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    • 73. 发明申请
    • VITAMIN C PRODUCTION IN MICROORGANISMS AND PLANTS
    • 维生素C生产微生物和植物
    • WO99064618A1
    • 1999-12-16
    • PCT/US1999/011576
    • 1999-05-26
    • A01H5/00C12N1/12C12N1/13C12N1/20C12N5/10C12N9/90C12N15/09C12N15/82C12P17/04C12P19/24C12P19/00C12N5/00C12N5/04
    • C12N9/90C12N15/8243C12P17/04
    • A biosynthetic method for producing vitamin C (ascorbic acid, L-ascorbic acid, or AA) is disclosed. Such a method includes fermentation of a genetically modified microorganism or plant to produce L-ascorbic acid. In particular, the present invention relates to the use of microorganisms and plants having at least one genetic modification to increase the action of an enzyme involved in the ascorbic acid biosynthetic pathway. Included is the use of nucleotide sequences encoding epimerases, including the endogenous GDP-D-mannose:GDP-L-galactose epimerase from the L-ascorbic acid pathway and homologues thereof for the purposes of improving the biosynthetic production of ascorbic acid. The present invention also relates to genetically modified microorganisms, such as strains of microalgae, bacteria and yeast useful for producing L-ascorbic acid, and to genetically modified plants, useful for producing consumable plant food products.
    • 公开了生产维生素C(抗坏血酸,L-抗坏血酸或AA)的生物合成方法。 这种方法包括发酵经遗传修饰的微生物或植物以产生L-抗坏血酸。 特别地,本发明涉及具有至少一种遗传修饰的微生物和植物的用途,以增加参与抗坏血酸生物合成途径的酶的作用。 包括使用编码差向异构酶的核苷酸序列,包括来自L-抗坏血酸途径的内源性GDP-D-甘露糖:GDP-L-半乳糖差向异构酶及其同系物,用于改善抗坏血酸的生物合成生产。 本发明还涉及转基因微生物,例如可用于生产L-抗坏血酸的微藻菌株,细菌和酵母菌,以及用于生产易消化植物食品的转基因植物。