic cells.
摘要翻译：本发明涉及基于非病毒的载体系统，方法，重编程细胞和用于重编程体细胞的试剂盒。通过将能够表达重新编程体细胞所需的重新编程因子的一种或多种基于非病毒的载体系统整合到体细胞中来产生重编程细胞。

82. 发明申请

WO2009150195A9 MULTI-SPECIES POLYNUCLEOTIDE CONTROL SEQUENCES 审中-公开
公开(公告)号：WO2009150195A9
公开(公告)日：2009-12-17
申请号：PCT/EP2009/057225
申请日：2009-06-11
申请人： DSM IP ASSETS B.V. , BERG, VAN DEN, Marco Alexander
发明人： BERG, VAN DEN, Marco Alexander
IPC分类号： C12N15/74 , C12N15/75 , C12N15/79 , C12N15/81 , C12N15/70
摘要： The present invention relates to polynucleotide sequences which enable a polynucleotide control sequence, such as a promoter, to direct expression in a wide range of industrially relevant species, both prokaryotes and eukaryotes. When the polynucleotide sequences of the invention are applied in combination with selection marker genes it is possible to perform selectable cloning in a laboratory host and use the same construct in the final host

83. 发明申请

WO2009135335A1 一种使有丝分裂原活化蛋白激酶失活的方法审中-公开
公开(公告)号：WO2009135335A1
公开(公告)日：2009-11-12
申请号：PCT/CN2008/000904
申请日：2008-05-07
申请人：北京生命科学研究所 , 邵峰 , 李洪涛 , 徐浩 , 周艳
发明人：邵峰 , 李洪涛 , 徐浩 , 周艳
IPC分类号： C12N15/09 , C12N15/79 , A61K48/00 , A61P35/00
CPC分类号： A61K48/005
摘要：本发明公开了一种使有丝分裂原活化蛋白激酶失活的方法。该使有丝分裂原活化蛋白激酶失活的方法，是将含有OspF蛋白和/或SpvC蛋白和/或HopAI1蛋白的编码基因的重组真核表达载体导入目的真核细胞中，使所述目的真核细胞中的有丝分裂原活化蛋白激酶失活。

84. 发明申请

WO2009130598A1 GENETICALLY MODIFIED EUKARYOTIC CELLS 审中-公开
标题翻译：遗传修饰的真核细胞
公开(公告)号：WO2009130598A1
公开(公告)日：2009-10-29
申请号：PCT/IB2009/005495
申请日：2009-04-21
申请人： UniTargeting Research AS , BORTH, Nicole , PRUSS, Thomas , PRYME, Ian, Fraser , STERN, Beate
发明人： BORTH, Nicole , PRUSS, Thomas , PRYME, Ian, Fraser , STERN, Beate
IPC分类号： C12N15/79 , C12N15/67 , C12N5/16
CPC分类号： C12N15/907 , C12N2800/30
摘要： The invention relates to a method of producing genetically modified eukaryotic cells with optimised growth characteristics wherein a recombinant first nucleotide sequence has been integrated into a desired position in the genome. The sequence contains at least one gene encoding a plasma membrane protein with either toxin-receptor or toxic properties and allowing for surface expression based cell sorting to identify a suitable genomic integration locus. The invention also relates to a second exogenous nucleotide sequence containing at least one protein of interest as well as a vector, which aids in the site specific exchange of the first with the second nucleotide sequence. Efficient exchange is achieved by a stringent selection strategy that will kill all cells still expressing the plasma membrane protein.
摘要翻译：本发明涉及一种生产具有优化生长特性的遗传修饰真核细胞的方法，其中重组第一核苷酸序列已经整合到基因组中的所需位置。该序列包含至少一个编码具有毒素受体或毒性的质膜蛋白的基因，并允许基于表面表达的细胞分选来鉴定合适的基因组整合位点。本发明还涉及含有至少一种目的蛋白质的第二种外源核苷酸序列以及有助于第一种与第二种核苷酸序列的位点特异性交换的载体。通过严格的选择策略实现有效的交换，其将杀死仍表达质膜蛋白的所有细胞。

85. 发明申请

WO2009087230A1 DISPLAY VECTORS AND METHODS AND USES THEREOF 审中-公开
标题翻译：显示向量及其方法及其用途
公开(公告)号：WO2009087230A1
公开(公告)日：2009-07-16
申请号：PCT/EP2009/050237
申请日：2009-01-09
申请人： MorphoSys AG , ENZELBERGER, Markus , YODER, Stephen , NEUN, Rüdiger , THIEL, Philippe , PETERS, Solveig
发明人： ENZELBERGER, Markus , YODER, Stephen , NEUN, Rüdiger , THIEL, Philippe , PETERS, Solveig
IPC分类号： C12N15/79
CPC分类号： C12N15/1037
摘要： The present invention relates, in one aspect, to a vector comprising (a) a first polynucleotide capable of encoding a first (poly)peptide comprising at least one cysteine residue, and (b) a second polynucleotide capable of encoding a second (poly)peptide which is a cell surface anchor comprising at least one cysteine residue, wherein the vector is operable in a eukaryotic host cell to express and to cause or allow the attachment of said first (poly)peptide to said second (poly)peptide by formation of a disulfide bond between said cysteine residues comprised within said first (polypeptide and said second (poly)peptide, respectively, wherein said first (poly)peptide is exhibited at the surface of a eukaryotic host cell.
摘要翻译：本发明在一个方面涉及载体，其包含（a）能够编码包含至少一个半胱氨酸残基的第一（多）肽的第一多核苷酸，和（b）能够编码第二（多聚）肽，其是包含至少一个半胱氨酸残基的细胞表面锚，其中所述载体可在真核宿主细胞中操作以表达并引起或允许所述第一（多）肽通过形成所述第二（多）肽而形成分别包含在所述第一（多肽和所述第二（多））肽内的所述半胱氨酸残基之间的二硫键，其中所述第一（多）肽显示在真核宿主细胞的表面。

86. 发明申请

WO2009080720A1 MAMMALIAN EXPRESSION VECTOR 审中-公开
标题翻译：妈妈表达矢量
公开(公告)号：WO2009080720A1
公开(公告)日：2009-07-02
申请号：PCT/EP2008/067947
申请日：2008-12-18
申请人： NOVARTIS AG , KNOPF, Hans-Peter , WILMS, Burkhard
发明人： KNOPF, Hans-Peter , WILMS, Burkhard
IPC分类号： C12N15/79
CPC分类号： C12N15/85
摘要： The invention provides vector nucleic acid for expressing at least one polypeptide of interest in a mammalian cell, comprising (a) at least one expression cassette (POI) for expressing a polypeptide of interest; (b) an expression cassette (MSM) comprising a mammalian selectable marker gene; (c) an expression cassette (MASM) comprising a mammalian amplifiable, selectable marker gene; wherein the expression cassette (POI) is flanked 5' by the expression cassette (MASM), the expression cassette (MSM) is located 3' from the expression cassette (POI) and wherein the expression cassettes (MASM), (POI) and (MSM) are arranged in the same 5' to 3' orientation. Also provided are host cells, comprising said vector and methods for producing a polypeptide using respective host cells.
摘要翻译：本发明提供用于在哺乳动物细胞中表达至少一种目的多肽的载体核酸，其包含（a）用于表达目的多肽的至少一种表达盒（POI）; （b）包含哺乳动物选择标记基因的表达盒（MSM）; （c）包含哺乳动物可扩增的可选择标记基因的表达盒（MASM）; 其中表达盒（POI）由表达盒（MASM）侧接5'，表达盒（MSM）位于表达盒（POI）3'处，其中表达盒（MASM），（POI）和（ MSM）以相同的5'至3'方向排列。还提供了宿主细胞，其包含所述载体和使用各自宿主细胞产生多肽的方法。

87. 发明申请

WO2008115054A2 A METHOD FOR REGULATING EUKARYOTIC GENE EXPRESSION AT THE LEVEL OF CHROMATIN 审中-公开
标题翻译：用于调节染色体上的原核基因表达的方法
公开(公告)号：WO2008115054A2
公开(公告)日：2008-09-25
申请号：PCT/NL2008050150
申请日：2008-03-17
申请人： UNIV AMSTERDAM , VERSCHURE PERNETTE JEANINE , BRINK MAARTJE CAROLIEN
发明人： VERSCHURE PERNETTE JEANINE , BRINK MAARTJE CAROLIEN
IPC分类号： C12Q1/68 , C12N15/79
CPC分类号： C12N15/1086 , C12Q1/6876 , C12Q2565/549 , C12Q2545/114
摘要： The present invention relates to methods for inducing changes in epigenetic gene control in a chromatin domains in eukaryotic cells, and to nucleic acid constructs and host cells for use in such methods. The methods use nucleic acid constructs that are preferablyintegrated at a predetermined chromatin region of the host cell's genome, which nucleic acid construct comprise binding sites for a DNA-binding protein. Proteins that induces or are suspected to induce epigenetic gene control may then be targetedtothe integrated nucleic acid construct by means of fusion to the DNA binding protein. The targeting protein that induces or is suspected to induce epigenetic gene control to the integrated nucleic acid construct then allows to determine its effects on chromatin structure, gene expression, and epigenetic status of the chromatin locus.
摘要翻译：本发明涉及用于诱导真核细胞染色质结构域中的表观遗传基因控制变化的方法，以及用于这些方法的核酸构建体和宿主细胞。所述方法使用优选整合在宿主细胞基因组的预定染色质区域的核酸构建体，该核酸构建体包含DNA结合蛋白的结合位点。诱导或怀疑诱导表观遗传基因控制的蛋白质可以通过与DNA结合蛋白融合而被靶向整合的核酸构建体。诱导或怀疑对整合的核酸构建体诱导表观遗传基因控制的靶向蛋白质允许确定其对染色质基因座的染色质结构，基因表达和表观遗传状态的影响。

88. 发明申请

WO2008079352A2 ANTIBODIES TO CD200R 审中-公开
标题翻译： CD200R抗体
公开(公告)号：WO2008079352A2
公开(公告)日：2008-07-03
申请号：PCT/US2007026202
申请日：2007-12-20
申请人： SCHERING CORP , PRESTA LEONARD G , CHERWINSKI HOLLY M , PHILLIPS JOSEPH H
发明人： PRESTA LEONARD G , CHERWINSKI HOLLY M , PHILLIPS JOSEPH H
IPC分类号： C07K16/28 , A61K39/395 , A61P37/06 , C07K14/705 , C12N5/20 , C12N15/13 , C12N15/79
CPC分类号： C07K16/2803 , A61K2039/505 , C07K2317/24 , C07K2317/56 , C07K2317/565 , C07K2317/76 , C07K2317/92
摘要： The present invention relates to binding compounds specific for the human inhibitory CD200R and uses thereof. More specifically, the invention relates to antibodies that recognize the human inhibitory CD200R and modulates its activity in inflammatory and autoimmune disorders.
摘要翻译：本发明涉及对人抑制性CD200R特异性的结合化合物及其用途。更具体地，本发明涉及识别人抑制性CD200R并调节其在炎性和自身免疫性疾病中的活性的抗体。

89. 发明申请

WO2008048037A1 A NOVEL VECTOR AND EXPRESSION CELL LINE FOR MASS PRODUCTION OF RECOMBINANT PROTEIN AND A PROCESS OF PRODUCING RECOMBINANT PROTEIN USING SAME 审中-公开
标题翻译：用于生产重组蛋白质的新型载体和表达细胞系以及使用其生产重组蛋白的方法
公开(公告)号：WO2008048037A1
公开(公告)日：2008-04-24
申请号：PCT/KR2007/005064
申请日：2007-10-16
申请人： HANMI PHARMACEUTICAL CO., LTD. , CHOI, In Young , KIM, Chang Hwan , LEE, Hyun Ji , PARK, Seong Hee , KWON, Se Chang , LEE, Gwan Sun
发明人： CHOI, In Young , KIM, Chang Hwan , LEE, Hyun Ji , PARK, Seong Hee , KWON, Se Chang , LEE, Gwan Sun
IPC分类号： C12N15/63 , C12N15/79 , C12N15/09
CPC分类号： C12N9/003 , C12N15/85 , C12N2510/00
摘要： Disclosed herein is an inducible high-expression cassette comprising a dihydrofolate reductase (DHFR) promoter from which GC-rich repeat sequences are partially or entirely removed, the cassette capable of more effectively improving a gene amplification system. Also disclosed are an expression vector comprising the inducible expression cassette and optionally a gene encoding a recombinant protein of interest, an animal cell line transformed with the expression vector, and a method of mass producing and purifying a recombinant protein by culturing the transformant. The present invention enables the shortening of the time required to establish a cell line producing a recombinant protein of interest at high levels using a low concentration of a DHFR inhibitor, thereby allowing more effective production of the recombinant protein.
摘要翻译：本文公开了包含二氢叶酸还原酶（DHFR）启动子的诱导型高表达盒，从其中部分或全部除去富含GC的重复序列，所述盒能够更有效地改善基因扩增系统。还公开了包含可诱导表达盒和任选的编码目标重组蛋白的基因，用表达载体转化的动物细胞系的表达载体，以及通过培养转化体大量生产和纯化重组蛋白的方法。本发明能够缩短使用低浓度的DHFR抑制剂以高水平建立产生重组蛋白质的细胞系所需的时间，从而允许更有效地产生重组蛋白质。

90. 发明申请

WO2007069062A3 CASSETTE SYSTEM FOR EXPRESSION CONTROL AND CELL DIFFERENTIATION BY INDUCIBLE RNA INTERFERENCE AND USES THEREOF 审中-公开
标题翻译：用于表达控制的CASSETTE系统和可诱导的RNA干扰的细胞分化及其用途
公开(公告)号：WO2007069062A3
公开(公告)日：2007-11-08
申请号：PCT/IB2006003626
申请日：2006-12-15
申请人： UNIV BASEL , MORONI CHRISTOPH , WEGMUELLER DANIEL
发明人： MORONI CHRISTOPH , WEGMUELLER DANIEL
IPC分类号： C12N15/09 , C12N15/79
CPC分类号： C12N15/85 , C07K14/4702 , C12N15/111 , C12N2310/111 , C12N2320/50 , C12N2800/30 , C12N2800/40 , C12N2830/003
摘要： A regulatory system comprising an integration system with a recombinase target site, such as frt, a marker, such as GFP, and a vector that can integrate into the recombinase target site is disclosed. A regulatory nucleic acid, such as shRNA targeting the production of a certain protein, can be inducibly expressed via the so integrated vector from the site defined by the recombinase target site. The sytem can be used to manipulate regulatory processes in a cell, including cell differentiation.
摘要翻译：公开了包含具有重组酶靶位点（例如frt，诸如GFP的标记）的整合系统和可以整合到重组酶靶位点的载体的调节系统。可以通过由重组酶靶位点定义的位点的如此整合的载体可诱导地表达调节性核酸，例如靶向某种蛋白质的产生的shRNA。该系统可用于操纵细胞中的调节过程，包括细胞分化。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式